FastQCFastQC Report
Fri 10 Feb 2017
ERR1631689.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631689.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375649
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9950.26487492313303107No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9280.24703912428889735No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8770.2334626206911239No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8000.21296476231801495No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7420.19752481704995886No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7290.19406413966229114No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7260.19326552180359857No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7190.19140208013331594No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6310.1679759562783343No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6190.16478148484356409No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6020.16025598364430627No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5980.15919115983271617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5860.15599668839794595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.15439945268056085No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5790.15413324672766332No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4890.13017471096688665No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4630.12325335619155116No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4320.11500097165172808No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4310.11473476569883055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4290.11420235379303552No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4210.11207270616985537No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4130.10994305854667522No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4110.10941064664088018No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4060.1080796168763926No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3800.1011582621010571No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3790.10089205614815958No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCC250.005491692629.5999984
GGTATCA5850.026.880341
GCGGTAA3950.022.01265723
CGCGGTA4250.021.32941222
AGCCGCG4100.021.20731519
TAACCAG450.00382108620.5555555
GCTCGGA450.00382108620.55555511
CGTGCCA4350.020.41379410
CCGCGGT4550.020.3296721
CGGTAAT4300.020.2209324
CATTGGC555.1342376E-420.18181833
AACGACC656.8863315E-519.92307737
GGTAATA4400.019.76136425
GACCGTT851.2407472E-619.5882347
CTTATAC4550.019.51648337
TAATACG4000.019.42527
GTATCAA8150.019.0674862
AACTCCG4650.018.6989255
GTGCCAG4750.018.69473611
TAGCGAA609.221042E-418.510
Produced by FastQC (version 0.11.5)