FastQCFastQC Report
Fri 10 Feb 2017
ERR1631693.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631693.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395724
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8680.21934479586782707No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8170.20645702560370358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.20544622009279195No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6210.15692755556903296No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4810.12154936268712536No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4720.11927505028757417No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4540.11472642548847176No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4250.10739808553436234No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4190.10588187726799486No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3970.10032244695798083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4400.031.534091
GACCTTA358.859076E-426.4285727
TAGATTC451.3210715E-424.6666665
GTCCGGC400.001928961723.12500223
TTGCATC400.001928961723.12500233
TATACAC1300.022.7692337
CTTAGGA753.727946E-722.22
GGTCCGG502.6977662E-422.19999922
GCGGTCC450.00382144320.55555520
TAGACAG450.00382144320.5555555
TTGGGAC450.00382144320.5555553
CTAGATT555.134908E-420.1818184
GGACCGT555.134908E-420.1818186
TGTACTG555.134908E-420.1818185
GTGCTAG759.2406135E-619.7333341
TTAGGAC851.2410892E-619.5882363
CTTATAC5350.019.01869237
GTATCAA7300.018.7534262
GTCGCCA701.2166349E-418.512
GCGGTCT500.00702693318.49999818