FastQCFastQC Report
Fri 10 Feb 2017
ERR1631679.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631679.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215329
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4970.2308095983355702No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC4730.2196638632046775No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4510.20944693933469247No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4330.20108763798652296No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4120.19133511974699183No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3970.18436903529018386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3800.17647413957246819No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.16857924385475248No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT3630.16857924385475248No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3550.16486399881112157No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3500.16254197065885226No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3500.16254197065885226No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3470.1611487537674907No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3140.14582336796251316No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3030.14071490602752068No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2730.12678273711390478No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2580.11981665265709682No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2490.11563700198301205No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2440.11331497383074274No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2420.112386162569835No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2250.1044912668521193No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2220.10309804996075772No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2210.10263364433030386No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG2180.10124042743894227No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2160.10031161617803455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTC200.00183861637.018
GGTAAGT250.00548654929.61
GACGTAT250.00548654929.61
ACGTATA250.00548654929.62
GTAAGTT250.00548654929.62
GGTATCA2950.029.4745771
ATTAGAG358.8459055E-426.4285723
ACGATTA451.3183389E-424.66666623
AACGATT451.3183389E-424.66666622
ATCCTAT502.6922306E-422.21
TAAGTCT502.6922306E-422.235
CCACGTG1250.022.1999996
AAGTCTG603.7082114E-521.58333436
CGTGTCC1400.021.1428599
CCAGGAC1500.020.9666673
ATCTAAG1151.3278623E-1020.91304432
CATCTAA1151.3278623E-1020.91304431
TGAAACA1151.3278623E-1020.91304426
ACATCTA1151.3278623E-1020.91304430
GACAGGC1251.8189894E-1120.727
Produced by FastQC (version 0.11.5)