FastQCFastQC Report
Fri 10 Feb 2017
ERR1631685.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631685.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838063
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21290.25403818090048125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20480.2443730363946386No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA17380.207382977174747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17010.20296803462269544No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14900.17779092979883374No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14650.174807860506907No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14540.17349531001845925No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13940.16633594371783506No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA13350.15929590018888795No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG13100.1563128308969612No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12420.14819888242292048No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11620.1386530606887549No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11580.13817576960204664No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10740.12815265678117277No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10510.12540823303260018No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9830.11729428455855943No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9800.11693631624352824No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC9590.11443053803830976No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9350.1115667915180601No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8590.1024982608706028No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA8580.10237893809892573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG251.23334E-437.05
GGTATCA13400.030.7873131
TAGTACA400.001930752923.1254
TTTTACG903.8253347E-922.611114
CAGTCGG2500.020.7210
CAGCGCG450.003824956220.5555559
CCGCTTA909.470568E-820.55555525
GACCGTT1001.2885721E-820.357
AAATCCG1101.7535058E-920.18181830
GTATCAA21150.019.3309692
GCAGTCG2650.018.8490569
AGTCGGT2650.018.84905611
GTCATAG500.00703332918.51
TCTAGGC701.2186971E-418.52
CTTATAC7650.018.37908637
TCTTATA15200.018.3782937
TAGGGGT1653.6379788E-1217.9393944
GTAACGT4850.017.92783526
TACGAGG1258.56744E-917.767
AAGACTT953.6040274E-617.5263165
Produced by FastQC (version 0.11.5)