##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631680.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686816
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.547632844895865	34.0	31.0	34.0	31.0	34.0
2	32.72127178167078	34.0	31.0	34.0	31.0	34.0
3	32.80607033033593	34.0	31.0	34.0	31.0	34.0
4	36.25150112985137	37.0	37.0	37.0	35.0	37.0
5	36.160917916880216	37.0	35.0	37.0	35.0	37.0
6	36.217872035596145	37.0	36.0	37.0	35.0	37.0
7	36.207783452918974	37.0	36.0	37.0	35.0	37.0
8	36.1850015142338	37.0	36.0	37.0	35.0	37.0
9	38.019367632670175	39.0	38.0	39.0	35.0	39.0
10	37.93959954340027	39.0	38.0	39.0	35.0	39.0
11	38.02010290965848	39.0	38.0	39.0	35.0	39.0
12	37.94912320039137	39.0	38.0	39.0	35.0	39.0
13	38.006276790290265	39.0	38.0	39.0	35.0	39.0
14	39.375709069095656	40.0	39.0	41.0	37.0	41.0
15	39.37849292969296	40.0	39.0	41.0	37.0	41.0
16	39.32827569538275	40.0	39.0	41.0	36.0	41.0
17	39.303555828635325	40.0	39.0	41.0	36.0	41.0
18	39.32356118669338	40.0	39.0	41.0	36.0	41.0
19	39.34963803988259	40.0	39.0	41.0	36.0	41.0
20	39.33818082281135	40.0	39.0	41.0	36.0	41.0
21	39.30758602012766	40.0	39.0	41.0	36.0	41.0
22	39.281884813399806	40.0	39.0	41.0	36.0	41.0
23	39.21466739272236	40.0	39.0	41.0	36.0	41.0
24	39.234412127847925	40.0	39.0	41.0	36.0	41.0
25	39.1816075921353	40.0	39.0	41.0	36.0	41.0
26	39.1173239994409	40.0	39.0	41.0	36.0	41.0
27	39.01127812980478	40.0	39.0	41.0	35.0	41.0
28	38.99055642268089	40.0	39.0	41.0	35.0	41.0
29	38.96755026091413	40.0	38.0	41.0	35.0	41.0
30	38.92480809998602	40.0	38.0	41.0	35.0	41.0
31	38.83878797232447	40.0	38.0	41.0	35.0	41.0
32	38.799267926198574	40.0	38.0	41.0	35.0	41.0
33	38.7441396240041	40.0	38.0	41.0	35.0	41.0
34	38.71130841448073	40.0	38.0	41.0	35.0	41.0
35	38.64401237012533	40.0	38.0	41.0	35.0	41.0
36	38.60161964776592	40.0	38.0	41.0	35.0	41.0
37	38.55579805945115	40.0	38.0	41.0	35.0	41.0
38	38.51166105623631	40.0	38.0	41.0	35.0	41.0
39	38.44093905791362	40.0	38.0	41.0	34.0	41.0
40	38.145073789777754	40.0	38.0	41.0	34.0	41.0
41	38.209521909798255	40.0	37.0	41.0	34.0	41.0
42	38.19226546848064	40.0	37.0	41.0	34.0	41.0
43	37.71181946838746	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	4.0
18	4.0
19	6.0
20	27.0
21	56.0
22	109.0
23	200.0
24	383.0
25	580.0
26	905.0
27	1509.0
28	2247.0
29	3401.0
30	4819.0
31	6511.0
32	9007.0
33	12436.0
34	18121.0
35	27006.0
36	41669.0
37	74583.0
38	168783.0
39	314449.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.63631016167358	16.796638400969112	12.89952476354657	27.667526673810745
2	20.240501094907515	19.776912593766017	33.22665750361086	26.755928807715602
3	20.56198457811117	20.22492195871966	29.87656082560686	29.336532637562318
4	15.099968550528816	16.141004286446442	34.30423868983833	34.45478847318641
5	16.331739505194985	33.47024530587523	33.86088850580068	16.337126683129107
6	33.58192004845548	34.564861622326795	15.588308950286539	16.264909378931183
7	29.81744746773517	28.588006103526997	20.170758980571215	21.42378744816661
8	27.2206821040861	31.648068769510317	20.23307552532265	20.89817360108093
9	28.180182173973815	13.19480035409775	17.878005171690816	40.747012300237614
10	18.878564273400738	24.061903042445138	30.130194986721335	26.929337697432793
11	38.54685971206262	19.98905092484741	19.21577249219587	22.248316870894097
12	22.611441783534456	24.32106998089736	27.0149501467642	26.05253808880399
13	34.22095583096492	17.986913525602198	21.576521222569074	26.21560942086381
14	23.78715696780506	20.351447840469643	23.751339514513347	32.110055677211946
15	28.890561664259423	24.779853701719237	20.61076037832549	25.71882425569585
16	25.24591739272236	24.678807715603597	22.754565997297675	27.320708894376367
17	26.342572100824675	24.335629921259844	22.305391837115035	27.01640614080045
18	26.16566882542049	22.020308204817592	23.999295298886455	27.81472767087546
19	27.599677351721567	22.88647905698178	23.835641569212132	25.678202022084516
20	29.19748520710059	21.424806643991985	23.512702091972233	25.86500605693519
21	26.951032008572895	23.030913665377625	22.368873177095466	27.649181148954018
22	27.709022503843823	23.63238480175185	21.966873223687276	26.691719470717047
23	27.200589386385875	22.849788007268323	22.985195452639427	26.964427153706378
24	27.301635372501515	22.333783720821877	23.364918697293017	26.99966220938359
25	27.215586124959234	22.602851418720586	23.624959232166983	26.556603224153193
26	27.192872617993757	23.317162092904066	22.905698178260263	26.584267110841914
27	26.66405558402833	22.384452313283322	24.361546615105066	26.589945487583282
28	25.84520453804221	23.39141778875274	22.951416390998464	27.81196128220659
29	26.58979988817966	23.954450682570005	23.1022573731538	26.353492056096538
30	26.22521898150305	23.056102362204726	24.146059497740296	26.572619158551923
31	27.02223011694544	23.09381260774356	23.371470670456134	26.512486604854868
32	24.897352420444484	23.108518147509667	23.643595955830964	28.350533476214885
33	25.242568606438986	22.548834039975773	25.210245538834275	26.998351814750965
34	25.6247670409542	22.452738433583377	24.754519405488516	27.16797511997391
35	25.304011554768675	23.799969715324046	25.062607743558686	25.8334109863486
36	25.37855844942459	22.921714112658993	25.441748590597772	26.25797884731864
37	25.97769999534082	22.44079928248614	25.085175651120533	26.496325071052507
38	25.10672436285701	21.89363672366398	26.104808274705306	26.894830638773705
39	24.599310441224436	21.147585379490287	27.078577086148254	27.174527093137023
40	24.322089176722734	22.019289008992217	27.734793598285425	25.923828215999627
41	22.84818641382845	21.147585379490287	28.837417881936357	27.16681032474491
42	21.65762009038811	21.435872198667475	29.5374598145646	27.36904789637982
43	20.89817360108093	21.922902203792574	29.439325816521457	27.73959837860504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	9.0
2	11.0
3	27.0
4	43.0
5	43.0
6	54.0
7	65.0
8	76.0
9	87.0
10	149.5
11	212.0
12	212.0
13	414.0
14	616.0
15	1257.5
16	1899.0
17	1865.0
18	1831.0
19	1831.0
20	2093.5
21	2356.0
22	2148.0
23	1940.0
24	2199.5
25	2459.0
26	2459.0
27	2841.5
28	3224.0
29	4345.5
30	5467.0
31	6398.5
32	7330.0
33	7330.0
34	9000.0
35	10670.0
36	12155.5
37	13641.0
38	16712.5
39	19784.0
40	19784.0
41	23078.0
42	26372.0
43	29621.0
44	32870.0
45	42293.5
46	51717.0
47	51717.0
48	57105.0
49	62493.0
50	69454.0
51	76415.0
52	78043.0
53	79671.0
54	79671.0
55	72096.5
56	64522.0
57	60801.0
58	57080.0
59	51655.0
60	46230.0
61	46230.0
62	41837.0
63	37444.0
64	31216.0
65	24988.0
66	21579.5
67	18171.0
68	18171.0
69	15562.0
70	12953.0
71	11127.5
72	9302.0
73	7526.0
74	5750.0
75	5750.0
76	4643.0
77	3536.0
78	2905.5
79	2275.0
80	1842.5
81	1410.0
82	1410.0
83	1130.0
84	850.0
85	705.0
86	560.0
87	439.5
88	319.0
89	319.0
90	252.5
91	186.0
92	116.0
93	46.0
94	28.5
95	11.0
96	11.0
97	7.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	686816.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.621992887347815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.41981154022544	42.883232735750056
2	6.493521877551191	6.444429928433652
3	2.103640184511849	3.1316045482015804
4	1.0732781511204825	2.1303280312418558
5	0.6644488295965827	1.6485637548124106
6	0.45465019887730174	1.3536389360952443
7	0.33858249586487454	1.1760796741111093
8	0.26370820126161043	1.0468581189871138
9	0.2098788565411822	0.9373146413842113
>10	1.6367237813246105	16.63105450048287
>50	0.20098079925151174	6.859888862819343
>100	0.13015054924146	11.747569792039963
>500	0.008263526935965482	2.666367518152863
>1k	0.0023610076959901384	1.3430689574877743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1315	0.19146321576666822	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1239	0.18039766109118016	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1167	0.1699145040301915	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1161	0.16904090760844243	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1160	0.1688953082048176	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1071	0.15593696128220658	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1043	0.151860177980711	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1015	0.1477833946792154	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	953	0.13875623165447515	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	913	0.13293225550948143	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	886	0.12900107161161067	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	885	0.12885547220798582	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	838	0.12201230023761822	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	817	0.11895471276149652	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	787	0.11458673065275124	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	769	0.11196594138750408	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	712	0.10366677538088803	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	705	0.10264757955551415	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.4559940362484275E-4	0.0
5	0.0	0.0	0.0	1.4559940362484275E-4	0.0
6	0.0	0.0	0.0	1.4559940362484275E-4	0.0
7	0.0	0.0	0.0	1.4559940362484275E-4	0.0
8	1.4559940362484275E-4	0.0	0.0	1.4559940362484275E-4	0.0
9	1.4559940362484275E-4	0.0	0.0	1.4559940362484275E-4	0.0
10	1.4559940362484275E-4	0.0	0.0	1.4559940362484275E-4	0.0
11	1.4559940362484275E-4	0.0	0.0	1.4559940362484275E-4	0.0
12	1.4559940362484275E-4	0.0	0.0	2.911988072496855E-4	0.0
13	1.4559940362484275E-4	0.0	0.0	2.911988072496855E-4	0.0
14	1.4559940362484275E-4	0.0	0.0	2.911988072496855E-4	0.0
15	1.4559940362484275E-4	0.0	0.0	2.911988072496855E-4	0.0
16	1.4559940362484275E-4	0.0	0.0	7.279970181242138E-4	0.0
17	2.911988072496855E-4	0.0	0.0	0.0016015934398732703	0.0
18	5.82397614499371E-4	0.0	0.0	0.001747192843498113	0.0
19	7.279970181242138E-4	0.0	0.0	0.0020383916507477986	0.0
20	7.279970181242138E-4	0.0	0.0	0.0033487862833713835	0.0
21	7.279970181242138E-4	0.0	0.0	0.006406373759493081	0.0
22	7.279970181242138E-4	0.0	0.0	0.01004635885011415	0.0
23	7.279970181242138E-4	0.0	0.0	0.01164795228998742	0.0
24	7.279970181242138E-4	0.0	0.0	0.014559940362484276	0.0
25	7.279970181242138E-4	0.0	0.0	0.016161533802357547	0.0
26	7.279970181242138E-4	0.0	0.0	0.019364720682104087	0.0
27	7.279970181242138E-4	0.0	0.0	0.03858384196058333	0.0
28	7.279970181242138E-4	0.0	0.0	0.12434189069561571	0.0
29	7.279970181242138E-4	1.4559940362484275E-4	0.0	0.2754740716582025	0.0
30	7.279970181242138E-4	1.4559940362484275E-4	0.0	0.471305269533616	0.0
31	7.279970181242138E-4	1.4559940362484275E-4	0.0	1.054722079858361	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1005	0.0	29.636818	1
CCTCGTA	45	4.005169E-6	28.777779	29
CTCGTAA	50	9.084135E-6	25.899998	30
GTCCTAG	45	1.3224545E-4	24.666668	1
CTACACT	45	1.3224545E-4	24.666668	4
AGTCGGT	205	0.0	24.365854	11
CCGCTTA	70	1.92058E-7	23.785713	25
TAGTTCG	55	1.9012132E-5	23.545454	7
TAGTACA	55	1.9012132E-5	23.545454	4
GCATAAC	40	0.0019304004	23.125	11
CTAACGT	40	0.0019304004	23.125	4
CGTAAAA	40	0.0019304004	23.125	32
CGTCCGT	40	0.0019304004	23.125	8
TATACAC	185	0.0	23.0	37
CAGTCGG	225	0.0	22.2	10
GTCGGTG	225	0.0	22.2	12
GCAGTCG	245	0.0	21.142859	9
GTATAGT	70	5.096992E-6	21.142857	1
ATACGGC	80	6.951723E-7	20.8125	29
ATATCCA	45	0.003824263	20.555557	4
>>END_MODULE