FastQCFastQC Report
Fri 10 Feb 2017
ERR1631692.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631692.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336819
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9110.27047167766663993No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8310.24672004845332363No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8040.2387038735938293No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7340.2179211980321775No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6840.2030764297738548No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6730.19981058075702382No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6670.19802920856602507No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6110.18140306811670362No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5780.17160552106621063No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5620.1668551952235474No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5620.1668551952235474No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5240.1555731713472221No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5140.15260421769555757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.14488493820122975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4640.13775944943723484No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3860.11460161095425139No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3820.11341402949358557No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3690.10955438974642168No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3630.10777301755542294No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3580.10628854072959068No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3550.10539785463409132No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3510.10421027317342549No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3480.10331958707792611No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3460.10272579634759323No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3370.10005373806109513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATG200.001840089337.015
TCCTACT250.005490895429.62
GCTTTAG250.005490895429.61
GGTATCA5150.026.941751
GTACTGG1950.020.8717961
CGCGAGC450.003820279120.55555512
TTCATTG450.003820279120.5555553
ATAGAGC555.132724E-420.18181830
GCGGTAA3400.020.13235323
ACTGTGC656.883631E-519.9230778
CGCGGTA3450.019.8405822
GTATCAA7050.019.6808512
CTGTACT500.0070248118.54
TCCTGTA500.0070248118.52
TAGAACA500.0070248118.55
GCCTAGT500.0070248118.51
CGGTAAT3700.018.524
GCGCTAT500.0070248118.56
AGCCGCG3750.018.25333219
GGTAATA3800.018.01315925
Produced by FastQC (version 0.11.5)