##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631681.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12688
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47604035308953	34.0	31.0	34.0	31.0	34.0
2	32.641078184110974	34.0	31.0	34.0	31.0	34.0
3	32.738414249684745	34.0	31.0	34.0	31.0	34.0
4	36.17488965952081	37.0	35.0	37.0	35.0	37.0
5	36.11223203026482	37.0	35.0	37.0	35.0	37.0
6	36.1609394703657	37.0	36.0	37.0	35.0	37.0
7	36.126970365699876	37.0	36.0	37.0	35.0	37.0
8	36.110813366960905	37.0	35.0	37.0	35.0	37.0
9	37.96295712484237	39.0	38.0	39.0	35.0	39.0
10	37.853956494325345	39.0	38.0	39.0	35.0	39.0
11	37.944120428751575	39.0	38.0	39.0	35.0	39.0
12	37.86703972257251	39.0	38.0	39.0	35.0	39.0
13	37.91440731399748	39.0	38.0	39.0	35.0	39.0
14	39.223991172761664	40.0	39.0	41.0	36.0	41.0
15	39.29437263556116	40.0	39.0	41.0	36.0	41.0
16	39.22241488020177	40.0	39.0	41.0	36.0	41.0
17	39.18970680958386	40.0	39.0	41.0	36.0	41.0
18	39.22052332912989	40.0	39.0	41.0	36.0	41.0
19	39.23644388398487	40.0	39.0	41.0	36.0	41.0
20	39.19601197982345	40.0	39.0	41.0	36.0	41.0
21	39.136034047919296	40.0	39.0	41.0	36.0	41.0
22	39.118931273644385	40.0	39.0	41.0	36.0	41.0
23	39.02214691046658	40.0	39.0	41.0	36.0	41.0
24	39.04500315258512	40.0	39.0	41.0	35.0	41.0
25	39.01505359394704	40.0	39.0	41.0	35.0	41.0
26	38.92733291298865	40.0	38.0	41.0	35.0	41.0
27	38.85576923076923	40.0	38.0	41.0	35.0	41.0
28	38.809583858764185	40.0	38.0	41.0	35.0	41.0
29	38.813209331651954	40.0	38.0	41.0	35.0	41.0
30	38.695066204287514	40.0	38.0	41.0	35.0	41.0
31	38.63768915510719	40.0	38.0	41.0	35.0	41.0
32	38.63666456494325	40.0	38.0	41.0	35.0	41.0
33	38.56281525851198	40.0	38.0	41.0	35.0	41.0
34	38.51781210592686	40.0	38.0	41.0	35.0	41.0
35	38.42520491803279	40.0	38.0	41.0	34.0	41.0
36	38.37712799495586	40.0	38.0	41.0	34.0	41.0
37	38.32219419924338	40.0	38.0	41.0	34.0	41.0
38	38.33110025220681	40.0	38.0	41.0	34.0	41.0
39	38.20200189155107	40.0	37.0	41.0	34.0	41.0
40	37.91519546027743	40.0	37.0	41.0	33.0	41.0
41	37.98400063051702	40.0	37.0	41.0	33.0	41.0
42	37.98203026481715	40.0	37.0	41.0	33.0	41.0
43	37.480375157629254	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	1.0
23	5.0
24	11.0
25	16.0
26	20.0
27	40.0
28	51.0
29	84.0
30	110.0
31	133.0
32	201.0
33	224.0
34	350.0
35	601.0
36	831.0
37	1405.0
38	3060.0
39	5542.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.01702395964691	16.188524590163937	12.917717528373265	27.876733921815887
2	21.327238335435055	18.25346784363178	32.5031525851198	27.91614123581337
3	21.193253467843633	19.388398486759144	27.994955863808325	31.423392181588905
4	15.99936948297604	15.439785624211854	33.062736443883985	35.49810844892812
5	17.599306431273646	33.12578814627995	31.56525851197982	17.709646910466585
6	34.418348045397224	32.76324085750315	15.818095838587642	17.000315258511982
7	31.13965952080706	27.466897856242117	19.364754098360656	22.028688524590166
8	27.93190416141236	30.493379571248425	19.9952711223203	21.579445145018916
9	28.59394703656999	13.311790668348046	17.21311475409836	40.88114754098361
10	20.223833543505677	23.415825977301388	28.688524590163933	27.671815889029006
11	39.55706179066835	19.136191677175283	18.2140605296343	23.09268600252207
12	23.967528373266077	23.3921815889029	25.0	27.640290037831022
13	34.80453972257251	16.874211853720052	20.8937578814628	27.42749054224464
14	24.298549810844893	19.83764186633039	22.903530895334175	32.96027742749054
15	30.359394703657	23.494640605296343	18.781525851197983	27.364438839848678
16	25.346784363177804	24.006935687263557	21.76860025220681	28.877679697351827
17	26.875788146279948	23.447351828499368	21.871059268600252	27.80580075662043
18	27.395964691046657	20.452395964691046	23.494640605296343	28.65699873896595
19	28.90920554854981	22.028688524590166	22.343947036569986	26.718158890290038
20	29.815573770491806	20.097730138713747	23.02175283732661	27.064943253467845
21	28.262925598991174	21.997162673392182	21.603089533417403	28.136822194199247
22	29.279634300126105	22.864123581336695	20.665195460277427	27.191046658259772
23	28.570302648171502	22.16267339218159	21.610970996216896	27.65605296343001
24	28.02648171500631	21.610970996216896	21.7843631778058	28.578184110970994
25	28.53877679697352	21.185372005044137	22.74590163934426	27.529949558638084
26	28.720050441361916	22.430643127364437	22.39123581336696	26.45807061790668
27	27.356557377049178	21.46122320302648	23.076923076923077	28.105296343001264
28	26.686633039092055	22.312421185372006	22.351828499369482	28.649117276166457
29	28.27080706179067	22.89564943253468	21.64249684741488	27.191046658259772
30	26.324085750315255	21.41393442622951	24.117276166456495	28.144703656998736
31	27.845208070617904	21.713430012610342	22.840479192938208	27.600882723833543
32	26.529003783102144	21.28783102143758	22.4936948297604	29.689470365699872
33	26.599936948297604	21.871059268600252	23.841424968474147	27.687578814627994
34	26.434426229508194	21.555800756620428	23.447351828499368	28.562421185372006
35	26.347730138713743	22.540983606557376	24.542875157629258	26.56841109709962
36	26.49747793190416	22.21784363177806	23.691677175283733	27.59300126103405
37	26.00094577553594	22.20996216897856	24.32219419924338	27.466897856242117
38	25.496532156368225	20.27900378310214	25.866960907944513	28.357503152585117
39	24.73203026481715	20.720365699873895	26.379255989911726	28.168348045397224
40	24.511349306431274	21.145964691046657	28.097414880201764	26.2452711223203
41	23.313366960907945	20.468158890290038	28.207755359394703	28.010718789407314
42	22.005044136191675	20.885876418663305	29.263871374527113	27.845208070617904
43	21.082912988650694	20.64155107187894	29.27175283732661	29.00378310214376
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	1.0
11	2.0
12	2.0
13	8.0
14	14.0
15	16.5
16	19.0
17	20.5
18	22.0
19	22.0
20	26.0
21	30.0
22	23.0
23	16.0
24	20.0
25	24.0
26	24.0
27	24.5
28	25.0
29	32.0
30	39.0
31	51.0
32	63.0
33	63.0
34	100.0
35	137.0
36	159.5
37	182.0
38	211.5
39	241.0
40	241.0
41	277.0
42	313.0
43	420.5
44	528.0
45	735.5
46	943.0
47	943.0
48	1044.5
49	1146.0
50	1277.5
51	1409.0
52	1430.5
53	1452.0
54	1452.0
55	1395.0
56	1338.0
57	1224.0
58	1110.0
59	1022.5
60	935.0
61	935.0
62	886.0
63	837.0
64	659.5
65	482.0
66	422.5
67	363.0
68	363.0
69	316.5
70	270.0
71	254.0
72	238.0
73	228.0
74	218.0
75	218.0
76	200.5
77	183.0
78	120.5
79	58.0
80	40.0
81	22.0
82	22.0
83	17.5
84	13.0
85	12.5
86	12.0
87	7.5
88	3.0
89	3.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	12688.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.41771752837326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.68941373184241	66.13335435056746
2	6.479255930609259	9.773013871374527
3	2.24683875013063	5.083543505674653
4	1.2331487093740203	3.720050441361917
5	0.6792768314348416	2.5614754098360657
6	0.32396279653046295	1.4659520807061792
7	0.3030619709478525	1.599936948297604
8	0.21945866861741037	1.3240857503152585
9	0.17765701745218937	1.2058638083228248
>10	0.6479255930609259	7.132723833543506
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	27	0.21279949558638084	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	25	0.19703656998738966	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	25	0.19703656998738966	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	24	0.18915510718789408	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	23	0.1812736443883985	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	23	0.1812736443883985	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	22	0.1733921815889029	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	21	0.16551071878940732	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	20	0.15762925598991173	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	20	0.15762925598991173	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	19	0.14974779319041615	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	19	0.14974779319041615	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	17	0.13398486759142497	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	17	0.13398486759142497	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	17	0.13398486759142497	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	17	0.13398486759142497	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	17	0.13398486759142497	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	16	0.12610340479192939	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	16	0.12610340479192939	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	16	0.12610340479192939	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	16	0.12610340479192939	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	16	0.12610340479192939	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	16	0.12610340479192939	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	16	0.12610340479192939	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	15	0.11822194199243378	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	15	0.11822194199243378	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	14	0.11034047919293823	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	14	0.11034047919293823	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	14	0.11034047919293823	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	13	0.10245901639344263	No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT	13	0.10245901639344263	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	13	0.10245901639344263	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	13	0.10245901639344263	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.007881462799495587	0.0
3	0.0	0.0	0.0	0.007881462799495587	0.0
4	0.0	0.0	0.0	0.007881462799495587	0.0
5	0.0	0.0	0.0	0.007881462799495587	0.0
6	0.0	0.0	0.0	0.007881462799495587	0.0
7	0.0	0.0	0.0	0.007881462799495587	0.0
8	0.0	0.0	0.0	0.007881462799495587	0.0
9	0.0	0.0	0.0	0.007881462799495587	0.0
10	0.0	0.0	0.0	0.007881462799495587	0.0
11	0.0	0.0	0.0	0.007881462799495587	0.0
12	0.0	0.0	0.0	0.007881462799495587	0.0
13	0.0	0.0	0.0	0.007881462799495587	0.0
14	0.0	0.0	0.0	0.007881462799495587	0.0
15	0.0	0.0	0.0	0.007881462799495587	0.0
16	0.0	0.0	0.0	0.007881462799495587	0.0
17	0.0	0.0	0.0	0.007881462799495587	0.0
18	0.0	0.0	0.0	0.007881462799495587	0.0
19	0.0	0.0	0.0	0.007881462799495587	0.0
20	0.0	0.0	0.0	0.007881462799495587	0.0
21	0.0	0.0	0.0	0.007881462799495587	0.0
22	0.0	0.0	0.0	0.007881462799495587	0.0
23	0.0	0.0	0.0	0.007881462799495587	0.0
24	0.0	0.0	0.0	0.007881462799495587	0.0
25	0.0	0.0	0.0	0.007881462799495587	0.0
26	0.0	0.0	0.0	0.015762925598991173	0.0
27	0.0	0.0	0.0	0.02364438839848676	0.0
28	0.0	0.0	0.0	0.13398486759142497	0.0
29	0.0	0.0	0.0	0.2837326607818411	0.0
30	0.0	0.0	0.0	0.4650063051702396	0.0
31	0.0	0.0	0.0	1.0403530895334174	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGTGC	20	0.0017739885	37.0	8
CTCCGTG	20	0.0017739885	37.0	7
GTCCTTC	30	3.403934E-4	30.833334	30
TCTGTCT	30	3.403934E-4	30.833334	36
CAGATCA	25	0.0052958583	29.6	21
AGATCAC	25	0.0052958583	29.6	22
CCCTGTC	55	4.76741E-4	20.181818	36
TGTCTCT	105	0.002403097	12.333334	37
CTGTCTC	165	4.2829066E-5	11.212121	37
CCTGTCT	120	0.0065243687	10.791667	37
>>END_MODULE