##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631685.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	838063
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10916363089648	33.0	31.0	34.0	30.0	34.0
2	32.298908315961924	33.0	31.0	34.0	30.0	34.0
3	32.38418949410725	34.0	31.0	34.0	30.0	34.0
4	35.9797222881812	37.0	35.0	37.0	35.0	37.0
5	35.76982756666265	37.0	35.0	37.0	35.0	37.0
6	35.80450992347831	37.0	35.0	37.0	35.0	37.0
7	35.810448617824676	37.0	35.0	37.0	35.0	37.0
8	35.77791407089921	37.0	35.0	37.0	35.0	37.0
9	37.53498364681414	39.0	37.0	39.0	35.0	39.0
10	37.40757675735595	39.0	37.0	39.0	35.0	39.0
11	37.49974882556562	39.0	37.0	39.0	35.0	39.0
12	37.42562551979982	39.0	37.0	39.0	35.0	39.0
13	37.50070937387762	39.0	37.0	39.0	35.0	39.0
14	38.723770170023016	40.0	38.0	41.0	35.0	41.0
15	38.70786563778618	40.0	38.0	41.0	35.0	41.0
16	38.644425299768635	40.0	38.0	41.0	35.0	41.0
17	38.61519122070775	40.0	38.0	41.0	35.0	41.0
18	38.64466036562884	40.0	38.0	41.0	35.0	41.0
19	38.672053294322744	40.0	38.0	41.0	35.0	41.0
20	38.6588096598943	40.0	38.0	41.0	35.0	41.0
21	38.62807808004887	40.0	38.0	41.0	35.0	41.0
22	38.58765033177696	40.0	38.0	41.0	35.0	41.0
23	38.51887745909317	40.0	38.0	41.0	34.0	41.0
24	38.53015465424437	40.0	38.0	41.0	34.0	41.0
25	38.47015319850656	40.0	38.0	41.0	34.0	41.0
26	38.37553739993294	40.0	38.0	41.0	34.0	41.0
27	38.24977835795161	40.0	38.0	41.0	34.0	41.0
28	38.23589992637785	40.0	38.0	41.0	34.0	41.0
29	38.19850297650654	40.0	38.0	41.0	34.0	41.0
30	38.13845737134321	40.0	38.0	41.0	34.0	41.0
31	38.04394538358095	40.0	37.0	41.0	34.0	41.0
32	37.98272564234431	40.0	37.0	41.0	33.0	41.0
33	37.90536749623835	40.0	37.0	41.0	33.0	41.0
34	37.86511753889624	40.0	37.0	41.0	33.0	41.0
35	37.77252545453027	40.0	37.0	41.0	33.0	41.0
36	37.72672937476061	40.0	37.0	41.0	33.0	41.0
37	37.6686346969142	40.0	37.0	41.0	33.0	41.0
38	37.637910276435065	40.0	37.0	41.0	33.0	41.0
39	37.55310758260417	40.0	37.0	41.0	33.0	41.0
40	37.239959287070306	39.0	36.0	41.0	32.0	41.0
41	37.305065370980465	39.0	36.0	41.0	32.0	41.0
42	37.26283465562852	39.0	36.0	41.0	32.0	41.0
43	36.737487515855015	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	1.0
13	0.0
14	3.0
15	0.0
16	2.0
17	4.0
18	10.0
19	22.0
20	70.0
21	145.0
22	269.0
23	497.0
24	778.0
25	1353.0
26	2132.0
27	3275.0
28	4724.0
29	6608.0
30	9291.0
31	12573.0
32	16803.0
33	22549.0
34	32091.0
35	46753.0
36	74482.0
37	136213.0
38	259050.0
39	208363.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.29968391397782	17.485081670471075	13.564016070390888	26.65121834516021
2	20.119251178014064	20.55621116789549	33.9369474609904	25.38759019310004
3	21.01118889630016	21.014171965592084	30.03497350437855	27.939665633729206
4	15.475924840972578	16.979033795788624	34.40755647248477	33.13748489075404
5	16.27634199338236	33.651050100052146	33.80545376660227	16.267154139963225
6	32.608288398366234	34.49573600075412	16.31011033776697	16.58586526311268
7	29.20556091845124	29.100556879375418	20.80917544385088	20.884706758322466
8	26.524258916095807	31.920511942419605	20.740564850136565	20.814664291348027
9	27.914011237818638	13.838219799704795	18.780807648112376	39.4669613143642
10	18.958837223454562	24.744321130988958	30.580875184801144	25.715966460755336
11	37.30698050146588	20.5827008232078	20.02856587153949	22.081752803786827
12	21.84716423466971	24.923185965732884	27.861747863824082	25.367901935773325
13	33.86475718412578	18.52903659987376	22.24295786832255	25.36324834767792
14	23.411247125812736	21.349349631232975	24.633112307785932	30.606290935168357
15	28.484970700293417	25.373032814955437	21.35973071236888	24.782265772382267
16	24.20355032974848	25.321962668677656	23.733776577655856	26.740710423918014
17	25.43281352356565	25.012439398947333	23.298129138262873	26.256617939224142
18	25.578983918870062	22.49174584726924	24.94251625474457	26.98675397911613
19	26.910268082471127	23.498710717452028	25.014825854380874	24.576195345695968
20	28.324481572387754	22.073519532541113	24.606264684158592	24.995734210912545
21	26.221537044351077	23.670654831438686	23.572452190348457	26.535355933861776
22	26.686060594489913	23.945693820154332	23.30660105505195	26.061644530303806
23	26.41818097207489	23.366501086433836	24.20522084855196	26.010097092939315
24	26.50409336768238	22.94660425290223	24.62046409398816	25.92883828542723
25	26.39885068306321	23.228205994060115	24.832381336486637	25.540561986390042
26	26.279169943071107	23.94426194689421	24.156895126022746	25.61967298401194
27	25.67647062333023	23.263048243389818	25.454410945239204	25.606070188040757
28	25.194287302983188	24.219181612838174	23.949273503304642	26.637257580873992
29	25.810708741466932	24.611753531655733	23.890089408552818	25.687448318324517
30	25.451905167033985	23.828638181139127	25.040241604748093	25.679215047078802
31	26.459228005531806	23.584384467516166	24.361533679448918	25.594853847503114
32	24.398046447582104	23.726975179670262	24.53622221718415	27.33875615556348
33	24.652561919569294	23.133344390576845	26.22165636712276	25.99243732273111
34	24.887866425316474	23.154464521163685	25.402147571244644	26.555521482275196
35	24.64599916712705	24.408546851489685	25.888029897513672	25.057424083869588
36	24.85958692842901	23.463987790894002	26.183115111871064	25.493310168805927
37	25.432097586935587	22.99707778532163	25.730404516128264	25.84042011161452
38	24.483481552102884	22.546037708382304	26.697396257799234	26.273084481715575
39	24.137564837011062	21.658753578191618	27.75423804654304	26.449443538254286
40	23.910732248052952	22.618824599105317	28.018060694721044	25.45238245812069
41	22.55104926479274	22.057888249451413	28.9799215572099	26.411140928545944
42	21.586205333011957	22.308227424429905	29.466758465652344	26.638808776905794
43	20.804760501298826	23.054949329585007	29.195299160087014	26.944991009029156
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	20.5
2	21.0
3	58.5
4	96.0
5	96.0
6	135.0
7	174.0
8	208.0
9	242.0
10	358.5
11	475.0
12	475.0
13	969.0
14	1463.0
15	2755.5
16	4048.0
17	4024.0
18	4000.0
19	4000.0
20	4573.0
21	5146.0
22	4611.0
23	4076.0
24	4405.0
25	4734.0
26	4734.0
27	5331.0
28	5928.0
29	7874.5
30	9821.0
31	10916.5
32	12012.0
33	12012.0
34	14163.5
35	16315.0
36	17729.0
37	19143.0
38	22655.0
39	26167.0
40	26167.0
41	30823.0
42	35479.0
43	38965.0
44	42451.0
45	55451.0
46	68451.0
47	68451.0
48	74280.5
49	80110.0
50	87412.5
51	94715.0
52	94321.0
53	93927.0
54	93927.0
55	83217.0
56	72507.0
57	67263.0
58	62019.0
59	56035.5
60	50052.0
61	50052.0
62	45183.5
63	40315.0
64	33298.0
65	26281.0
66	22592.5
67	18904.0
68	18904.0
69	16255.0
70	13606.0
71	11562.5
72	9519.0
73	7725.0
74	5931.0
75	5931.0
76	4884.5
77	3838.0
78	3177.5
79	2517.0
80	2008.5
81	1500.0
82	1500.0
83	1234.5
84	969.0
85	759.0
86	549.0
87	432.0
88	315.0
89	315.0
90	242.5
91	170.0
92	108.5
93	47.0
94	27.5
95	8.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	838063.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.609689663030174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.07167928521969	43.19598987778642
2	6.307206271222397	6.257970915121218
3	1.992404896867208	2.965277658500515
4	1.057516064353346	2.098521750649542
5	0.6298782902468912	1.5624033252314162
6	0.43108336135781017	1.2831547065514113
7	0.3119792842644599	1.0834036831557892
8	0.23111432647190663	0.9172408010361223
9	0.18957475699181978	0.8464270376077686
>10	1.4388915874966235	14.458123040347845
>50	0.19701487353397418	6.825259652367978
>100	0.12763634307565266	12.315978622460548
>500	0.010394626424573653	3.581776991900578
>1k	0.0036260324736884834	2.608471937282937
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2129	0.25403818090048125	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2048	0.2443730363946386	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1738	0.207382977174747	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1701	0.20296803462269544	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1490	0.17779092979883374	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1465	0.174807860506907	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1454	0.17349531001845925	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1394	0.16633594371783506	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1335	0.15929590018888795	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1310	0.1563128308969612	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1242	0.14819888242292048	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1162	0.1386530606887549	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1158	0.13817576960204664	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1074	0.12815265678117277	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1051	0.12540823303260018	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	983	0.11729428455855943	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	980	0.11693631624352824	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	959	0.11443053803830976	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	935	0.1115667915180601	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	859	0.1024982608706028	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	858	0.10237893809892573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.3864554335413925E-4	0.0	0.0	0.0	1.1932277167706962E-4
9	2.3864554335413925E-4	0.0	0.0	1.1932277167706962E-4	1.1932277167706962E-4
10	2.3864554335413925E-4	1.1932277167706962E-4	0.0	1.1932277167706962E-4	1.1932277167706962E-4
11	2.3864554335413925E-4	1.1932277167706962E-4	0.0	1.1932277167706962E-4	1.1932277167706962E-4
12	2.3864554335413925E-4	1.1932277167706962E-4	0.0	1.1932277167706962E-4	1.1932277167706962E-4
13	2.3864554335413925E-4	1.1932277167706962E-4	0.0	1.1932277167706962E-4	1.1932277167706962E-4
14	2.3864554335413925E-4	1.1932277167706962E-4	0.0	1.1932277167706962E-4	1.1932277167706962E-4
15	4.772910867082785E-4	1.1932277167706962E-4	0.0	1.1932277167706962E-4	1.1932277167706962E-4
16	4.772910867082785E-4	1.1932277167706962E-4	0.0	4.772910867082785E-4	1.1932277167706962E-4
17	4.772910867082785E-4	1.1932277167706962E-4	0.0	8.352594017394874E-4	1.1932277167706962E-4
18	4.772910867082785E-4	1.1932277167706962E-4	0.0	0.001312550488447766	1.1932277167706962E-4
19	4.772910867082785E-4	1.1932277167706962E-4	0.0	0.0016705188034789747	1.1932277167706962E-4
20	4.772910867082785E-4	1.1932277167706962E-4	0.0	0.002505778205218462	2.3864554335413925E-4
21	5.966138583853481E-4	1.1932277167706962E-4	0.0	0.003937651465343297	2.3864554335413925E-4
22	5.966138583853481E-4	1.1932277167706962E-4	0.0	0.006443429670561759	2.3864554335413925E-4
23	5.966138583853481E-4	1.1932277167706962E-4	0.0	0.007278689072301247	2.3864554335413925E-4
24	5.966138583853481E-4	1.1932277167706962E-4	0.0	0.010023112820873849	2.3864554335413925E-4
25	5.966138583853481E-4	1.1932277167706962E-4	0.0	0.011335663309321614	2.3864554335413925E-4
26	5.966138583853481E-4	1.1932277167706962E-4	0.0	0.014557378144602494	2.3864554335413925E-4
27	5.966138583853481E-4	1.1932277167706962E-4	0.0	0.033410376069579494	2.3864554335413925E-4
28	5.966138583853481E-4	1.1932277167706962E-4	0.0	0.11729428455855943	2.3864554335413925E-4
29	5.966138583853481E-4	1.1932277167706962E-4	0.0	0.2623907749178761	2.3864554335413925E-4
30	5.966138583853481E-4	1.1932277167706962E-4	0.0	0.4442386789537302	2.3864554335413925E-4
31	5.966138583853481E-4	1.1932277167706962E-4	0.0	1.0101865850180713	2.3864554335413925E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	25	1.23334E-4	37.0	5
GGTATCA	1340	0.0	30.787313	1
TAGTACA	40	0.0019307529	23.125	4
TTTTACG	90	3.8253347E-9	22.61111	4
CAGTCGG	250	0.0	20.72	10
CAGCGCG	45	0.0038249562	20.555555	9
CCGCTTA	90	9.470568E-8	20.555555	25
GACCGTT	100	1.2885721E-8	20.35	7
AAATCCG	110	1.7535058E-9	20.181818	30
GTATCAA	2115	0.0	19.330969	2
GCAGTCG	265	0.0	18.849056	9
AGTCGGT	265	0.0	18.849056	11
GTCATAG	50	0.007033329	18.5	1
TCTAGGC	70	1.2186971E-4	18.5	2
CTTATAC	765	0.0	18.379086	37
TCTTATA	1520	0.0	18.37829	37
TAGGGGT	165	3.6379788E-12	17.939394	4
GTAACGT	485	0.0	17.927835	26
TACGAGG	125	8.56744E-9	17.76	7
AAGACTT	95	3.6040274E-6	17.526316	5
>>END_MODULE