FastQCFastQC Report
Fri 10 Feb 2017
ERR1631680.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631680.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686816
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13150.19146321576666822No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12390.18039766109118016No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11670.1699145040301915No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11610.16904090760844243No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11600.1688953082048176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10710.15593696128220658No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10430.151860177980711No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10150.1477833946792154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9530.13875623165447515No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9130.13293225550948143No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8860.12900107161161067No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8850.12885547220798582No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8380.12201230023761822No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8170.11895471276149652No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7870.11458673065275124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7690.11196594138750408No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7120.10366677538088803No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT7050.10264757955551415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.029.6368181
CCTCGTA454.005169E-628.77777929
CTCGTAA509.084135E-625.89999830
GTCCTAG451.3224545E-424.6666681
CTACACT451.3224545E-424.6666684
AGTCGGT2050.024.36585411
CCGCTTA701.92058E-723.78571325
TAGTTCG551.9012132E-523.5454547
TAGTACA551.9012132E-523.5454544
GCATAAC400.001930400423.12511
CTAACGT400.001930400423.1254
CGTAAAA400.001930400423.12532
CGTCCGT400.001930400423.1258
TATACAC1850.023.037
CAGTCGG2250.022.210
GTCGGTG2250.022.212
GCAGTCG2450.021.1428599
GTATAGT705.096992E-621.1428571
ATACGGC806.951723E-720.812529
ATATCCA450.00382426320.5555574
Produced by FastQC (version 0.11.5)