##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631682.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1047543
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53453939360962	34.0	31.0	34.0	31.0	34.0
2	32.701338274419285	34.0	31.0	34.0	31.0	34.0
3	32.79074462814414	34.0	31.0	34.0	31.0	34.0
4	36.25494609767809	37.0	37.0	37.0	35.0	37.0
5	36.156106240984855	37.0	35.0	37.0	35.0	37.0
6	36.2056326088762	37.0	36.0	37.0	35.0	37.0
7	36.19769116876348	37.0	36.0	37.0	35.0	37.0
8	36.17571593719781	37.0	36.0	37.0	35.0	37.0
9	38.000439122785416	39.0	38.0	39.0	35.0	39.0
10	37.92240891304701	39.0	38.0	39.0	35.0	39.0
11	38.00031311363829	39.0	38.0	39.0	35.0	39.0
12	37.93467571259605	39.0	38.0	39.0	35.0	39.0
13	37.98543162428655	39.0	38.0	39.0	35.0	39.0
14	39.35638441572327	40.0	39.0	41.0	37.0	41.0
15	39.36170925680378	40.0	39.0	41.0	36.0	41.0
16	39.310566726139164	40.0	39.0	41.0	36.0	41.0
17	39.29066205396819	40.0	39.0	41.0	36.0	41.0
18	39.30877777809598	40.0	39.0	41.0	36.0	41.0
19	39.33681481332986	40.0	39.0	41.0	36.0	41.0
20	39.330411257580835	40.0	39.0	41.0	36.0	41.0
21	39.294203674694025	40.0	39.0	41.0	36.0	41.0
22	39.26875269082033	40.0	39.0	41.0	36.0	41.0
23	39.20074975442535	40.0	39.0	41.0	36.0	41.0
24	39.22281758362187	40.0	39.0	41.0	36.0	41.0
25	39.16334985771467	40.0	39.0	41.0	36.0	41.0
26	39.096404634463696	40.0	39.0	41.0	36.0	41.0
27	38.98013924010757	40.0	39.0	41.0	35.0	41.0
28	38.97610694739977	40.0	39.0	41.0	35.0	41.0
29	38.952980450444514	40.0	38.0	41.0	35.0	41.0
30	38.90732504536807	40.0	38.0	41.0	35.0	41.0
31	38.824570447227465	40.0	38.0	41.0	35.0	41.0
32	38.76707591001038	40.0	38.0	41.0	35.0	41.0
33	38.70916611537665	40.0	38.0	41.0	35.0	41.0
34	38.674592832943375	40.0	38.0	41.0	35.0	41.0
35	38.60030853148749	40.0	38.0	41.0	35.0	41.0
36	38.542144809330026	40.0	38.0	41.0	35.0	41.0
37	38.50333112817326	40.0	38.0	41.0	35.0	41.0
38	38.45191462307514	40.0	38.0	41.0	34.0	41.0
39	38.38450832089948	40.0	38.0	41.0	34.0	41.0
40	38.084484360069226	40.0	37.0	41.0	34.0	41.0
41	38.149243515540654	40.0	37.0	41.0	34.0	41.0
42	38.12263172012987	40.0	37.0	41.0	34.0	41.0
43	37.64548567457374	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	4.0
18	6.0
19	30.0
20	43.0
21	90.0
22	192.0
23	330.0
24	553.0
25	996.0
26	1569.0
27	2426.0
28	3611.0
29	5251.0
30	7497.0
31	10267.0
32	14098.0
33	19033.0
34	27495.0
35	41259.0
36	64214.0
37	116796.0
38	260636.0
39	471144.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.01665898201792	17.007034556099367	12.911928197696895	27.064378264185812
2	19.70363030443619	20.47276340923475	33.973116139385205	25.850490146943848
3	20.277258308250833	20.656431287307537	30.42214018899463	28.644170215447
4	14.87728904684581	16.106069154201784	34.579582890630746	34.43705890832166
5	15.73119194152412	34.578055507029305	33.72300707464992	15.967745476796658
6	33.15052460853636	35.13001375599856	15.856437396841944	15.863024238623142
7	29.635919480154993	29.381801033465933	19.990492037081058	20.991787449298023
8	26.834220647744296	32.47637567145215	20.33195773347729	20.357445947326266
9	27.6057402894201	13.643640404260255	17.707053552932912	41.04356575338674
10	18.37490203266119	24.924322915622557	30.278279746034293	26.422495305681963
11	38.07805502972193	20.411095296326735	20.124901794007503	21.38594787994383
12	22.35392723735446	24.415131407493533	27.521161422490533	25.709779932661476
13	33.7568958983068	18.176055780049126	22.474686003343063	25.592362318301014
14	23.349876806966396	19.99373772723411	24.2899814136508	32.366404052148695
15	28.25688301100766	25.171186290204794	21.16877302411452	25.40315767467302
16	24.857977190435143	24.954202357325666	22.84335822013989	27.344462232099303
17	26.054109473310405	24.571879149590995	22.732718370510803	26.641293006587794
18	25.733549839958837	22.44681125261684	24.409594641938327	27.410044265486
19	27.852985509902695	23.10769104466356	24.198720243464948	24.840603201968797
20	28.668512891594904	21.789749919573705	23.923409349305945	25.61832783952544
21	26.530844079908896	23.321620210339812	22.8329529193551	27.31458279039619
22	26.901711910632788	23.767234376059026	22.358700311108947	26.97235340219924
23	26.662294531107555	23.389970626504113	23.386343090450705	26.56139175193763
24	27.15878966304963	22.58904885050065	23.678646127175686	26.573515359274037
25	27.20480209404292	22.947888535363226	24.12368752404436	25.7236218465495
26	27.128146529545806	23.27828070064904	23.555596285784926	26.037976484020227
27	26.491704875122075	22.699975084555003	24.53913586363519	26.269184176687737
28	25.626346603432985	24.02946704813072	23.291358922736347	27.05282742569995
29	26.139642954990872	24.59918113146668	23.092035362748835	26.169140550793617
30	25.693837866321477	24.145739124790104	24.23012706876949	25.930295940118924
31	26.822574347783334	23.468535420503027	23.685232968956882	26.023657262756757
32	24.33360730776684	23.68676035255832	24.25093767033907	27.72869466933577
33	25.07066535693523	23.041058935050877	25.3743283091959	26.513947398817994
34	25.21614864497209	23.129647183934214	24.930909757403754	26.723294413689942
35	24.968617040064224	24.276139499762778	25.058350826648645	25.696892633524353
36	25.190564969648022	23.46099396397093	25.693265097470942	25.655175968910104
37	26.28102139959887	22.727372527905775	24.708484520444507	26.283121552050847
38	24.76232479239516	22.599454151285435	26.149475486925116	26.48874556939429
39	24.404439722283477	21.55405553757698	26.951542800629664	27.089961939509884
40	24.27890788254038	22.220949402554357	27.786639784715284	25.71350293018998
41	22.967076291856277	21.753188174614312	28.602930858208207	26.676804675321204
42	22.367100920916847	21.667941077359114	29.15040241784824	26.814555583875794
43	21.16638648723728	22.27354867532884	29.35612189666677	27.203942940767114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	10.5
2	15.0
3	51.5
4	88.0
5	88.0
6	123.0
7	158.0
8	179.5
9	201.0
10	301.5
11	402.0
12	402.0
13	768.0
14	1134.0
15	2560.0
16	3986.0
17	3933.5
18	3881.0
19	3881.0
20	4232.0
21	4583.0
22	4370.5
23	4158.0
24	4637.0
25	5116.0
26	5116.0
27	5701.0
28	6286.0
29	8651.0
30	11016.0
31	12433.5
32	13851.0
33	13851.0
34	16570.0
35	19289.0
36	20886.5
37	22484.0
38	27958.5
39	33433.0
40	33433.0
41	40078.5
42	46724.0
43	49117.5
44	51511.0
45	65514.5
46	79518.0
47	79518.0
48	87482.5
49	95447.0
50	104350.0
51	113253.0
52	116599.0
53	119945.0
54	119945.0
55	105921.0
56	91897.0
57	85158.0
58	78419.0
59	71400.5
60	64382.0
61	64382.0
62	58904.0
63	53426.0
64	45026.0
65	36626.0
66	32386.5
67	28147.0
68	28147.0
69	24362.0
70	20577.0
71	17617.0
72	14657.0
73	12044.5
74	9432.0
75	9432.0
76	7609.5
77	5787.0
78	4698.0
79	3609.0
80	2769.0
81	1929.0
82	1929.0
83	1504.0
84	1079.0
85	844.0
86	609.0
87	469.0
88	329.0
89	329.0
90	229.5
91	130.0
92	72.5
93	15.0
94	10.5
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1047543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.93089362104272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.04570401220042	39.52156077532106
2	7.036378954844167	6.463743465045825
3	2.1032919013018687	2.8981822971809046
4	1.1021026805351708	2.024822439165079
5	0.6445926827279012	1.4803358969638887
6	0.4380436962645224	1.2071843048696478
7	0.32323831573976414	1.039263728614169
8	0.24599667811488063	0.9039077802899577
9	0.1923107055089071	0.7949702301225557
>10	1.4687204262226428	13.762481245534977
>50	0.21076734218601884	6.857958455198911
>100	0.16944565165326603	15.473950501749718
>500	0.015233414485314097	4.88868490033177
>1k	0.004173538215154547	2.6829539796115456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2248	0.21459739600188252	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	2126	0.20295109604092626	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	2048	0.19550510098392143	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1756	0.16763035025769826	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1749	0.1669621199320696	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1702	0.16247543060284877	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1447	0.1381327544549484	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1341	0.12801383809542902	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1326	0.12658191596908194	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1242	0.11856315206153828	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1175	0.11216723323052133	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1161	0.11083077257926403	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	1133	0.10815785127674951	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1113	0.1062486217749534	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1113	0.1062486217749534	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1112	0.10615316029986359	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1096	0.1046257766984267	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1082	0.10328931604716943	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.546147508980539E-5
2	0.0	0.0	0.0	0.0	9.546147508980539E-5
3	0.0	0.0	0.0	0.0	9.546147508980539E-5
4	0.0	0.0	0.0	0.0	9.546147508980539E-5
5	0.0	0.0	0.0	0.0	9.546147508980539E-5
6	0.0	0.0	0.0	0.0	9.546147508980539E-5
7	0.0	0.0	0.0	0.0	9.546147508980539E-5
8	0.0	0.0	0.0	0.0	9.546147508980539E-5
9	0.0	0.0	0.0	0.0	9.546147508980539E-5
10	0.0	0.0	0.0	0.0	9.546147508980539E-5
11	0.0	0.0	0.0	0.0	9.546147508980539E-5
12	0.0	0.0	0.0	0.0	9.546147508980539E-5
13	9.546147508980539E-5	0.0	0.0	0.0	9.546147508980539E-5
14	9.546147508980539E-5	0.0	0.0	2.8638442526941613E-4	9.546147508980539E-5
15	9.546147508980539E-5	0.0	0.0	4.773073754490269E-4	9.546147508980539E-5
16	9.546147508980539E-5	0.0	0.0	7.636918007184431E-4	9.546147508980539E-5
17	9.546147508980539E-5	0.0	0.0	0.00124099917616747	9.546147508980539E-5
18	9.546147508980539E-5	0.0	0.0	0.0014319221263470808	9.546147508980539E-5
19	9.546147508980539E-5	0.0	0.0	0.0017183065516164968	2.8638442526941613E-4
20	9.546147508980539E-5	0.0	0.0	0.0021956139270655236	2.8638442526941613E-4
21	9.546147508980539E-5	0.0	0.0	0.004486689329220853	2.8638442526941613E-4
22	9.546147508980539E-5	0.0	0.0	0.006968687681555793	2.8638442526941613E-4
23	9.546147508980539E-5	0.0	0.0	0.008209686857723263	2.8638442526941613E-4
24	9.546147508980539E-5	0.0	0.0	0.009641608984070344	3.8184590035922155E-4
25	9.546147508980539E-5	0.0	0.0	0.010500762259878593	3.8184590035922155E-4
26	9.546147508980539E-5	0.0	0.0	0.011646299960956256	3.8184590035922155E-4
27	9.546147508980539E-5	0.0	0.0	0.021956139270655237	3.8184590035922155E-4
28	9.546147508980539E-5	0.0	0.0	0.09660701279088305	3.8184590035922155E-4
29	9.546147508980539E-5	0.0	0.0	0.2233798517101446	3.8184590035922155E-4
30	1.9092295017961077E-4	0.0	0.0	0.3776455954552701	3.8184590035922155E-4
31	1.9092295017961077E-4	0.0	0.0	0.8828277216305201	3.8184590035922155E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1290	0.0	31.980621	1
CTAGTTC	95	1.0913936E-11	25.31579	3
TATAACT	55	1.9024505E-5	23.545454	4
ATACGGC	115	5.456968E-12	22.52174	29
GTACTAT	50	2.7018803E-4	22.2	1
TTAGAGC	45	0.0038255847	20.555557	3
CATAATA	45	0.0038255847	20.555557	2
GTATACT	45	0.0038255847	20.555557	4
GCGGTAA	730	0.0	20.273972	23
CGCGGTA	745	0.0	19.865772	22
TAATACT	75	9.264486E-6	19.733334	4
CCAATAC	75	9.264486E-6	19.733334	3
TTCTGCG	555	0.0	19.333334	18
TAGTTCC	125	4.129106E-10	19.240002	4
CGTTAGA	125	4.129106E-10	19.240002	1
TACGGCT	145	7.2759576E-12	19.13793	30
CTTATAC	965	0.0	18.979275	37
TCTTATA	1990	0.0	18.964825	37
GTATCAA	2200	0.0	18.752272	2
CCTAGTT	150	1.2732926E-11	18.5	2
>>END_MODULE