FastQCFastQC Report
Fri 10 Feb 2017
ERR1631690.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631690.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870728
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA18870.2167152084232964No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA18250.20959472992714145No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14750.16939848035207322No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14450.16595308753135307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14400.1653788553945664No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13820.1587177626078408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12750.14642919488060566No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12360.14195018421366948No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11860.13620786284580258No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11780.1352890914269439No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG11620.13345154858922648No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10810.12414898797328212No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10810.12414898797328212No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10390.11932543802427394No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10150.11656912376769783No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9680.11117134168190297No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT9670.11105649525454563No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA9310.10692202386968146No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9280.10657748458760945No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC8810.10117970250181459No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC8810.10117970250181459No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA8790.1009500096470999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.031.821
GTCTAGT250.005495621429.59999813
TTTTACG659.386349E-825.6153854
AACGACC1103.6379788E-1223.54545437
ATACGGC1155.456968E-1222.5217429
TCCGTTT603.7249498E-521.58333431
CCGCTTA957.1395334E-921.42105325
GCGACTT450.00382507420.55555513
GGACCGT1351.8189894E-1220.5555556
TATGTAC555.141719E-420.1818183
GTATCAA19950.019.7518792
GCAGTCG2650.019.547179
GCGGTAA8450.019.26627323
AGTCGGT2850.018.8245611
CGCGGTA8900.018.522
CTATCTA701.21876656E-418.520
TGTTACG609.234362E-418.516
TTACAGT609.234362E-418.54
AACACTA609.234362E-418.55
CGTGAAT500.00703354718.49999818
Produced by FastQC (version 0.11.5)