FastQCFastQC Report
Fri 10 Feb 2017
ERR1631686.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631686.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133500
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5440.40749063670411984No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4930.3692883895131086No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4890.36629213483146067No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4650.34831460674157305No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4610.3453183520599251No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4130.3093632958801498No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA3930.29438202247191014No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3630.27191011235955054No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3610.2704119850187266No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3410.25543071161048686No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3290.24644194756554305No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3260.2441947565543071No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3180.2382022471910112No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3060.2292134831460674No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2870.2149812734082397No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2610.19550561797752808No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2530.1895131086142322No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2400.1797752808988764No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2380.17827715355805243No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2300.17228464419475653No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2290.17153558052434456No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2170.16254681647940075No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2150.16104868913857678No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2110.15805243445692882No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2080.15580524344569288No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2070.15505617977528088No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2060.1543071161048689No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2020.15131086142322098No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2000.149812734082397No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1980.14831460674157304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1980.14831460674157304No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1910.14307116104868914No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA1860.1393258426966292No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1830.13707865168539327No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1830.13707865168539327No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1790.13408239700374533No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1790.13408239700374533No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1750.1310861423220974No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1740.1303370786516854No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC1740.1303370786516854No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.12958801498127342No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC1730.12958801498127342No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1720.12883895131086143No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1690.12659176029962546No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC1690.12659176029962546No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1680.1258426966292135No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1650.12359550561797752No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1620.12134831460674157No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1620.12134831460674157No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA1610.12059925093632959No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1600.1198501872659176No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1600.1198501872659176No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1590.1191011235955056No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1580.11835205992509364No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA1550.11610486891385767No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1520.11385767790262173No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1510.11310861423220973No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1510.11310861423220973No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1480.11086142322097377No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC1450.10861423220973783No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT1430.10711610486891386No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT1420.10636704119850186No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1400.1048689138576779No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1370.10262172284644196No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1350.10112359550561799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACC556.192331E-726.9090937
AAGGGTT358.828223E-426.4285725
CAAGGGT451.3146734E-424.6666664
GGTATCA2950.023.830511
GGTACTT400.001922308423.1252
GGGTACT400.001922308423.1251
CCAAGGG400.001922308423.1253
ATCTAAG909.321775E-820.55555532
AGGGTTC450.00380838620.5555556
AGTCTGT450.00380838620.55555537
ACTGTGC450.00380838620.5555558
CGCGGTA1900.020.44736922
GGTAATA1750.020.08571425
TAATACG1700.019.58823627
CATCTAA951.6485865E-719.47368431
CTTATAC1254.0199666E-1019.2437
GCGGTAA1850.019.023
CCGCGGT2050.018.95122121
AGCCGCG2100.018.519
TAAAGGG500.007003152718.55
Produced by FastQC (version 0.11.5)