FastQCFastQC Report
Fri 10 Feb 2017
ERR1631681.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631681.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12688
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC270.21279949558638084No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA250.19703656998738966No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA250.19703656998738966No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT240.18915510718789408No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA230.1812736443883985No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT230.1812736443883985No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC220.1733921815889029No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA210.16551071878940732No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA200.15762925598991173No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC200.15762925598991173No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT190.14974779319041615No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA190.14974779319041615No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT170.13398486759142497No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC170.13398486759142497No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA170.13398486759142497No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA170.13398486759142497No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA170.13398486759142497No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA160.12610340479192939No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA160.12610340479192939No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA160.12610340479192939No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA160.12610340479192939No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA160.12610340479192939No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC160.12610340479192939No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT160.12610340479192939No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA150.11822194199243378No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG150.11822194199243378No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC140.11034047919293823No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG140.11034047919293823No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG140.11034047919293823No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC130.10245901639344263No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT130.10245901639344263No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG130.10245901639344263No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA130.10245901639344263No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTGC200.001773988537.08
CTCCGTG200.001773988537.07
GTCCTTC303.403934E-430.83333430
TCTGTCT303.403934E-430.83333436
CAGATCA250.005295858329.621
AGATCAC250.005295858329.622
CCCTGTC554.76741E-420.18181836
TGTCTCT1050.00240309712.33333437
CTGTCTC1654.2829066E-511.21212137
CCTGTCT1200.006524368710.79166737
Produced by FastQC (version 0.11.5)