##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631687.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1316482
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21047762141829	33.0	31.0	34.0	30.0	34.0
2	32.39221880739729	34.0	31.0	34.0	31.0	34.0
3	32.47728415580312	34.0	31.0	34.0	31.0	34.0
4	36.04188815342709	37.0	35.0	37.0	35.0	37.0
5	35.87439326933448	37.0	35.0	37.0	35.0	37.0
6	35.91837564053287	37.0	35.0	37.0	35.0	37.0
7	35.91768288514389	37.0	35.0	37.0	35.0	37.0
8	35.88903076532759	37.0	35.0	37.0	35.0	37.0
9	37.666922905136566	39.0	37.0	39.0	35.0	39.0
10	37.554384336436044	39.0	37.0	39.0	35.0	39.0
11	37.64082835921798	39.0	37.0	39.0	35.0	39.0
12	37.559526829838916	39.0	37.0	39.0	35.0	39.0
13	37.631883307177766	39.0	37.0	39.0	35.0	39.0
14	38.875697502890276	40.0	38.0	41.0	36.0	41.0
15	38.867433812235944	40.0	38.0	41.0	36.0	41.0
16	38.80611888350923	40.0	38.0	41.0	36.0	41.0
17	38.787083302316326	40.0	38.0	41.0	35.0	41.0
18	38.82093944315228	40.0	38.0	41.0	35.0	41.0
19	38.85176705796205	40.0	38.0	41.0	35.0	41.0
20	38.834767205324496	40.0	38.0	41.0	35.0	41.0
21	38.79735157791751	40.0	38.0	41.0	35.0	41.0
22	38.761354883697614	40.0	38.0	41.0	35.0	41.0
23	38.69668556045582	40.0	38.0	41.0	35.0	41.0
24	38.70113985607095	40.0	38.0	41.0	35.0	41.0
25	38.640325503880796	40.0	38.0	41.0	35.0	41.0
26	38.549837369595636	40.0	38.0	41.0	35.0	41.0
27	38.42085345640882	40.0	38.0	41.0	34.0	41.0
28	38.404232644274664	40.0	38.0	41.0	34.0	41.0
29	38.359996566607066	40.0	38.0	41.0	34.0	41.0
30	38.29386121496534	40.0	38.0	41.0	34.0	41.0
31	38.18968052734485	40.0	38.0	41.0	34.0	41.0
32	38.12210953131148	40.0	38.0	41.0	34.0	41.0
33	38.03302893621029	40.0	37.0	41.0	33.0	41.0
34	37.99108837036891	40.0	37.0	41.0	33.0	41.0
35	37.89505591417125	40.0	37.0	41.0	33.0	41.0
36	37.83703309274263	40.0	37.0	41.0	33.0	41.0
37	37.774218713206864	40.0	37.0	41.0	33.0	41.0
38	37.73078401375788	40.0	37.0	41.0	33.0	41.0
39	37.641721649061665	40.0	37.0	41.0	33.0	41.0
40	37.318087144374175	40.0	36.0	41.0	32.0	41.0
41	37.354516810712184	39.0	36.0	41.0	32.0	41.0
42	37.302859439020054	39.0	36.0	41.0	32.0	41.0
43	36.7814827699885	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	0.0
14	0.0
15	2.0
16	6.0
17	4.0
18	17.0
19	62.0
20	79.0
21	204.0
22	390.0
23	742.0
24	1161.0
25	1945.0
26	3022.0
27	4718.0
28	7097.0
29	9950.0
30	14063.0
31	18362.0
32	24158.0
33	32928.0
34	46550.0
35	68331.0
36	107759.0
37	198466.0
38	399274.0
39	377190.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.38683096312749	17.604950162630402	13.562737659914834	26.445481214327277
2	20.02116246177312	20.721741732891143	33.97517018842643	25.281925616909305
3	20.895690180344282	21.253689758006566	30.24469761075351	27.605922450895644
4	15.11095480226847	17.190132489468144	34.85790158923555	32.84101111902783
5	16.115222236232626	33.74516324568053	33.83889791125135	16.30071660683549
6	31.940581033390508	35.1599186316258	16.2552925144438	16.644207820539894
7	28.763553166697303	29.352851007457755	20.814792758275463	21.068803067569476
8	26.41251456533397	31.934732111794922	20.62367734613918	21.029075976731924
9	27.838132234242472	13.851537658699472	18.850086822303684	39.46024328475437
10	18.92566704292197	24.587347187428314	30.44158598446466	26.045399785185065
11	37.01607769798599	20.931239470042126	20.080259357894754	21.972423474077125
12	21.957839150098522	25.11990289270951	27.921156536891505	25.00110142030047
13	33.82150306650604	18.772607601167355	22.298595803056937	25.107293529269676
14	23.247564341935554	21.29159380834679	25.125751814305097	30.33509003541256
15	28.30004512025231	25.379306363474775	21.765204537547795	24.555443978725116
16	23.909935722630465	25.36532971966195	24.239754132604926	26.484980425102663
17	25.050627353811144	25.03133350854778	23.833823781867128	26.08421535577395
18	25.248731087853844	22.555340673096936	25.48177643142861	26.714151807620613
19	26.617606621283084	23.579357712448783	25.697275010216625	24.105760656051505
20	27.983519713904176	22.1735656089487	25.059058916111272	24.783855761035852
21	25.903430506455845	23.653190852590463	24.255705736956525	26.187672903997168
22	26.365115512403513	24.229499529807473	23.827974860271542	25.577410097517472
23	26.114219563959097	23.38634329979445	24.871969385073246	25.627467751173207
24	25.918698470620942	23.21983893437206	25.240527405615875	25.62093518939112
25	26.01737053753868	23.432223152310474	25.418501734167272	25.131904575983565
26	25.860285214685806	24.093758972777447	24.84561125788275	25.200344554654
27	25.525681323405863	23.297697955612	25.956374640899	25.22024608008313
28	25.020015465460222	24.331893637740585	24.47735707742301	26.170733819376185
29	25.33980715269939	24.779070279730373	24.634290480234444	25.24683208733579
30	25.193887952892634	23.687372861915318	25.9508295593863	25.167909625805745
31	26.035373062449775	23.69907070510649	24.982795055306493	25.282761177137246
32	23.858510788601745	23.72565671235915	25.422299735203367	26.993532763835738
33	24.13963882529347	23.39500274215675	26.810469113895973	25.654889318653808
34	24.616971595509852	23.308256398492347	26.206890789239807	25.867881216757997
35	24.374431249344845	24.242108893247305	26.458774217953607	24.924685639454243
36	24.512754447079413	23.289722153436205	26.891594416026958	25.305928983457427
37	24.86756370387138	22.98626187065224	26.364431872217015	25.781742553259367
38	24.149589588008038	22.568253876619657	27.294182525852996	25.98797400951931
39	23.67597885880703	21.759887336097268	28.29563943905044	26.268494366045264
40	23.42569059052839	22.59111784285695	28.70582355094867	25.277368015665996
41	22.081274183771598	22.106948670775598	29.615976519238394	26.19580062621441
42	20.857254409859003	22.680826627329502	30.001169784319114	26.460749178492378
43	20.318925743002943	23.259869865292497	29.55589214284738	26.865312248857183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	65.0
1	61.0
2	57.0
3	144.5
4	232.0
5	232.0
6	337.0
7	442.0
8	490.0
9	538.0
10	845.0
11	1152.0
12	1152.0
13	2274.0
14	3396.0
15	6555.0
16	9714.0
17	9442.5
18	9171.0
19	9171.0
20	10242.5
21	11314.0
22	9590.5
23	7867.0
24	8228.5
25	8590.0
26	8590.0
27	9538.5
28	10487.0
29	12827.5
30	15168.0
31	17288.0
32	19408.0
33	19408.0
34	23181.5
35	26955.0
36	29288.5
37	31622.0
38	37233.0
39	42844.0
40	42844.0
41	49754.5
42	56665.0
43	63182.0
44	69699.0
45	87095.5
46	104492.0
47	104492.0
48	113672.5
49	122853.0
50	134623.5
51	146394.0
52	144946.5
53	143499.0
54	143499.0
55	128003.5
56	112508.0
57	103813.0
58	95118.0
59	86191.5
60	77265.0
61	77265.0
62	69830.0
63	62395.0
64	51058.5
65	39722.0
66	34182.0
67	28642.0
68	28642.0
69	24364.5
70	20087.0
71	17124.0
72	14161.0
73	11634.5
74	9108.0
75	9108.0
76	7494.0
77	5880.0
78	4751.0
79	3622.0
80	2922.0
81	2222.0
82	2222.0
83	1784.5
84	1347.0
85	1121.0
86	895.0
87	703.5
88	512.0
89	512.0
90	392.5
91	273.0
92	178.5
93	84.0
94	48.5
95	13.0
96	13.0
97	8.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1316482.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.36660004461615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.97891473160169	40.329165516749484
2	6.337752370237151	5.877200586652081
3	2.0074998251243197	2.792428244435286
4	1.0079027513304795	1.8693209501923422
5	0.6433087905827559	1.491402069906819
6	0.4427150482754259	1.2316314946271765
7	0.3135702492727019	1.0177430433742574
8	0.24118435241342615	0.8946318724298467
9	0.1935846156958515	0.8078274405684243
>10	1.453999209910907	13.733810371156977
>50	0.209449509618826	6.787668705530918
>100	0.14973719530969767	13.461571770451119
>500	0.013149258468935499	4.127958038280238
>1k	0.00706772642705283	5.170369374892388
>5k	1.6436573086169373E-4	0.40727052075269254
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5344	0.40593035073779965	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4875	0.37030510101923153	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4100	0.311436084959764	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2775	0.21078905750325488	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2444	0.1856462906443081	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2370	0.18002524911088794	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2238	0.16999852637559798	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1985	0.15078064113295891	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1925	0.1462230398896453	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1923	0.14607111984820148	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1766	0.13414539659486419	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1681	0.12768879483350323	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1680	0.12761283481278132	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1652	0.1254859542325683	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1585	0.12039663284420143	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	1557	0.11826975226398842	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1536	0.11667459182882864	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1458	0.11074971021252093	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	1453	0.11036991010891148	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1393	0.10581230886559786	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	1374	0.10436906847188189	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	7.596002072189365E-5	0.0	0.0	0.0
5	0.0	7.596002072189365E-5	0.0	0.0	0.0
6	0.0	7.596002072189365E-5	0.0	0.0	0.0
7	0.0	7.596002072189365E-5	0.0	0.0	0.0
8	7.596002072189365E-5	7.596002072189365E-5	0.0	0.0	0.0
9	7.596002072189365E-5	7.596002072189365E-5	0.0	0.0	0.0
10	7.596002072189365E-5	7.596002072189365E-5	0.0	0.0	0.0
11	7.596002072189365E-5	7.596002072189365E-5	0.0	2.2788006216568096E-4	0.0
12	7.596002072189365E-5	7.596002072189365E-5	0.0	3.7980010360946824E-4	0.0
13	7.596002072189365E-5	7.596002072189365E-5	0.0	3.7980010360946824E-4	0.0
14	7.596002072189365E-5	7.596002072189365E-5	0.0	4.557601243313619E-4	0.0
15	1.519200414437873E-4	7.596002072189365E-5	0.0	7.596002072189365E-4	0.0
16	1.519200414437873E-4	7.596002072189365E-5	0.0	0.0011394003108284047	7.596002072189365E-5
17	1.519200414437873E-4	7.596002072189365E-5	0.0	0.001519200414437873	7.596002072189365E-5
18	1.519200414437873E-4	7.596002072189365E-5	0.0	0.001974960538769235	7.596002072189365E-5
19	1.519200414437873E-4	7.596002072189365E-5	0.0	0.0022788006216568095	7.596002072189365E-5
20	1.519200414437873E-4	7.596002072189365E-5	0.0	0.0031903208703195334	1.519200414437873E-4
21	1.519200414437873E-4	7.596002072189365E-5	0.0	0.005772961574863918	1.519200414437873E-4
22	1.519200414437873E-4	7.596002072189365E-5	0.0	0.008887322424461557	1.519200414437873E-4
23	1.519200414437873E-4	7.596002072189365E-5	0.0	0.010862282963230793	1.519200414437873E-4
24	1.519200414437873E-4	7.596002072189365E-5	0.0	0.013217043605609496	1.519200414437873E-4
25	1.519200414437873E-4	7.596002072189365E-5	0.0	0.014736244020047369	1.519200414437873E-4
26	1.519200414437873E-4	7.596002072189365E-5	0.0	0.018610205076863943	1.519200414437873E-4
27	1.519200414437873E-4	7.596002072189365E-5	0.0	0.042689531645704236	1.519200414437873E-4
28	1.519200414437873E-4	7.596002072189365E-5	0.0	0.15116044123656838	1.519200414437873E-4
29	2.2788006216568096E-4	7.596002072189365E-5	0.0	0.32997033001590603	1.519200414437873E-4
30	2.2788006216568096E-4	7.596002072189365E-5	0.0	0.5542043111869361	1.519200414437873E-4
31	2.2788006216568096E-4	7.596002072189365E-5	0.0	1.226982214720748	1.519200414437873E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	30	3.5997608E-4	30.833332	5
GGTATCA	2265	0.0	28.178808	1
TGTCGCA	45	0.0038260987	20.555555	31
CGAACTA	105	2.2600943E-8	19.380953	24
GCGGTAA	905	0.0	18.80663	23
CTTATAC	1580	0.0	18.617088	37
ATACGGC	110	3.8528015E-8	18.5	29
CGAGAAC	50	0.0070354133	18.499998	3
TACAGCG	50	0.0070354133	18.499998	5
TATACAC	480	0.0	18.114582	37
TGCGGGT	840	0.0	18.059525	21
CGCGGTA	965	0.0	18.020725	22
GTATCAA	3565	0.0	17.747547	2
GTAGTAA	105	4.798567E-7	17.61905	1
TTTTACG	85	2.7236018E-5	17.411764	4
AGCCGCG	1010	0.0	17.400991	19
TTCTGCG	865	0.0	17.3237	18
TAGTACC	75	2.0675545E-4	17.266666	4
GGACGTA	140	1.869921E-9	17.178572	27
CGTGCCA	1060	0.0	17.103773	10
>>END_MODULE