FastQCFastQC Report
Fri 10 Feb 2017
ERR1631691.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631691.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529975
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27680.18091798885602706No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA24460.15987189333159038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24250.1584993218843445No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA23890.15614634226049445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22240.14536185231784832No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA20340.13294334874752856No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA19610.1281720289547215No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19110.12490400169937418No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA17510.11444631448226279No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA17370.11353126685076553No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC17340.1133351852154447No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16990.11104756613670157No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA16740.10941355250902791No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC15390.10058987891959019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.030.105591
GTACTAG250.005496905729.5999986
TTACTCG855.183574E-821.76470629
TATCCGG1001.290573E-820.34999810
AACCGTA555.144164E-420.1818187
CTTATAC15850.020.07570837
GTACTAT656.904038E-519.9230771
ATACGGC1303.274181E-1119.92307729
GCGGTAA10400.019.74519223
AAACACG851.2458604E-619.58823615
CTATCCG1052.260822E-819.380959
CCGACGT1052.260822E-819.3809523
GCAGTCG4150.019.1686749
TTCTGCG8100.018.9567918
CAGTCGG4200.018.94047510
CGCGGTA11200.018.522
TTAGACT609.2387205E-418.54
AAGACCG701.2195326E-418.55
TTATATT902.153165E-618.54
AGTCGGT4300.018.511
Produced by FastQC (version 0.11.5)