FastQCFastQC Report
Fri 10 Feb 2017
ERR1631684.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631684.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227660
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6120.26882192743564964No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5690.24993411227268733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.2349995607484846No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5340.23456030923306687No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4790.21040147588509178No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4530.19898093648423087No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4430.19458842133005358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4400.1932706667838004No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3830.1682333304049899No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3800.1669155758587367No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3790.166476324343319No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3750.16471931828164807No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3620.15900904858121762No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3590.15769129403496443No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3510.15417728191162258No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3490.15329877888078713No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3470.1524202758499517No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3410.14978476675744531No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3390.14890626372660987No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3380.14846701221119213No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3360.14758850918035665No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3060.13441096371782482No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3060.13441096371782482No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3050.1339717122024071No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3030.13309320917157164No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2990.13133620310990074No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2980.130896951594483No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2840.1247474303786348No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2830.12430817886321707No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2560.11244838794693841No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2530.11113063340068524No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2450.1076166212773434No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2390.10498111218483705No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2380.10454186066941931No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2340.1027848546077484No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2330.10234560309233068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4000.031.4499991
TACGGCT502.7046008E-729.59999830
ATACGGC556.2247454E-726.9090929
AGTCGGT358.847473E-426.4285711
CTTAGGA509.049343E-625.8999982
GCAGTCG400.001926458823.1250029
TTAGCCC502.6928898E-422.19999929
CTTATAC1750.022.19999937
TTAGGAC603.7094156E-521.5833343
TATACAC603.7094156E-521.58333437
GTATCAA5750.021.556522
GGGGTTA705.0723283E-621.14285719
CGCGGTA2050.020.75609622
TGAAGCT450.00381653120.55555534
GTGATTC450.00381653120.55555516
GAGAGCT450.00381653120.5555556
CAGTCGG450.00381653120.55555510
CTCTATG909.3947165E-820.5555551
TCTATGG909.3947165E-820.5555552
GCGGTAA1900.020.44736923
Produced by FastQC (version 0.11.5)