FastQCFastQC Report
Fri 10 Feb 2017
ERR1631683.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631683.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361773
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7090.1959792466546702No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6920.1912801673977881No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6920.1912801673977881No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6080.16806118754025312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6080.16806118754025312No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6020.16640268897900065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.15617528118461024No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5550.15341111691585607No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5400.14926487051272483No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4970.13737896415708192No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4760.13157421919269818No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4700.12991572063144569No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4460.1232817263864357No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4440.12272889353268485No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4420.12217606067893402No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4410.12189964425205861No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4410.12189964425205861No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4330.11968831283705528No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4320.11941189641017987No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4180.11554206643392403No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4100.11333073501892071No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3990.11029015432329112No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3800.1050382422126582No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3800.1050382422126582No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3750.10365616007828114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC250.005491426729.616
GGTATCA4350.029.3448261
ATACGGC604.305548E-827.75000229
CGCGGTA2900.027.43103422
GCGGTAA2950.026.33898423
AACTCCG3150.025.2539675
TAACACC451.3207649E-424.66666635
AGTCGGT901.4188117E-1024.66666611
CCGCGGT3250.024.47692321
CGGTAAT3200.024.2812524
TAATACG3050.024.26229327
GGTAATA3300.024.10605825
CGTGCCA3300.024.10605810
GCCTTAT701.9153595E-723.78571326
AGCCGCG3350.023.74626719
CTAACTC3350.023.7462673
GCTAACT3350.023.7462672
CTCCGTG3600.023.638897
TCCGTGC3450.023.5942028
TAACTCC3400.023.3970584
Produced by FastQC (version 0.11.5)