##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631691.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1529975
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63747185411526	34.0	31.0	34.0	31.0	34.0
2	32.80367391624046	34.0	31.0	34.0	31.0	34.0
3	32.89778983316721	34.0	31.0	34.0	31.0	34.0
4	36.30576839490841	37.0	37.0	37.0	35.0	37.0
5	36.22786450758999	37.0	35.0	37.0	35.0	37.0
6	36.27818951290054	37.0	37.0	37.0	35.0	37.0
7	36.26361084331443	37.0	37.0	37.0	35.0	37.0
8	36.24506544224579	37.0	37.0	37.0	35.0	37.0
9	38.07433324073923	39.0	38.0	39.0	37.0	39.0
10	38.0144237650942	39.0	38.0	39.0	35.0	39.0
11	38.08439222863119	39.0	38.0	39.0	37.0	39.0
12	38.026230493962316	39.0	38.0	39.0	35.0	39.0
13	38.07170640043138	39.0	38.0	39.0	37.0	39.0
14	39.485839964705306	41.0	39.0	41.0	37.0	41.0
15	39.488767463520645	41.0	39.0	41.0	37.0	41.0
16	39.441617019885946	41.0	39.0	41.0	37.0	41.0
17	39.41030147551431	41.0	39.0	41.0	37.0	41.0
18	39.42833902514747	41.0	39.0	41.0	37.0	41.0
19	39.459159136587196	41.0	39.0	41.0	37.0	41.0
20	39.448134119838556	41.0	39.0	41.0	37.0	41.0
21	39.413302178140164	41.0	39.0	41.0	37.0	41.0
22	39.400606545858594	40.0	39.0	41.0	37.0	41.0
23	39.329458324482424	40.0	39.0	41.0	36.0	41.0
24	39.34595990130558	41.0	39.0	41.0	36.0	41.0
25	39.29306688017778	40.0	39.0	41.0	36.0	41.0
26	39.23200379091162	40.0	39.0	41.0	36.0	41.0
27	39.125545188646875	40.0	39.0	41.0	36.0	41.0
28	39.11683197437867	40.0	39.0	41.0	36.0	41.0
29	39.093257079364044	40.0	39.0	41.0	35.0	41.0
30	39.05224856615304	40.0	39.0	41.0	35.0	41.0
31	38.96912041046422	40.0	39.0	41.0	35.0	41.0
32	38.91784702364418	40.0	38.0	41.0	35.0	41.0
33	38.8558316312358	40.0	38.0	41.0	35.0	41.0
34	38.82488994918218	40.0	38.0	41.0	35.0	41.0
35	38.75817774800242	40.0	38.0	41.0	35.0	41.0
36	38.711198548995895	40.0	38.0	41.0	35.0	41.0
37	38.67464697135574	40.0	38.0	41.0	35.0	41.0
38	38.629706367751105	40.0	38.0	41.0	35.0	41.0
39	38.56298566970049	40.0	38.0	41.0	35.0	41.0
40	38.25390610957695	40.0	38.0	41.0	34.0	41.0
41	38.32614127681825	40.0	38.0	41.0	34.0	41.0
42	38.311943659210115	40.0	38.0	41.0	34.0	41.0
43	37.848592297259756	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	1.0
13	1.0
14	0.0
15	1.0
16	1.0
17	8.0
18	6.0
19	25.0
20	53.0
21	95.0
22	239.0
23	399.0
24	723.0
25	1227.0
26	2092.0
27	3067.0
28	4751.0
29	7097.0
30	10123.0
31	13748.0
32	18614.0
33	25049.0
34	36882.0
35	55386.0
36	86499.0
37	156678.0
38	352798.0
39	754408.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.70135132927009	16.834980963741238	13.010408666808281	27.453259040180395
2	19.57306491936143	19.87842938610108	34.355855487834766	26.192650206702723
3	20.504975571496267	20.61746106962532	29.764603996797334	29.11295936208108
4	15.173973430938414	16.012810666840963	34.05571986470367	34.75749603751696
5	16.245102044151047	34.043236000588244	33.48956682298731	16.222095132273402
6	33.95499926469387	33.99924835373127	15.820258500955898	16.225493880618963
7	30.087027565809898	28.723606594879	20.38719586921355	20.80216997009755
8	26.78259448683802	32.41562770633507	20.222748737724473	20.579029069102436
9	27.798101276164644	13.997875782284025	18.131211294302194	40.07281164724914
10	18.633114920178436	24.821451330904097	30.534093694341415	26.011340054576053
11	37.75081292177977	20.435431951502476	20.046732789751466	21.767022336966292
12	22.415856468242946	24.41451657706825	27.43025212830275	25.73937482638605
13	33.24557590810308	18.344221310805732	22.488275952221443	25.92192682886975
14	23.5248288370725	20.127387702413436	23.959476462033695	32.38830699848037
15	28.144969689047205	25.31904116080328	21.133613294334875	25.402375855814636
16	25.243549731204755	24.81125508586742	23.00266344221311	26.94253174071472
17	25.977287210575334	24.644193532574064	22.861288583146784	26.517230673703818
18	25.5817251915881	22.869197209104726	24.56955179006193	26.979525809245246
19	27.373453814604815	23.31704766417752	24.52327652412621	24.786221997091456
20	28.20745437016945	22.184087975293714	24.295495024428504	25.312962630108338
21	26.199970587754702	23.64169349172372	23.256393078318276	26.9019428422033
22	26.812856419222538	23.61005898789196	22.79566659585941	26.781417997026097
23	26.2884034052844	23.566986388666482	23.76215297635582	26.382457229693294
24	27.084364123596792	22.968545237667282	23.831173711988757	26.11591692674717
25	27.003970653115246	23.027565809898853	24.184120655566268	25.78434288141963
26	26.830046242585663	23.51757381656563	23.664373600875834	25.988006339972873
27	26.10689717152241	22.974754489452444	24.683475220183336	26.23487311884181
28	25.502900374189117	23.81529109952777	23.734047941959837	26.947760584323273
29	26.103694504812168	24.50589061912776	23.57581006225592	25.81460481380415
30	25.71545286687691	23.84012810666841	24.378895079984968	26.065523946469714
31	26.714161996111045	23.38469582836321	24.052942041536625	25.848200133989117
32	24.541708197846372	23.422539583980132	24.472033856762366	27.563718361411134
33	25.07295870847563	22.856255821173548	25.444598767953725	26.6261867023971
34	25.477017598326768	22.975734897629046	25.020474190754747	26.526773313289432
35	25.2533538129708	23.84718704553996	25.090671416199612	25.808787725289626
36	25.28505367734767	23.08155362015719	25.690681220281377	25.94271148221376
37	25.943365087664834	22.237552901191197	25.248386411542672	26.5706955996013
38	24.910864556610402	22.100295756466608	26.469778917956177	26.519060768966813
39	24.48412555760715	21.253484534061016	27.38025131129594	26.882138597035897
40	24.243010506707627	21.841794800568636	27.995490122387622	25.919704570336116
41	22.941747414173435	21.648523668687396	28.824980800339873	26.584748116799297
42	22.212389091325022	21.729440023529797	29.054527034755466	27.003643850389714
43	21.28675305152045	22.37056161048383	29.131456396346344	27.211228941649374
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	23.0
2	19.0
3	70.5
4	122.0
5	122.0
6	176.0
7	230.0
8	241.0
9	252.0
10	435.5
11	619.0
12	619.0
13	1171.0
14	1723.0
15	3523.0
16	5323.0
17	5246.0
18	5169.0
19	5169.0
20	5681.5
21	6194.0
22	5789.0
23	5384.0
24	5953.0
25	6522.0
26	6522.0
27	7593.0
28	8664.0
29	12479.0
30	16294.0
31	18331.0
32	20368.0
33	20368.0
34	24789.5
35	29211.0
36	31904.5
37	34598.0
38	44118.0
39	53638.0
40	53638.0
41	62806.5
42	71975.0
43	76174.0
44	80373.0
45	99101.0
46	117829.0
47	117829.0
48	128457.5
49	139086.0
50	152339.5
51	165593.0
52	169053.5
53	172514.0
54	172514.0
55	150387.5
56	128261.0
57	118183.0
58	108105.0
59	99508.0
60	90911.0
61	90911.0
62	83652.0
63	76393.0
64	64582.5
65	52772.0
66	47017.0
67	41262.0
68	41262.0
69	35774.5
70	30287.0
71	26205.5
72	22124.0
73	18160.5
74	14197.0
75	14197.0
76	11788.0
77	9379.0
78	7743.0
79	6107.0
80	4861.0
81	3615.0
82	3615.0
83	2932.5
84	2250.0
85	1796.0
86	1342.0
87	1054.5
88	767.0
89	767.0
90	565.5
91	364.0
92	229.5
93	95.0
94	53.5
95	12.0
96	12.0
97	7.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1529975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.35162723515984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.37116594734256	38.71721294674851
2	7.251354538384698	6.577214559496149
3	2.2828503205186057	3.1059293030947495
4	1.211092955667345	2.1970014509021363
5	0.7156149431501638	1.6227151072828163
6	0.4795129288169183	1.3048014961286767
7	0.3482956793806411	1.1057043073221307
8	0.2715368518237319	0.9851710467614976
9	0.2140434018910861	0.8736494917239039
>10	1.4638547612176847	13.244110325267048
>50	0.20447299968742402	6.4840089736358815
>100	0.16506481015131158	15.180794226898723
>500	0.01578249968829309	4.9263471748242065
>1k	0.0053573622795123335	3.675339589913635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2768	0.18091798885602706	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2446	0.15987189333159038	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2425	0.1584993218843445	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2389	0.15614634226049445	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2224	0.14536185231784832	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2034	0.13294334874752856	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1961	0.1281720289547215	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1911	0.12490400169937418	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1751	0.11444631448226279	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1737	0.11353126685076553	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1734	0.1133351852154447	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1699	0.11104756613670157	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1674	0.10941355250902791	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1539	0.10058987891959019	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	6.536054510694619E-5	0.0
4	0.0	6.536054510694619E-5	0.0	6.536054510694619E-5	0.0
5	0.0	6.536054510694619E-5	0.0	6.536054510694619E-5	0.0
6	6.536054510694619E-5	6.536054510694619E-5	0.0	6.536054510694619E-5	0.0
7	6.536054510694619E-5	6.536054510694619E-5	0.0	6.536054510694619E-5	0.0
8	1.3072109021389238E-4	6.536054510694619E-5	0.0	6.536054510694619E-5	0.0
9	1.3072109021389238E-4	6.536054510694619E-5	0.0	6.536054510694619E-5	0.0
10	1.3072109021389238E-4	6.536054510694619E-5	0.0	6.536054510694619E-5	0.0
11	1.3072109021389238E-4	6.536054510694619E-5	0.0	1.3072109021389238E-4	0.0
12	1.3072109021389238E-4	6.536054510694619E-5	0.0	1.9608163532083858E-4	0.0
13	1.3072109021389238E-4	6.536054510694619E-5	0.0	1.9608163532083858E-4	0.0
14	1.3072109021389238E-4	6.536054510694619E-5	0.0	3.2680272553473093E-4	0.0
15	1.9608163532083858E-4	6.536054510694619E-5	0.0	5.228843608555695E-4	0.0
16	1.9608163532083858E-4	1.3072109021389238E-4	0.0	0.0012418503570319776	0.0
17	2.6144218042778477E-4	1.3072109021389238E-4	0.0	0.0018954558081014396	0.0
18	3.2680272553473093E-4	1.3072109021389238E-4	0.0	0.002352979623850063	0.0
19	3.2680272553473093E-4	1.3072109021389238E-4	0.0	0.003071945620026471	0.0
20	3.2680272553473093E-4	1.3072109021389238E-4	0.0	0.00411771434173761	0.0
21	3.2680272553473093E-4	1.3072109021389238E-4	0.0	0.006928217781336296	0.0
22	3.2680272553473093E-4	1.3072109021389238E-4	0.0	0.009869442311148875	0.0
23	3.2680272553473093E-4	1.3072109021389238E-4	0.0	0.01098057157796696	0.0
24	3.2680272553473093E-4	1.3072109021389238E-4	0.0	0.014052517197993431	0.0
25	3.2680272553473093E-4	1.3072109021389238E-4	0.0	0.015751891370774034	0.0
26	3.2680272553473093E-4	1.3072109021389238E-4	0.0	0.01856239481037272	0.0
27	3.2680272553473093E-4	1.3072109021389238E-4	0.0	0.037190150165852386	0.0
28	3.2680272553473093E-4	1.9608163532083858E-4	0.0	0.122681743165738	0.0
29	3.2680272553473093E-4	1.9608163532083858E-4	0.0	0.25575581300348044	0.0
30	3.9216327064167715E-4	1.9608163532083858E-4	0.0	0.43033382898413375	0.0
31	3.9216327064167715E-4	1.9608163532083858E-4	0.0	0.9928920407196196	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1610	0.0	30.10559	1
GTACTAG	25	0.0054969057	29.599998	6
TTACTCG	85	5.183574E-8	21.764706	29
TATCCGG	100	1.290573E-8	20.349998	10
AACCGTA	55	5.144164E-4	20.181818	7
CTTATAC	1585	0.0	20.075708	37
GTACTAT	65	6.904038E-5	19.923077	1
ATACGGC	130	3.274181E-11	19.923077	29
GCGGTAA	1040	0.0	19.745192	23
AAACACG	85	1.2458604E-6	19.588236	15
CTATCCG	105	2.260822E-8	19.38095	9
CCGACGT	105	2.260822E-8	19.38095	23
GCAGTCG	415	0.0	19.168674	9
TTCTGCG	810	0.0	18.95679	18
CAGTCGG	420	0.0	18.940475	10
CGCGGTA	1120	0.0	18.5	22
TTAGACT	60	9.2387205E-4	18.5	4
AAGACCG	70	1.2195326E-4	18.5	5
TTATATT	90	2.153165E-6	18.5	4
AGTCGGT	430	0.0	18.5	11
>>END_MODULE