##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631688.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	466842
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.412561423350944	33.0	31.0	34.0	31.0	34.0
2	32.59335064111627	34.0	31.0	34.0	31.0	34.0
3	32.68246001859301	34.0	31.0	34.0	31.0	34.0
4	36.16715719665326	37.0	35.0	37.0	35.0	37.0
5	36.05657160238368	37.0	35.0	37.0	35.0	37.0
6	36.107822346746865	37.0	35.0	37.0	35.0	37.0
7	36.107001940699426	37.0	35.0	37.0	35.0	37.0
8	36.08190351339425	37.0	35.0	37.0	35.0	37.0
9	37.88495679480424	39.0	38.0	39.0	35.0	39.0
10	37.79544042738228	39.0	37.0	39.0	35.0	39.0
11	37.88443199198016	39.0	38.0	39.0	35.0	39.0
12	37.80404290959254	39.0	38.0	39.0	35.0	39.0
13	37.87092420990399	39.0	38.0	39.0	35.0	39.0
14	39.20100376572802	40.0	39.0	41.0	36.0	41.0
15	39.20313082370481	40.0	39.0	41.0	36.0	41.0
16	39.138562939924	40.0	39.0	41.0	36.0	41.0
17	39.11578649735885	40.0	39.0	41.0	36.0	41.0
18	39.147565985922434	40.0	39.0	41.0	36.0	41.0
19	39.167202179752465	40.0	39.0	41.0	36.0	41.0
20	39.155907137746816	40.0	39.0	41.0	36.0	41.0
21	39.114576237784945	40.0	39.0	41.0	36.0	41.0
22	39.091304981128516	40.0	39.0	41.0	36.0	41.0
23	39.03452988377224	40.0	39.0	41.0	36.0	41.0
24	39.04516303160384	40.0	39.0	41.0	36.0	41.0
25	38.99510969450049	40.0	38.0	41.0	35.0	41.0
26	38.924741132974326	40.0	38.0	41.0	35.0	41.0
27	38.80826275270862	40.0	38.0	41.0	35.0	41.0
28	38.797657451557484	40.0	38.0	41.0	35.0	41.0
29	38.767576610502054	40.0	38.0	41.0	35.0	41.0
30	38.72093127867672	40.0	38.0	41.0	35.0	41.0
31	38.633094280291836	40.0	38.0	41.0	35.0	41.0
32	38.58992121531482	40.0	38.0	41.0	35.0	41.0
33	38.53067204750215	40.0	38.0	41.0	35.0	41.0
34	38.49526820637389	40.0	38.0	41.0	34.0	41.0
35	38.42280685970842	40.0	38.0	41.0	34.0	41.0
36	38.37908757138389	40.0	38.0	41.0	34.0	41.0
37	38.33142476469555	40.0	38.0	41.0	34.0	41.0
38	38.291449783866916	40.0	38.0	41.0	34.0	41.0
39	38.21920692654046	40.0	38.0	41.0	34.0	41.0
40	37.9187091135759	40.0	37.0	41.0	33.0	41.0
41	37.990035172499475	40.0	37.0	41.0	33.0	41.0
42	37.966399766944704	40.0	37.0	41.0	33.0	41.0
43	37.46955715209857	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	3.0
18	5.0
19	11.0
20	34.0
21	50.0
22	105.0
23	165.0
24	317.0
25	484.0
26	814.0
27	1283.0
28	1906.0
29	2754.0
30	3785.0
31	5243.0
32	6822.0
33	9469.0
34	13395.0
35	19857.0
36	31247.0
37	55125.0
38	127080.0
39	186886.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.35415836621384	16.96698240518205	13.11792854970204	27.560930678902068
2	20.442033921540904	19.920229970739562	33.065362585200134	26.572373522519392
3	21.014818718110195	20.442248126775226	29.466714648639154	29.076218506475428
4	15.608707014364603	16.39548284001868	34.168733747177846	33.82707639843887
5	16.69944006751749	33.071574536995385	33.58566709936124	16.643318296125884
6	33.49163100149515	34.078981753998136	15.727805124646027	16.70158211986068
7	29.796590709490577	28.417751616178492	20.333003457272483	21.45265421705845
8	27.000355580688968	31.103028433602802	20.486160199810644	21.410455785897582
9	28.263738052703058	13.243881227481674	18.118978155350206	40.37340256446507
10	18.973014424580477	23.827976060423012	30.305328140998455	26.893681373998056
11	37.98629943321295	20.264029371821728	19.251267023960995	22.49840417100432
12	22.370737851350135	24.407401219256194	27.09010757386868	26.131753355524996
13	34.40500212063182	18.25585530008011	21.465506531117594	25.87363604817047
14	23.734368373025564	20.76248495208229	23.663038029997303	31.840108644894848
15	29.018383093209266	24.805608749855413	20.552778027683885	25.62323012925144
16	25.00160653925739	24.69122315472901	23.036916130082556	27.27025417593104
17	26.1698818872338	24.511290757900962	22.421290286649445	26.897537068215797
18	26.142892027709593	22.018584446129523	24.080738236919558	27.757785289241326
19	27.37885622973083	22.972868765021143	24.061888176299476	25.586386828948555
20	29.198958105740267	21.62016271029599	23.420343499513756	25.760535684449987
21	26.772869621842077	23.130095407011368	22.57337600301601	27.523658968130544
22	27.53908174500152	23.65189935781271	22.09826879329623	26.71075010388954
23	27.19142664970161	22.916318583160898	23.11510104060903	26.777153726528464
24	27.054977915440343	22.51082807459483	23.532587042296964	26.901606967667863
25	27.096533730898248	22.844988240132636	23.66560849280913	26.392869536159985
26	26.991787371316207	23.489317584964507	22.949948804948995	26.56894623877029
27	26.490332917775177	22.532034392792422	24.412542144879854	26.565090544552543
28	25.839149005445094	23.480535170357424	22.83235013130781	27.84796569288967
29	26.42799919458832	24.03896821622733	23.147017620522575	26.386014968661776
30	26.272700399706967	23.029847357350025	24.24760411445414	26.449848128488867
31	27.276037717257655	23.061121321560613	23.24126792362298	26.421573037558744
32	24.949126256849212	23.165010860205378	23.5957775864211	28.290085296524303
33	25.346691171745473	22.51361274264098	25.17918267850793	26.96051340710562
34	25.722407152741184	22.498618376238642	24.670231041765735	27.10874342925444
35	25.346262761276833	23.79713050668106	25.005248028240818	25.851358703801285
36	25.55854014848707	22.701684938373155	25.393816323295678	26.345958589844106
37	26.06406450148016	22.356386100650756	24.9714036012184	26.60814579665069
38	25.24665732731845	21.832011687037582	26.072632710852922	26.848698274791044
39	24.715642551441384	20.77683670278167	27.171291357675614	27.33622938810133
40	24.255529708123948	21.906340903346315	27.796128026184448	26.04200136234529
41	22.790580110615583	21.19774998821871	28.682509285796908	27.329160615368796
42	21.51413111930803	21.70605900925795	29.39816897365704	27.381640897776975
43	20.88972286126784	22.318685979410592	29.264933317910558	27.526657841411012
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	4.0
2	7.0
3	20.0
4	33.0
5	33.0
6	46.0
7	59.0
8	63.5
9	68.0
10	113.5
11	159.0
12	159.0
13	313.5
14	468.0
15	838.0
16	1208.0
17	1192.0
18	1176.0
19	1176.0
20	1326.0
21	1476.0
22	1352.0
23	1228.0
24	1391.0
25	1554.0
26	1554.0
27	1873.0
28	2192.0
29	2888.5
30	3585.0
31	4238.5
32	4892.0
33	4892.0
34	5939.0
35	6986.0
36	7941.5
37	8897.0
38	10816.0
39	12735.0
40	12735.0
41	15212.0
42	17689.0
43	20092.0
44	22495.0
45	29349.0
46	36203.0
47	36203.0
48	39878.5
49	43554.0
50	48646.5
51	53739.0
52	53931.0
53	54123.0
54	54123.0
55	49239.5
56	44356.0
57	41370.0
58	38384.0
59	35218.0
60	32052.0
61	32052.0
62	28989.0
63	25926.0
64	21247.5
65	16569.0
66	14200.0
67	11831.0
68	11831.0
69	9950.5
70	8070.0
71	6923.0
72	5776.0
73	4662.0
74	3548.0
75	3548.0
76	2894.0
77	2240.0
78	1849.5
79	1459.0
80	1154.5
81	850.0
82	850.0
83	704.5
84	559.0
85	452.5
86	346.0
87	263.5
88	181.0
89	181.0
90	149.5
91	118.0
92	81.0
93	44.0
94	24.5
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	466842.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.21329071106437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.55876239838403	46.59289877806221
2	5.736173335185527	6.1048131850856615
3	1.9355969590894049	3.0899845105042996
4	1.0144773491664256	2.159347124039318
5	0.65285587123094	1.7370304634118607
6	0.46889351581340544	1.4970820181707079
7	0.32548703260363476	1.2124165262033229
8	0.24793108317102275	1.0554583044070969
9	0.20900226386325196	1.0009528403603227
>10	1.566917501606687	16.43377971425221
>50	0.17592260698185036	6.395894873702019
>100	0.10191378611150285	10.31908341820665
>500	0.005661877006194602	2.1819642706401927
>1k	4.044197861567573E-4	0.2192939729541726
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1019	0.2182751337711688	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	940	0.20135292025995946	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	906	0.19406994229310986	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	849	0.18186024393692085	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	789	0.1690079298777745	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	777	0.16643746706594523	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	761	0.16301018331683953	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	734	0.15722664199022368	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	670	0.14351750699380092	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	656	0.14051863371333342	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	635	0.1360203237926322	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	630	0.1349492976210367	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	606	0.12980837199737813	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	597	0.12788052488850618	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	589	0.12616688301395335	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	499	0.1068884119252338	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	482	0.10324692294180901	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	469	0.10046225489566063	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	467	0.10003384442702243	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.142052343191058E-4	0.0	0.0	0.0	0.0
6	2.142052343191058E-4	0.0	0.0	0.0	0.0
7	2.142052343191058E-4	0.0	0.0	0.0	0.0
8	2.142052343191058E-4	0.0	0.0	0.0	0.0
9	4.284104686382116E-4	0.0	0.0	0.0	0.0
10	4.284104686382116E-4	0.0	0.0	0.0	0.0
11	4.284104686382116E-4	0.0	0.0	0.0	0.0
12	4.284104686382116E-4	0.0	0.0	0.0	0.0
13	4.284104686382116E-4	0.0	0.0	2.142052343191058E-4	0.0
14	4.284104686382116E-4	0.0	0.0	4.284104686382116E-4	0.0
15	4.284104686382116E-4	0.0	0.0	6.426157029573175E-4	0.0
16	6.426157029573175E-4	0.0	0.0	8.568209372764232E-4	0.0
17	6.426157029573175E-4	0.0	0.0	0.0017136418745528465	0.0
18	8.568209372764232E-4	0.0	0.0	0.0021420523431910582	0.0
19	0.0010710261715955291	0.0	0.0	0.0027846680461483756	0.0
20	0.0010710261715955291	0.0	0.0	0.0029988732804674817	0.0
21	0.001285231405914635	0.0	0.0	0.0044983099207012225	0.0
22	0.001285231405914635	0.0	0.0	0.006426157029573175	0.0
23	0.001285231405914635	0.0	0.0	0.007282977966849598	0.0
24	0.001285231405914635	0.0	0.0	0.011138672184593502	0.0
25	0.001285231405914635	0.0	0.0	0.012638108824827243	0.0
26	0.001285231405914635	0.0	0.0	0.01649380304257115	0.0
27	0.001285231405914635	0.0	0.0	0.03491545319401425	0.0
28	0.0014994366402337408	0.0	0.0	0.12937996152873993	0.0
29	0.0014994366402337408	0.0	0.0	0.2767531627402847	0.0
30	0.0014994366402337408	0.0	0.0	0.4744645940168194	0.0
31	0.0014994366402337408	0.0	0.0	1.0675988878464233	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	745	0.0	26.570469	1
ATACGGC	45	1.3215678E-4	24.666668	29
GCGGTAA	355	0.0	22.929577	23
CGCGGTA	365	0.0	22.30137	22
TTATGAG	45	0.0038224552	20.555557	3
CGGTAAC	45	0.0038224552	20.555557	22
GGTAATA	400	0.0	20.349998	25
CCGCGGT	405	0.0	20.098764	21
AGCCGCG	420	0.0	19.821428	19
AACTCCG	420	0.0	19.821428	5
CGGTAAT	415	0.0	19.614458	24
CGTGCCA	425	0.0	19.588236	10
TAATACG	400	0.0	18.9625	27
TACGGCT	60	9.22562E-4	18.5	30
CGTTAGA	80	1.613321E-5	18.5	2
GTGCCAG	450	0.0	18.5	11
ACTATAC	50	0.007028778	18.499998	3
TACCCTC	50	0.007028778	18.499998	5
GTAATAC	440	0.0	18.499998	26
CGTGTAG	50	0.007028778	18.499998	8
>>END_MODULE