FastQCFastQC Report
Fri 10 Feb 2017
ERR1631688.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631688.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466842
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10190.2182751337711688No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9400.20135292025995946No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9060.19406994229310986No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8490.18186024393692085No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7890.1690079298777745No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7770.16643746706594523No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7610.16301018331683953No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7340.15722664199022368No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6700.14351750699380092No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6560.14051863371333342No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6350.1360203237926322No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6300.1349492976210367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.12980837199737813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5970.12788052488850618No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5890.12616688301395335No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4990.1068884119252338No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4820.10324692294180901No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4690.10046225489566063No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4670.10003384442702243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7450.026.5704691
ATACGGC451.3215678E-424.66666829
GCGGTAA3550.022.92957723
CGCGGTA3650.022.3013722
TTATGAG450.003822455220.5555573
CGGTAAC450.003822455220.55555722
GGTAATA4000.020.34999825
CCGCGGT4050.020.09876421
AGCCGCG4200.019.82142819
AACTCCG4200.019.8214285
CGGTAAT4150.019.61445824
CGTGCCA4250.019.58823610
TAATACG4000.018.962527
TACGGCT609.22562E-418.530
CGTTAGA801.613321E-518.52
GTGCCAG4500.018.511
ACTATAC500.00702877818.4999983
TACCCTC500.00702877818.4999985
GTAATAC4400.018.49999826
CGTGTAG500.00702877818.4999988
Produced by FastQC (version 0.11.5)