##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631683.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361773
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.56100372332927	34.0	31.0	34.0	31.0	34.0
2	32.73071788110224	34.0	31.0	34.0	31.0	34.0
3	32.82211497264859	34.0	31.0	34.0	31.0	34.0
4	36.263134617564056	37.0	37.0	37.0	35.0	37.0
5	36.16908116415542	37.0	35.0	37.0	35.0	37.0
6	36.219397246339554	37.0	36.0	37.0	35.0	37.0
7	36.20816921107988	37.0	36.0	37.0	35.0	37.0
8	36.187001241109755	37.0	36.0	37.0	35.0	37.0
9	38.02167657619557	39.0	38.0	39.0	35.0	39.0
10	37.9497253802799	39.0	38.0	39.0	35.0	39.0
11	38.02702523405561	39.0	38.0	39.0	35.0	39.0
12	37.95538086037377	39.0	38.0	39.0	35.0	39.0
13	38.01531070588463	39.0	38.0	39.0	35.0	39.0
14	39.38487117612426	40.0	39.0	41.0	37.0	41.0
15	39.390819657630615	40.0	39.0	41.0	37.0	41.0
16	39.33303756775658	40.0	39.0	41.0	36.0	41.0
17	39.306855403802935	40.0	39.0	41.0	36.0	41.0
18	39.33331951251199	40.0	39.0	41.0	36.0	41.0
19	39.35187534724814	40.0	39.0	41.0	36.0	41.0
20	39.3489149273163	40.0	39.0	41.0	36.0	41.0
21	39.31003972104054	40.0	39.0	41.0	36.0	41.0
22	39.27758014003256	40.0	39.0	41.0	36.0	41.0
23	39.20978348301283	40.0	39.0	41.0	36.0	41.0
24	39.21479214866781	40.0	39.0	41.0	36.0	41.0
25	39.15446426350225	40.0	39.0	41.0	36.0	41.0
26	39.095723008626955	40.0	39.0	41.0	36.0	41.0
27	38.977911563328384	40.0	39.0	41.0	35.0	41.0
28	38.96914087010363	40.0	39.0	41.0	35.0	41.0
29	38.936634298303076	40.0	38.0	41.0	35.0	41.0
30	38.884908492341886	40.0	38.0	41.0	35.0	41.0
31	38.79907013513999	40.0	38.0	41.0	35.0	41.0
32	38.75087140278573	40.0	38.0	41.0	35.0	41.0
33	38.68382659844709	40.0	38.0	41.0	35.0	41.0
34	38.640821730753814	40.0	38.0	41.0	35.0	41.0
35	38.56680846829365	40.0	38.0	41.0	35.0	41.0
36	38.50815566667496	40.0	38.0	41.0	35.0	41.0
37	38.46506787405362	40.0	38.0	41.0	34.0	41.0
38	38.41732799296797	40.0	38.0	41.0	34.0	41.0
39	38.358467879029114	40.0	38.0	41.0	34.0	41.0
40	38.037462718334424	40.0	37.0	41.0	34.0	41.0
41	38.10213863389473	40.0	37.0	41.0	34.0	41.0
42	38.07717823054789	40.0	37.0	41.0	34.0	41.0
43	37.5951660295268	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	3.0
19	5.0
20	15.0
21	27.0
22	60.0
23	114.0
24	191.0
25	352.0
26	565.0
27	806.0
28	1269.0
29	1900.0
30	2690.0
31	3709.0
32	4898.0
33	6518.0
34	9532.0
35	14417.0
36	22375.0
37	39789.0
38	87471.0
39	165066.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.15026273381374	16.453134976905407	12.728976457612923	27.667625831667923
2	20.988852125504113	19.412449243033613	32.41120813327694	27.187490498185323
3	21.32276316916962	19.624737058873933	28.888280772749763	30.164218999206682
4	15.591544974334735	15.97051189558093	33.57188070972682	34.866062420357515
5	17.350106282116133	32.462068755822024	32.924237021557715	17.263587940504127
6	34.427665967333105	32.985877884750934	15.74136267770121	16.84509347021475
7	30.631915593479892	27.630862446893495	19.832325795457372	21.904896164169244
8	27.641919103968508	30.664809148278067	20.10625447449091	21.587017273262514
9	28.362813145259597	13.026400532930872	17.863688003250658	40.747098318558876
10	19.57498210203636	23.56532964040987	29.704814897739745	27.154873359814026
11	39.05377128752007	19.603729410431402	18.99975951770862	22.342739784339905
12	22.893637723102607	23.6009873594768	27.036014296257598	26.469360621162995
13	35.082772899027844	17.68982207074602	20.9642510635122	26.263153966713933
14	24.022245994034932	20.155180182047857	23.72509833514386	32.09747548877335
15	29.376708599038626	24.128113485528218	20.324899868149366	26.170278047283794
16	25.285192648428712	24.04048947820871	22.786940982328698	27.88737689103388
17	26.489262603898023	23.80664118107211	22.3889013276281	27.315194887401766
18	27.026063304890087	21.423102332125392	23.48323396162787	28.06760040135665
19	28.01314636526219	22.484817827753865	23.475494301675358	26.026541505308575
20	29.334416885726682	21.18593703786629	23.095974547575413	26.383671528831616
21	27.580554657202168	22.24709970064101	22.412396723912508	27.759948918244316
22	28.074510812028535	22.874564989648206	21.909042410572376	27.141881787750883
23	27.947082839238966	22.136533129890843	22.712308547072336	27.20407548379785
24	27.52527137182709	21.69537251259768	23.36769189519395	27.411664220381287
25	27.626163367636615	21.99003242364687	23.479364131651618	26.9044400770649
26	27.456167265108235	22.767868248874294	23.084365057646647	26.69159942837083
27	26.75462237369842	22.09589991514016	24.335702222111657	26.813775489049764
28	26.496173014569912	23.019960030184674	22.54811718950834	27.93574976573708
29	26.980178178028762	23.466648976015346	22.621920375484077	26.93125247047182
30	26.81266982334226	22.37121067630807	23.94236164666794	26.873757853681727
31	27.500117476981423	22.375909755564955	23.039862012919706	27.084110754533917
32	25.676321892457427	22.518264215405793	23.35193615886205	28.453477733274735
33	25.847700077120184	21.97704085158373	24.874714254518718	27.30054481677737
34	26.13462032821687	21.790459763442822	24.43134230581055	27.643577602529763
35	25.805684780235122	23.26873481437255	24.911477639293146	26.014102766099185
36	25.914592852424036	22.291602745367953	25.134545695781608	26.659258706426403
37	26.277527620911457	21.940277466809295	24.861446266028697	26.920748646250548
38	25.246494348666154	21.43471182205416	25.78799412891509	27.530799700364593
39	25.026743289300196	20.426068280385767	26.91162690416366	27.635561526150376
40	24.64473578735837	21.42448441425977	27.288106077567974	26.64267372081388
41	23.337009671810776	20.871927976935815	28.27380705580571	27.517255295447697
42	22.158370027614	21.25752889242702	29.1105195799576	27.47358150000138
43	21.622951408756318	21.807597581909096	28.566808468293654	28.002642541040927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	3.5
2	3.0
3	14.0
4	25.0
5	25.0
6	37.5
7	50.0
8	58.0
9	66.0
10	97.0
11	128.0
12	128.0
13	253.5
14	379.0
15	712.5
16	1046.0
17	1005.0
18	964.0
19	964.0
20	1152.5
21	1341.0
22	1099.0
23	857.0
24	935.0
25	1013.0
26	1013.0
27	1254.0
28	1495.0
29	1758.5
30	2022.0
31	2518.0
32	3014.0
33	3014.0
34	3869.5
35	4725.0
36	5418.0
37	6111.0
38	7453.0
39	8795.0
40	8795.0
41	10372.5
42	11950.0
43	14169.5
44	16389.0
45	22037.0
46	27685.0
47	27685.0
48	30569.5
49	33454.0
50	36325.5
51	39197.0
52	39003.5
53	38810.0
54	38810.0
55	35893.0
56	32976.0
57	31598.0
58	30220.0
59	28068.0
60	25916.0
61	25916.0
62	23864.5
63	21813.0
64	18636.5
65	15460.0
66	13551.5
67	11643.0
68	11643.0
69	10127.0
70	8611.0
71	7217.0
72	5823.0
73	4734.0
74	3645.0
75	3645.0
76	3018.5
77	2392.0
78	1985.0
79	1578.0
80	1294.0
81	1010.0
82	1010.0
83	788.0
84	566.0
85	456.5
86	347.0
87	250.5
88	154.0
89	154.0
90	111.5
91	69.0
92	46.5
93	24.0
94	13.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	361773.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.12030917834163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.22630571240883	55.42765697696066
2	5.825812357505297	7.238025297264647
3	1.7200325252571476	3.2054685679733317
4	0.7923802050810851	1.9689161330573899
5	0.4670100739995236	1.4505405093125305
6	0.33312212331541907	1.2416189576699688
7	0.21946341818340578	0.9543194773622193
8	0.1582183224334184	0.7862856885793997
9	0.14417337915232356	0.806048539940575
>10	0.9286155625434213	11.357034905639713
>50	0.1040431221932359	4.592471624337897
>100	0.07680436488082511	9.426272694988086
>500	0.004018833046089685	1.5453406269136196
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	709	0.1959792466546702	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	692	0.1912801673977881	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	692	0.1912801673977881	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	608	0.16806118754025312	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.16806118754025312	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	602	0.16640268897900065	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	565	0.15617528118461024	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	555	0.15341111691585607	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	540	0.14926487051272483	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	497	0.13737896415708192	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	476	0.13157421919269818	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	470	0.12991572063144569	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	446	0.1232817263864357	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	444	0.12272889353268485	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	442	0.12217606067893402	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	441	0.12189964425205861	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	441	0.12189964425205861	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	433	0.11968831283705528	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	432	0.11941189641017987	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	418	0.11554206643392403	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	410	0.11333073501892071	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	399	0.11029015432329112	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	380	0.1050382422126582	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	380	0.1050382422126582	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	375	0.10365616007828114	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	2.7641642687541634E-4	0.0
15	0.0	0.0	0.0	2.7641642687541634E-4	0.0
16	0.0	0.0	0.0	2.7641642687541634E-4	0.0
17	0.0	0.0	0.0	8.29249280626249E-4	0.0
18	0.0	0.0	0.0	8.29249280626249E-4	0.0
19	0.0	0.0	0.0	0.001658498561252498	0.0
20	0.0	0.0	0.0	0.002487747841878747	0.0
21	0.0	0.0	0.0	0.0038698299762558288	0.0
22	0.0	0.0	0.0	0.0060811613912591595	0.0
23	0.0	0.0	0.0	0.006910410671885409	0.0
24	0.0	0.0	0.0	0.010227407794390405	0.0
25	0.0	0.0	0.0	0.011609489928767487	0.0
26	0.0	0.0	0.0	0.015755736331898734	0.0
27	0.0	0.0	0.0	0.03648696834755496	0.0
28	0.0	0.0	0.0	0.13074496991207193	0.0
29	0.0	0.0	0.0	0.2769692597291672	0.0
30	0.0	0.0	0.0	0.4768183363600932	0.0
31	0.0	0.0	0.0	1.0592277477865955	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTC	25	0.0054914267	29.6	16
GGTATCA	435	0.0	29.344826	1
ATACGGC	60	4.305548E-8	27.750002	29
CGCGGTA	290	0.0	27.431034	22
GCGGTAA	295	0.0	26.338984	23
AACTCCG	315	0.0	25.253967	5
TAACACC	45	1.3207649E-4	24.666666	35
AGTCGGT	90	1.4188117E-10	24.666666	11
CCGCGGT	325	0.0	24.476923	21
CGGTAAT	320	0.0	24.28125	24
TAATACG	305	0.0	24.262293	27
GGTAATA	330	0.0	24.106058	25
CGTGCCA	330	0.0	24.106058	10
GCCTTAT	70	1.9153595E-7	23.785713	26
AGCCGCG	335	0.0	23.746267	19
CTAACTC	335	0.0	23.746267	3
GCTAACT	335	0.0	23.746267	2
CTCCGTG	360	0.0	23.63889	7
TCCGTGC	345	0.0	23.594202	8
TAACTCC	340	0.0	23.397058	4
>>END_MODULE