##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631689.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	375649
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42265252935586	33.0	31.0	34.0	31.0	34.0
2	32.600901373356514	34.0	31.0	34.0	31.0	34.0
3	32.68753810072701	34.0	31.0	34.0	31.0	34.0
4	36.16558010270226	37.0	35.0	37.0	35.0	37.0
5	36.061009080285054	37.0	35.0	37.0	35.0	37.0
6	36.11492909604445	37.0	35.0	37.0	35.0	37.0
7	36.110667138738556	37.0	35.0	37.0	35.0	37.0
8	36.08894739504165	37.0	35.0	37.0	35.0	37.0
9	37.911065915255996	39.0	38.0	39.0	35.0	39.0
10	37.81818665829005	39.0	38.0	39.0	35.0	39.0
11	37.89482469006972	39.0	38.0	39.0	35.0	39.0
12	37.82327651610945	39.0	38.0	39.0	35.0	39.0
13	37.8840646454536	39.0	38.0	39.0	35.0	39.0
14	39.21595159310952	40.0	39.0	41.0	36.0	41.0
15	39.22447018360224	40.0	39.0	41.0	36.0	41.0
16	39.15717065665022	40.0	39.0	41.0	36.0	41.0
17	39.12672201975781	40.0	39.0	41.0	36.0	41.0
18	39.16752340615841	40.0	39.0	41.0	36.0	41.0
19	39.18410005084534	40.0	39.0	41.0	36.0	41.0
20	39.172834214918716	40.0	39.0	41.0	36.0	41.0
21	39.14161890488195	40.0	39.0	41.0	36.0	41.0
22	39.10437403001206	40.0	39.0	41.0	36.0	41.0
23	39.0430242061073	40.0	39.0	41.0	36.0	41.0
24	39.05211780145827	40.0	39.0	41.0	36.0	41.0
25	38.99036600656464	40.0	39.0	41.0	35.0	41.0
26	38.91486733626337	40.0	38.0	41.0	35.0	41.0
27	38.79760893813108	40.0	38.0	41.0	35.0	41.0
28	38.785422029607425	40.0	38.0	41.0	35.0	41.0
29	38.76343607995762	40.0	38.0	41.0	35.0	41.0
30	38.725413883705265	40.0	38.0	41.0	35.0	41.0
31	38.629638305971795	40.0	38.0	41.0	35.0	41.0
32	38.59812484526779	40.0	38.0	41.0	35.0	41.0
33	38.522724671169094	40.0	38.0	41.0	35.0	41.0
34	38.4992692646593	40.0	38.0	41.0	35.0	41.0
35	38.42615579969599	40.0	38.0	41.0	34.0	41.0
36	38.37797518428107	40.0	38.0	41.0	34.0	41.0
37	38.321334543683065	40.0	38.0	41.0	34.0	41.0
38	38.282809218179736	40.0	38.0	41.0	34.0	41.0
39	38.21523017497717	40.0	38.0	41.0	34.0	41.0
40	37.90436551142157	40.0	37.0	41.0	33.0	41.0
41	37.96619184398202	40.0	37.0	41.0	33.0	41.0
42	37.94947411014005	40.0	37.0	41.0	33.0	41.0
43	37.45599482495628	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	8.0
20	18.0
21	36.0
22	73.0
23	151.0
24	246.0
25	419.0
26	663.0
27	968.0
28	1470.0
29	2179.0
30	3067.0
31	4199.0
32	5552.0
33	7585.0
34	10981.0
35	16148.0
36	24602.0
37	44394.0
38	102486.0
39	150400.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.64113574107744	16.445405152150013	13.051545458659547	27.861913648113003
2	20.9208596322631	19.579980247518293	32.64217394429374	26.856986175924867
3	21.151393987472346	19.987541561404395	29.66625759685238	29.194806854270876
4	15.439146650197392	16.333864857885953	34.541021006311745	33.68596748560491
5	16.869737441068658	32.88575239119497	33.65987930222096	16.584630865515415
6	33.12746739642592	34.18510364728776	15.923641484470878	16.763787471815444
7	29.805483310217785	27.96174088044957	20.293412201283644	21.939363608049003
8	27.115738362141258	30.79390601332627	20.696980425876284	21.39337519865619
9	28.356790514549484	12.856682701138563	17.973959733687565	40.81256705062439
10	19.26186413380576	23.436772093097545	29.848076262681385	27.453287510415308
11	38.592941815364874	19.755676176430654	19.10639985731361	22.544982150890856
12	22.482956163865737	24.136893749218018	26.99887394881924	26.381276138097
13	34.94192717137541	17.934028840752937	21.12770165766447	25.996342330207188
14	23.84726167246552	20.5662733030036	23.73359173057828	31.852873293952598
15	29.538744945414468	24.344268186525188	20.47842533854742	25.63856152951292
16	25.032410574765272	24.433180974792958	23.121318038913984	27.413090411527786
17	26.493615050219756	24.052506462149505	22.3043319694715	27.14954651815924
18	26.381276138097	21.656919092024733	23.843268583172055	28.118536186706205
19	27.712305902584593	22.61206605102103	23.972910882233148	25.702717164161225
20	29.667056214711074	20.96105673115062	23.359838572710164	26.012048481428142
21	27.09816876925002	22.46006245191655	22.458465216199166	27.983303562634266
22	28.010988981735608	23.091236766236566	21.95666699498734	26.941107257040482
23	27.758891944341656	22.261206605102103	23.053169314972223	26.926732135584015
24	27.37821743169821	21.896770655585403	23.654794768520617	27.07021714419578
25	27.572813983266293	22.240974952681892	23.755154412762977	26.431056651288838
26	27.34866857092658	23.0765954388272	22.967184792186323	26.60755119805989
27	26.5423307396	22.07273279045066	24.702847605078144	26.682088864871194
28	25.870160708533763	23.331620741703027	22.781639243016752	28.016579306746458
29	26.978376090446133	23.65692441614379	22.911015336124947	26.453684157285124
30	26.582261632534625	22.30113749803673	24.338677861514338	26.7779230079143
31	27.44210686039361	22.791222657321065	23.40748943827882	26.359181044006508
32	25.386996904024766	22.574531011662483	23.613532845821496	28.424939238491255
33	25.5874499865566	22.037859810621086	25.28823449549979	27.086455707322525
34	25.822509842965108	22.121714685783804	24.651203650216026	27.404571821035063
35	25.374485224238587	23.599423930317933	25.19506241198566	25.83102843345783
36	25.646813914052746	22.45287489118832	25.545655651951687	26.354655542807247
37	26.3386831856334	22.190395821631366	24.932583342428703	26.538337650306538
38	25.424798149336215	21.68007900992682	25.97158517658772	26.923537664149244
39	24.900106216175207	20.447545448011308	27.17323884796712	27.479109487846365
40	24.57746460126341	21.669963183716714	27.712572108537493	26.04000010648238
41	22.753155206056718	20.87640323812921	28.946969112123284	27.423472443690788
42	21.480158339300782	21.391245551033013	29.626060497964858	27.50253561170135
43	20.78536080223826	22.116124360772957	29.341486334317405	27.75702850267138
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.0
2	1.0
3	10.0
4	19.0
5	19.0
6	33.5
7	48.0
8	46.0
9	44.0
10	84.5
11	125.0
12	125.0
13	240.0
14	355.0
15	695.0
16	1035.0
17	989.0
18	943.0
19	943.0
20	1096.5
21	1250.0
22	1042.5
23	835.0
24	915.5
25	996.0
26	996.0
27	1152.5
28	1309.0
29	1727.0
30	2145.0
31	2610.0
32	3075.0
33	3075.0
34	3858.0
35	4641.0
36	5254.5
37	5868.0
38	7438.0
39	9008.0
40	9008.0
41	10782.5
42	12557.0
43	14599.5
44	16642.0
45	22975.0
46	29308.0
47	29308.0
48	33136.0
49	36964.0
50	41116.5
51	45269.0
52	45524.0
53	45779.0
54	45779.0
55	41174.5
56	36570.0
57	34245.5
58	31921.0
59	29051.0
60	26181.0
61	26181.0
62	23627.5
63	21074.0
64	17280.5
65	13487.0
66	11601.0
67	9715.0
68	9715.0
69	8166.5
70	6618.0
71	5651.5
72	4685.0
73	3735.0
74	2785.0
75	2785.0
76	2260.0
77	1735.0
78	1416.0
79	1097.0
80	884.0
81	671.0
82	671.0
83	544.5
84	418.0
85	326.5
86	235.0
87	178.5
88	122.0
89	122.0
90	103.5
91	85.0
92	55.0
93	25.0
94	13.5
95	2.0
96	2.0
97	3.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	375649.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.71129789722896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.51760630823686	43.87420106852175
2	6.089653352498203	6.176284504987909
3	2.087648057202311	3.17602027600073
4	1.1531476043984505	2.3391044674450305
5	0.7451186062813849	1.8892965805951685
6	0.49900863976863685	1.5183225470759016
7	0.3629991267002804	1.2885709795372315
8	0.2874385581296648	1.1661105878770717
9	0.21527289314226167	0.9825091032002014
>10	1.742542916909386	17.398287908428717
>50	0.17615203321948547	6.184026135197149
>100	0.11550088405708774	11.142587009972473
>500	0.007911019455964913	2.8646788311606537
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	995	0.26487492313303107	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	928	0.24703912428889735	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	877	0.2334626206911239	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	800	0.21296476231801495	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	742	0.19752481704995886	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	729	0.19406413966229114	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	726	0.19326552180359857	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	719	0.19140208013331594	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	631	0.1679759562783343	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	619	0.16478148484356409	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	602	0.16025598364430627	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	598	0.15919115983271617	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	586	0.15599668839794595	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	580	0.15439945268056085	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	579	0.15413324672766332	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	489	0.13017471096688665	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	463	0.12325335619155116	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	432	0.11500097165172808	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	431	0.11473476569883055	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	429	0.11420235379303552	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	421	0.11207270616985537	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	413	0.10994305854667522	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	411	0.10941064664088018	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	406	0.1080796168763926	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	380	0.1011582621010571	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	379	0.10089205614815958	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	2.662059528975187E-4	0.0	0.0	0.0	0.0
14	2.662059528975187E-4	0.0	0.0	0.0	0.0
15	2.662059528975187E-4	0.0	0.0	0.0	0.0
16	2.662059528975187E-4	0.0	0.0	5.324119057950374E-4	0.0
17	2.662059528975187E-4	0.0	0.0	0.0021296476231801495	0.0
18	2.662059528975187E-4	0.0	0.0	0.0029282654818727056	0.0
19	2.662059528975187E-4	0.0	0.0	0.003460677387667743	0.0
20	2.662059528975187E-4	0.0	0.0	0.004525501199257818	0.0
21	2.662059528975187E-4	0.0	0.0	0.009583414304310672	0.0
22	2.662059528975187E-4	0.0	0.0	0.014641327409363528	0.0
23	5.324119057950374E-4	0.0	0.0	0.01836821074992879	0.0
24	5.324119057950374E-4	0.0	0.0	0.021296476231801496	0.0
25	5.324119057950374E-4	0.0	0.0	0.023426123854981645	0.0
26	7.986178586925561E-4	0.0	0.0	0.026354389336854352	0.0
27	7.986178586925561E-4	0.0	0.0	0.04445639413388562	0.0
28	7.986178586925561E-4	0.0	0.0	0.13895950741250476	0.0
29	7.986178586925561E-4	0.0	0.0	0.2890996648467053	0.0
30	7.986178586925561E-4	0.0	0.0	0.49540927834228227	0.0
31	7.986178586925561E-4	0.0	0.0	1.1082153819123703	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTCC	25	0.0054916926	29.599998	4
GGTATCA	585	0.0	26.88034	1
GCGGTAA	395	0.0	22.012657	23
CGCGGTA	425	0.0	21.329412	22
AGCCGCG	410	0.0	21.207315	19
TAACCAG	45	0.003821086	20.555555	5
GCTCGGA	45	0.003821086	20.555555	11
CGTGCCA	435	0.0	20.413794	10
CCGCGGT	455	0.0	20.32967	21
CGGTAAT	430	0.0	20.22093	24
CATTGGC	55	5.1342376E-4	20.181818	33
AACGACC	65	6.8863315E-5	19.923077	37
GGTAATA	440	0.0	19.761364	25
GACCGTT	85	1.2407472E-6	19.588234	7
CTTATAC	455	0.0	19.516483	37
TAATACG	400	0.0	19.425	27
GTATCAA	815	0.0	19.067486	2
AACTCCG	465	0.0	18.698925	5
GTGCCAG	475	0.0	18.694736	11
TAGCGAA	60	9.221042E-4	18.5	10
>>END_MODULE