FastQCFastQC Report
Fri 10 Feb 2017
ERR1631682.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631682.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047543
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22480.21459739600188252No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA21260.20295109604092626No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA20480.19550510098392143No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17560.16763035025769826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17490.1669621199320696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17020.16247543060284877No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA14470.1381327544549484No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA13410.12801383809542902No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13260.12658191596908194No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA12420.11856315206153828No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC11750.11216723323052133No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11610.11083077257926403No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC11330.10815785127674951No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11130.1062486217749534No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11130.1062486217749534No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC11120.10615316029986359No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10960.1046257766984267No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10820.10328931604716943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12900.031.9806211
CTAGTTC951.0913936E-1125.315793
TATAACT551.9024505E-523.5454544
ATACGGC1155.456968E-1222.5217429
GTACTAT502.7018803E-422.21
TTAGAGC450.003825584720.5555573
CATAATA450.003825584720.5555572
GTATACT450.003825584720.5555574
GCGGTAA7300.020.27397223
CGCGGTA7450.019.86577222
TAATACT759.264486E-619.7333344
CCAATAC759.264486E-619.7333343
TTCTGCG5550.019.33333418
TAGTTCC1254.129106E-1019.2400024
CGTTAGA1254.129106E-1019.2400021
TACGGCT1457.2759576E-1219.1379330
CTTATAC9650.018.97927537
TCTTATA19900.018.96482537
GTATCAA22000.018.7522722
CCTAGTT1501.2732926E-1118.52
Produced by FastQC (version 0.11.5)