FastQCFastQC Report
Fri 10 Feb 2017
ERR1631687.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631687.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316482
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53440.40593035073779965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48750.37030510101923153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41000.311436084959764No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA27750.21078905750325488No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA24440.1856462906443081No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA23700.18002524911088794No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA22380.16999852637559798No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA19850.15078064113295891No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19250.1462230398896453No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG19230.14607111984820148No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT17660.13414539659486419No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC16810.12768879483350323No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC16800.12761283481278132No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA16520.1254859542325683No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC15850.12039663284420143No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT15570.11826975226398842No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT15360.11667459182882864No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA14580.11074971021252093No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC14530.11036991010891148No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13930.10581230886559786No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13740.10436906847188189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG303.5997608E-430.8333325
GGTATCA22650.028.1788081
TGTCGCA450.003826098720.55555531
CGAACTA1052.2600943E-819.38095324
GCGGTAA9050.018.8066323
CTTATAC15800.018.61708837
ATACGGC1103.8528015E-818.529
CGAGAAC500.007035413318.4999983
TACAGCG500.007035413318.4999985
TATACAC4800.018.11458237
TGCGGGT8400.018.05952521
CGCGGTA9650.018.02072522
GTATCAA35650.017.7475472
GTAGTAA1054.798567E-717.619051
TTTTACG852.7236018E-517.4117644
AGCCGCG10100.017.40099119
TTCTGCG8650.017.323718
TAGTACC752.0675545E-417.2666664
GGACGTA1401.869921E-917.17857227
CGTGCCA10600.017.10377310
Produced by FastQC (version 0.11.5)