##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631694.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1131297
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60803661637925	34.0	31.0	34.0	31.0	34.0
2	32.76879988190546	34.0	31.0	34.0	31.0	34.0
3	32.85274865928222	34.0	31.0	34.0	31.0	34.0
4	36.28445050238797	37.0	37.0	37.0	35.0	37.0
5	36.20530948106465	37.0	35.0	37.0	35.0	37.0
6	36.25967097941566	37.0	37.0	37.0	35.0	37.0
7	36.25304495636424	37.0	37.0	37.0	35.0	37.0
8	36.23258702179887	37.0	37.0	37.0	35.0	37.0
9	38.06588190369107	39.0	38.0	39.0	37.0	39.0
10	38.000938745528366	39.0	38.0	39.0	35.0	39.0
11	38.0756768558566	39.0	38.0	39.0	37.0	39.0
12	38.00745339199167	39.0	38.0	39.0	35.0	39.0
13	38.06263165198882	39.0	38.0	39.0	35.0	39.0
14	39.430721552342135	40.0	39.0	41.0	37.0	41.0
15	39.44526768832588	40.0	39.0	41.0	37.0	41.0
16	39.388369278801235	40.0	39.0	41.0	37.0	41.0
17	39.37080801946792	40.0	39.0	41.0	37.0	41.0
18	39.407185734603736	40.0	39.0	41.0	37.0	41.0
19	39.42952292810818	41.0	39.0	41.0	37.0	41.0
20	39.43331591969218	41.0	39.0	41.0	37.0	41.0
21	39.393515584324895	40.0	39.0	41.0	37.0	41.0
22	39.364767165474674	40.0	39.0	41.0	36.0	41.0
23	39.298333682490096	40.0	39.0	41.0	36.0	41.0
24	39.31030666571201	40.0	39.0	41.0	36.0	41.0
25	39.2550727174208	40.0	39.0	41.0	36.0	41.0
26	39.185371303910465	40.0	39.0	41.0	36.0	41.0
27	39.063342340693914	40.0	39.0	41.0	35.0	41.0
28	39.05801217540575	40.0	39.0	41.0	35.0	41.0
29	39.017503803156906	40.0	39.0	41.0	35.0	41.0
30	38.96724202397779	40.0	38.0	41.0	35.0	41.0
31	38.866409970149306	40.0	38.0	41.0	35.0	41.0
32	38.80585116021699	40.0	38.0	41.0	35.0	41.0
33	38.732568901004775	40.0	38.0	41.0	35.0	41.0
34	38.69519233234067	40.0	38.0	41.0	35.0	41.0
35	38.608870172907736	40.0	38.0	41.0	35.0	41.0
36	38.55321016496994	40.0	38.0	41.0	35.0	41.0
37	38.494789608741115	40.0	38.0	41.0	35.0	41.0
38	38.449662643850374	40.0	38.0	41.0	35.0	41.0
39	38.35834886859949	40.0	38.0	41.0	34.0	41.0
40	38.02774514561605	40.0	37.0	41.0	34.0	41.0
41	38.06827650033546	40.0	37.0	41.0	34.0	41.0
42	38.028337386203624	40.0	37.0	41.0	34.0	41.0
43	37.55547570620271	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	5.0
18	9.0
19	26.0
20	40.0
21	79.0
22	172.0
23	316.0
24	596.0
25	929.0
26	1620.0
27	2653.0
28	3984.0
29	5887.0
30	8133.0
31	11113.0
32	14629.0
33	19671.0
34	28580.0
35	42795.0
36	65677.0
37	123851.0
38	267614.0
39	532915.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.773913481605625	16.722222369545754	13.363776267416954	27.14008788143167
2	20.118412759867656	20.24154576561239	33.518960980184694	26.121080494335263
3	20.89840245311355	20.792683088525823	29.980190878257435	28.328723580103194
4	14.837041024593894	17.136790780847118	35.0190975490963	33.00707064546269
5	16.28970995238209	33.287633574560886	33.798197997519665	16.62445847553737
6	31.55705354120094	34.793250578760485	16.568770181481963	17.080925698556612
7	28.980806985256745	28.818250203085483	20.848724959051427	21.352217852606344
8	26.729939176007715	31.132496594616622	20.717636482727347	21.419927746648316
9	28.273565650753074	13.292884185143247	18.795948367228057	39.63760179687562
10	19.354510796015546	23.981324090844403	29.774762949075267	26.889402164064784
11	37.45196884637721	20.697040653338604	19.535541948754396	22.315448551529794
12	21.94657989900088	25.01641920733459	27.506127922199035	25.530872971465495
13	34.555647190790744	18.34284012067565	21.843423963822055	25.25808872471155
14	23.40861860324919	21.40755257019156	24.766529037025645	30.417299789533608
15	29.078659273382673	24.777224725248985	21.295026858552617	24.849089142815725
16	23.86676531450185	25.062560936694783	24.094468561306183	26.976205187497182
17	25.476422195055765	24.674687548892997	23.224582050513703	26.624308205537538
18	25.752918994746736	21.994224328359397	24.84714447222966	27.405712204664205
19	27.000071599235216	23.26948626222822	25.276563095279137	24.45387904325743
20	28.84600595599564	21.494267199506407	24.510186096135676	25.149540748362277
21	26.229098105979244	23.131768227088024	23.88302983213073	26.756103834802005
22	26.982039199255368	23.727544579363332	23.411977579716027	25.878438641665273
23	26.663466799611417	22.796489339227453	24.454497802080265	26.085546059080862
24	26.32712718234027	22.535196327754782	25.06371006022291	26.073966429682038
25	26.266046847114417	23.07652190362036	25.106050842528532	25.55138040673669
26	26.233164235386464	23.85960539098044	24.408621255072717	25.498609118560378
27	25.65206130662417	22.83635508624172	26.04479637089111	25.466787236243004
28	25.04240707789378	24.00386459081921	23.986804526132392	26.966923805154615
29	25.67734202424297	24.514694196130634	24.234131267032442	25.573832512593953
30	25.6400397066376	22.98158662137352	25.661961447789572	25.716412224199303
31	26.489860752746626	23.295739315140057	24.573122707830038	25.641277224283275
32	24.166332978872923	23.277441732807567	25.107465148409304	27.44876013991021
33	24.42532774328934	22.839714062708556	26.683620658412423	26.05133753558968
34	24.86659117809028	22.927666209669077	25.93076795925385	26.274974652986792
35	24.319696772819164	24.264715631704142	26.35523651172062	25.06035108375608
36	24.775722025250662	22.888507615595195	26.76070032891451	25.575070030239626
37	25.102338289591504	22.621468986481887	26.31422164117822	25.961971082748388
38	24.424708984466502	21.935530634307348	27.121525116746533	26.518235264479618
39	23.96718103203668	21.029579323555176	28.425249956465898	26.57798968794225
40	23.718793561726052	22.11417514587239	28.643495032692563	25.52353625970899
41	21.922448304910205	21.576650517061392	29.857146266630245	26.643754911398155
42	20.58433815346456	22.321017380935334	30.185088442734315	26.90955602286579
43	19.936586060070873	23.168274997635457	29.695738607987117	27.19940033430655
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	36.0
1	38.0
2	40.0
3	109.0
4	178.0
5	178.0
6	268.5
7	359.0
8	392.5
9	426.0
10	682.5
11	939.0
12	939.0
13	1886.0
14	2833.0
15	5436.0
16	8039.0
17	7820.5
18	7602.0
19	7602.0
20	8316.5
21	9031.0
22	7252.5
23	5474.0
24	5483.0
25	5492.0
26	5492.0
27	5988.0
28	6484.0
29	7631.5
30	8779.0
31	10306.0
32	11833.0
33	11833.0
34	14672.0
35	17511.0
36	19178.5
37	20846.0
38	25465.5
39	30085.0
40	30085.0
41	36152.0
42	42219.0
43	49007.5
44	55796.0
45	75227.0
46	94658.0
47	94658.0
48	104486.5
49	114315.0
50	126771.0
51	139227.0
52	136021.0
53	132815.0
54	132815.0
55	116695.0
56	100575.0
57	92009.0
58	83443.0
59	75950.0
60	68457.0
61	68457.0
62	62038.5
63	55620.0
64	44934.5
65	34249.0
66	29614.5
67	24980.0
68	24980.0
69	21100.5
70	17221.0
71	14668.0
72	12115.0
73	9767.0
74	7419.0
75	7419.0
76	6186.0
77	4953.0
78	3999.5
79	3046.0
80	2403.5
81	1761.0
82	1761.0
83	1443.0
84	1125.0
85	894.5
86	664.0
87	513.0
88	362.0
89	362.0
90	293.0
91	224.0
92	139.0
93	54.0
94	32.5
95	11.0
96	11.0
97	6.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1131297.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.603520666132916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.08193085373492	35.397078685878526
2	6.977419581861409	5.805704395405033
3	2.3597080228762337	2.9451648448731307
4	1.2028042292425534	2.0016356243441855
5	0.7413953912435176	1.5422329240687693
6	0.5010923896417244	1.2508324552860848
7	0.36502279200790394	1.063036328963726
8	0.290475123473675	0.9667830241947638
9	0.2375630652730663	0.8895113906038107
>10	1.797269248466335	15.106279553328827
>50	0.2420093830458118	7.117614397097258
>100	0.17551781664243846	14.207457878018362
>500	0.01838554472445045	5.147799814039016
>1k	0.009406557765997905	6.55886868389853
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4412	0.3899948466229469	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4028	0.35605150548441306	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3550	0.31379911729634213	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2864	0.2531607526582321	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2760	0.2439677644332125	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2624	0.23194616444664842	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2507	0.22160405269350136	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	2288	0.20224574095043124	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	2214	0.19570457625185958	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2178	0.19252238802012203	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	2170	0.1918152350797359	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	2094	0.18509728214606774	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1978	0.17484356451046895	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1791	0.15831386452894333	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1747	0.15442452335681967	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1701	0.15035839394959943	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1608	0.14213774101761076	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1486	0.13135365867672238	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1390	0.12286782339208889	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1290	0.11402841163726236	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	1284	0.11349804693197277	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	1273	0.11252571163894184	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1273	0.11252571163894184	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1252	0.11066943517042828	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	1245	0.11005067634759043	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	1240	0.1096087057598491	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1202	0.10624972929301502	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1176	0.10395148223676012	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	1154	0.10200681165069828	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	1148	0.10147644694540868	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	1146	0.10129965871031214	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	8.83941175482654E-5	0.0	0.0	0.0
6	0.0	8.83941175482654E-5	0.0	0.0	0.0
7	0.0	8.83941175482654E-5	0.0	0.0	0.0
8	4.41970587741327E-4	8.83941175482654E-5	0.0	0.0	0.0
9	4.41970587741327E-4	8.83941175482654E-5	0.0	0.0	0.0
10	4.41970587741327E-4	8.83941175482654E-5	0.0	0.0	0.0
11	4.41970587741327E-4	8.83941175482654E-5	0.0	8.83941175482654E-5	0.0
12	4.41970587741327E-4	8.83941175482654E-5	0.0	1.767882350965308E-4	0.0
13	4.41970587741327E-4	8.83941175482654E-5	0.0	2.651823526447962E-4	0.0
14	4.41970587741327E-4	8.83941175482654E-5	0.0	4.41970587741327E-4	0.0
15	6.187588228378578E-4	8.83941175482654E-5	0.0	6.187588228378578E-4	0.0
16	6.187588228378578E-4	8.83941175482654E-5	0.0	0.001325911763223981	0.0
17	6.187588228378578E-4	8.83941175482654E-5	0.0	0.0024750352913514313	0.0
18	7.071529403861232E-4	8.83941175482654E-5	0.0	0.0030053999966410234	0.0
19	7.071529403861232E-4	8.83941175482654E-5	0.0	0.003977735289671943	0.0
20	7.071529403861232E-4	8.83941175482654E-5	0.0	0.005657223523088986	0.0
21	7.071529403861232E-4	8.83941175482654E-5	0.0	0.009546564695212662	0.0
22	7.071529403861232E-4	8.83941175482654E-5	0.0	0.013789482337529402	0.0
23	7.071529403861232E-4	8.83941175482654E-5	0.0	0.01670648821662216	0.0
24	7.071529403861232E-4	8.83941175482654E-5	0.0	0.021214588211583695	0.0
25	7.071529403861232E-4	8.83941175482654E-5	0.0	0.023159258797645535	0.0
26	7.071529403861232E-4	8.83941175482654E-5	0.0	0.028728088203186255	0.0
27	7.071529403861232E-4	8.83941175482654E-5	0.0	0.058340117581855165	0.0
28	7.071529403861232E-4	8.83941175482654E-5	0.0	0.1877491056725157	0.0
29	7.071529403861232E-4	8.83941175482654E-5	0.0	0.4064361524869243	0.0
30	7.071529403861232E-4	8.83941175482654E-5	0.0	0.6733863874826858	0.0
31	7.955470579343886E-4	8.83941175482654E-5	0.0	1.4531108983759349	8.83941175482654E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2380	0.0	29.382353	1
GTATAGA	35	8.86931E-4	26.42857	1
CTAGTTC	120	1.0913936E-11	21.583332	3
CTTATAC	1765	0.0	21.172804	37
CTATACA	45	0.0038257707	20.555557	4
GCGGTAT	65	6.902009E-5	19.923077	6
GTATCAA	3570	0.0	19.536413	2
GCGGTAA	1045	0.0	19.473684	23
TAGTTCC	125	4.129106E-10	19.24	4
ACACGAA	145	7.2759576E-12	19.13793	17
GCAGTCG	510	0.0	18.862745	9
ACAACTA	50	0.007034815	18.5	8
GTTCTAG	130	6.9667294E-10	18.5	1
GTTAGCA	50	0.007034815	18.5	2
TGTTACG	110	3.851528E-8	18.5	16
ACAGATC	50	0.007034815	18.5	8
CAGTCGG	520	0.0	18.5	10
AAACACG	150	1.2732926E-11	18.499998	15
CGCGGTA	1110	0.0	18.333334	22
AGTCGGT	520	0.0	18.14423	11
>>END_MODULE