##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631686.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	133500
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58129588014981	34.0	31.0	34.0	31.0	34.0
2	32.74697378277153	34.0	31.0	34.0	31.0	34.0
3	32.83650936329588	34.0	31.0	34.0	31.0	34.0
4	36.25543820224719	37.0	37.0	37.0	35.0	37.0
5	36.17007490636704	37.0	35.0	37.0	35.0	37.0
6	36.22930337078652	37.0	36.0	37.0	35.0	37.0
7	36.242576779026216	37.0	37.0	37.0	35.0	37.0
8	36.21237453183521	37.0	36.0	37.0	35.0	37.0
9	38.062531835205995	39.0	38.0	39.0	37.0	39.0
10	37.98717602996255	39.0	38.0	39.0	35.0	39.0
11	38.06752808988764	39.0	38.0	39.0	37.0	39.0
12	37.98280898876404	39.0	38.0	39.0	35.0	39.0
13	38.04847191011236	39.0	38.0	39.0	35.0	39.0
14	39.400209737827716	40.0	39.0	41.0	37.0	41.0
15	39.432232209737826	40.0	39.0	41.0	37.0	41.0
16	39.34935580524345	40.0	39.0	41.0	36.0	41.0
17	39.33147565543071	40.0	39.0	41.0	36.0	41.0
18	39.35730337078652	40.0	39.0	41.0	36.0	41.0
19	39.38879400749064	40.0	39.0	41.0	37.0	41.0
20	39.39915355805243	40.0	39.0	41.0	37.0	41.0
21	39.353535580524344	40.0	39.0	41.0	36.0	41.0
22	39.3185393258427	40.0	39.0	41.0	36.0	41.0
23	39.25665168539326	40.0	39.0	41.0	36.0	41.0
24	39.274644194756554	40.0	39.0	41.0	36.0	41.0
25	39.220906367041195	40.0	39.0	41.0	36.0	41.0
26	39.153026217228465	40.0	39.0	41.0	36.0	41.0
27	39.018157303370785	40.0	39.0	41.0	35.0	41.0
28	39.02185018726592	40.0	39.0	41.0	35.0	41.0
29	39.01646441947565	40.0	39.0	41.0	35.0	41.0
30	38.978277153558054	40.0	38.0	41.0	35.0	41.0
31	38.89265168539326	40.0	38.0	41.0	35.0	41.0
32	38.861558052434454	40.0	38.0	41.0	35.0	41.0
33	38.77718352059925	40.0	38.0	41.0	35.0	41.0
34	38.7661797752809	40.0	38.0	41.0	35.0	41.0
35	38.69082397003746	40.0	38.0	41.0	35.0	41.0
36	38.63777528089888	40.0	38.0	41.0	35.0	41.0
37	38.60589513108614	40.0	38.0	41.0	35.0	41.0
38	38.55704119850187	40.0	38.0	41.0	35.0	41.0
39	38.47350561797753	40.0	38.0	41.0	34.0	41.0
40	38.16251685393259	40.0	37.0	41.0	34.0	41.0
41	38.23364794007491	40.0	37.0	41.0	34.0	41.0
42	38.211543071161046	40.0	37.0	41.0	34.0	41.0
43	37.73338576779026	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	3.0
21	6.0
22	17.0
23	34.0
24	73.0
25	97.0
26	177.0
27	266.0
28	457.0
29	663.0
30	956.0
31	1275.0
32	1693.0
33	2241.0
34	3343.0
35	5154.0
36	7823.0
37	13917.0
38	32818.0
39	62477.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.1625468164794	16.17677902621723	13.489138576779025	28.171535580524342
2	20.891385767790265	19.913857677902623	32.176779026217226	27.017977528089887
3	21.44868913857678	19.51685393258427	29.91011235955056	29.12434456928839
4	15.30187265917603	16.85243445692884	35.44794007490636	32.39775280898877
5	16.552808988764045	32.593258426966294	33.95580524344569	16.898127340823972
6	30.92059925093633	34.91460674157303	16.886142322097378	17.27865168539326
7	28.505617977528093	27.808988764044944	20.808239700374532	22.877153558052434
8	26.532584269662923	29.869662921348315	21.711610486891388	21.886142322097378
9	28.597752808988762	12.658426966292136	18.147565543071163	40.59625468164794
10	19.666666666666664	22.928838951310862	28.785767790262174	28.6187265917603
11	39.0501872659176	19.4876404494382	18.59325842696629	22.868913857677903
12	21.80299625468165	24.107116104868915	26.895880149812733	27.194007490636707
13	35.904119850187264	17.72059925093633	20.650936329588017	25.724344569288387
14	23.63820224719101	21.238202247191012	23.961797752808987	31.16179775280899
15	30.85243445692884	23.882397003745318	19.857677902621724	25.40749063670412
16	24.01722846441948	24.396254681647942	23.199250936329587	28.387265917602996
17	27.038202247191013	23.87940074906367	21.262172284644194	27.820224719101123
18	26.4936329588015	20.77977528089888	23.398501872659175	29.328089887640452
19	27.68464419475655	22.528089887640448	23.92808988764045	25.859176029962548
20	31.101872659176028	19.756554307116104	22.957303370786516	26.18426966292135
21	27.22621722846442	21.98876404494382	21.75805243445693	29.02696629213483
22	28.711610486891388	22.6187265917603	21.295131086142323	27.374531835205996
23	28.231460674157304	21.683895131086143	22.519101123595505	27.56554307116105
24	27.788014981273406	20.855430711610488	23.725093632958803	27.631460674157303
25	27.812734082397007	21.856928838951312	23.66816479400749	26.662172284644193
26	27.99176029962547	23.016479400749063	22.306367041198502	26.685393258426966
27	26.597003745318354	21.36179775280899	25.39475655430712	26.646441947565542
28	25.537827715355803	23.13632958801498	22.033707865168537	29.292134831460675
29	27.514606741573033	23.76104868913858	22.051685393258428	26.67265917602996
30	26.498127340823967	21.740823970037454	24.254681647940075	27.506367041198505
31	28.43370786516854	21.97827715355805	23.137827715355805	26.450187265917602
32	25.644194756554306	21.854681647940076	22.728089887640447	29.77303370786517
33	25.53558052434457	21.697378277153557	25.47865168539326	27.288389513108612
34	26.037453183520597	21.96479400749064	24.114606741573034	27.88314606741573
35	25.185018726591764	24.65318352059925	24.749812734082397	25.41198501872659
36	25.927340823970034	22.858426966292132	25.0187265917603	26.195505617977528
37	27.238202247191012	22.557303370786517	24.233707865168537	25.970786516853934
38	26.070411985018726	21.77752808988764	25.170786516853934	26.981273408239698
39	25.465917602996253	20.40299625468165	26.165543071161046	27.965543071161047
40	25.265168539325845	21.98876404494382	26.986516853932585	25.759550561797752
41	23.16629213483146	20.979775280898878	27.810486891385764	28.043445692883896
42	21.193258426966292	21.38501872659176	29.488389513108615	27.933333333333334
43	20.63445692883895	22.908614232209736	28.48239700374532	27.97453183520599
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	4.0
4	5.0
5	5.0
6	9.0
7	13.0
8	13.5
9	14.0
10	18.0
11	22.0
12	22.0
13	63.0
14	104.0
15	195.5
16	287.0
17	279.0
18	271.0
19	271.0
20	318.0
21	365.0
22	266.0
23	167.0
24	170.5
25	174.0
26	174.0
27	173.5
28	173.0
29	280.5
30	388.0
31	425.0
32	462.0
33	462.0
34	651.0
35	840.0
36	857.5
37	875.0
38	1350.0
39	1825.0
40	1825.0
41	2473.5
42	3122.0
43	3959.0
44	4796.0
45	8088.5
46	11381.0
47	11381.0
48	13044.0
49	14707.0
50	16819.5
51	18932.0
52	18965.5
53	18999.0
54	18999.0
55	16860.5
56	14722.0
57	13419.5
58	12117.0
59	10929.5
60	9742.0
61	9742.0
62	8596.0
63	7450.0
64	5749.5
65	4049.0
66	3479.0
67	2909.0
68	2909.0
69	2405.5
70	1902.0
71	1578.5
72	1255.0
73	927.0
74	599.0
75	599.0
76	472.5
77	346.0
78	274.0
79	202.0
80	171.0
81	140.0
82	140.0
83	100.0
84	60.0
85	51.0
86	42.0
87	35.0
88	28.0
89	28.0
90	18.5
91	9.0
92	5.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	133500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.28164794007491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.49383634015575	29.665917602996256
2	8.924588110985011	6.832958801498128
3	3.7862481900363947	4.348314606741574
4	2.046726411771612	3.134082397003745
5	1.3246976871600205	2.5355805243445695
6	1.0037960317770906	2.30561797752809
7	0.7650765076507651	2.0501872659176033
8	0.5459241576331546	1.6719101123595506
9	0.4363479826243494	1.503370786516854
>10	3.160098618557508	23.459925093632958
>50	0.31698822056118653	8.319101123595505
>100	0.19371502367628068	13.76554307116105
>500	0.001956717410871522	0.40749063670411984
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	544	0.40749063670411984	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	493	0.3692883895131086	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	489	0.36629213483146067	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	465	0.34831460674157305	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	461	0.3453183520599251	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	413	0.3093632958801498	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	393	0.29438202247191014	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	363	0.27191011235955054	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	361	0.2704119850187266	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	341	0.25543071161048686	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	329	0.24644194756554305	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	326	0.2441947565543071	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	318	0.2382022471910112	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	306	0.2292134831460674	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	287	0.2149812734082397	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	261	0.19550561797752808	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	253	0.1895131086142322	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	240	0.1797752808988764	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	238	0.17827715355805243	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	230	0.17228464419475653	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	229	0.17153558052434456	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	217	0.16254681647940075	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	215	0.16104868913857678	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	211	0.15805243445692882	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	208	0.15580524344569288	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	207	0.15505617977528088	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	206	0.1543071161048689	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	202	0.15131086142322098	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	200	0.149812734082397	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	198	0.14831460674157304	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	198	0.14831460674157304	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	191	0.14307116104868914	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	186	0.1393258426966292	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	183	0.13707865168539327	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	183	0.13707865168539327	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	179	0.13408239700374533	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	179	0.13408239700374533	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	175	0.1310861423220974	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	174	0.1303370786516854	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	174	0.1303370786516854	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	173	0.12958801498127342	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	173	0.12958801498127342	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	172	0.12883895131086143	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	169	0.12659176029962546	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	169	0.12659176029962546	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	168	0.1258426966292135	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	165	0.12359550561797752	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	162	0.12134831460674157	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	162	0.12134831460674157	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	161	0.12059925093632959	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	160	0.1198501872659176	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	160	0.1198501872659176	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	159	0.1191011235955056	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	158	0.11835205992509364	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	155	0.11610486891385767	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	152	0.11385767790262173	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	151	0.11310861423220973	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	151	0.11310861423220973	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	148	0.11086142322097377	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	145	0.10861423220973783	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	143	0.10711610486891386	No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT	142	0.10636704119850186	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	140	0.1048689138576779	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	137	0.10262172284644196	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	135	0.10112359550561799	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	7.49063670411985E-4	0.0
17	7.49063670411985E-4	0.0	0.0	0.0022471910112359553	0.0
18	7.49063670411985E-4	0.0	0.0	0.00299625468164794	0.0
19	7.49063670411985E-4	0.0	0.0	0.00299625468164794	0.0
20	7.49063670411985E-4	0.0	0.0	0.003745318352059925	0.0
21	7.49063670411985E-4	0.0	0.0	0.0044943820224719105	0.0
22	7.49063670411985E-4	0.0	0.0	0.006741573033707865	0.0
23	7.49063670411985E-4	0.0	0.0	0.00749063670411985	0.0
24	7.49063670411985E-4	0.0	0.0	0.01198501872659176	0.0
25	7.49063670411985E-4	0.0	0.0	0.012734082397003745	0.0
26	7.49063670411985E-4	0.0	0.0	0.017977528089887642	0.0
27	0.0022471910112359553	0.0	0.0	0.03146067415730337	0.0
28	0.0022471910112359553	0.0	0.0	0.10561797752808989	0.0
29	0.0022471910112359553	0.0	0.0	0.23071161048689137	0.0
30	0.0022471910112359553	0.0	0.0	0.43595505617977526	0.0
31	0.0022471910112359553	0.0	0.0	0.8816479400749063	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGACC	55	6.192331E-7	26.90909	37
AAGGGTT	35	8.828223E-4	26.428572	5
CAAGGGT	45	1.3146734E-4	24.666666	4
GGTATCA	295	0.0	23.83051	1
GGTACTT	40	0.0019223084	23.125	2
GGGTACT	40	0.0019223084	23.125	1
CCAAGGG	40	0.0019223084	23.125	3
ATCTAAG	90	9.321775E-8	20.555555	32
AGGGTTC	45	0.003808386	20.555555	6
AGTCTGT	45	0.003808386	20.555555	37
ACTGTGC	45	0.003808386	20.555555	8
CGCGGTA	190	0.0	20.447369	22
GGTAATA	175	0.0	20.085714	25
TAATACG	170	0.0	19.588236	27
CATCTAA	95	1.6485865E-7	19.473684	31
CTTATAC	125	4.0199666E-10	19.24	37
GCGGTAA	185	0.0	19.0	23
CCGCGGT	205	0.0	18.951221	21
AGCCGCG	210	0.0	18.5	19
TAAAGGG	50	0.0070031527	18.5	5
>>END_MODULE