FastQCFastQC Report
Fri 10 Feb 2017
ERR1631678.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631678.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61062
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1900.3111591497166814No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1710.28004323474501325No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1430.23418820215518654No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1430.23418820215518654No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1410.23091284268448461No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1400.22927516294913367No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1320.21617372506632604No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1220.19979692771281646No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1200.19652156824211459No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1170.1916085290360617No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1140.18669548983000886No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1140.18669548983000886No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1120.18342013035930693No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1100.18014477088860503No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1090.17850709115325408No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1080.1768694114179031No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1080.1768694114179031No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA980.16049261406439358No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA960.15721725459369165No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC950.1555795748583407No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC860.1408404572401821No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA850.13920277750483115No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA830.13592741803412925No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA810.13265205856342735No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG810.13265205856342735No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA780.12773901935737447No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT780.12773901935737447No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA780.12773901935737447No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC770.1261013396220235No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG760.12446365988667257No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA750.12282598015132161No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT730.1195506206806197No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC730.1195506206806197No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT730.1195506206806197No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG730.1195506206806197No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT710.1162752612099178No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC710.1162752612099178No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA690.11299990173921587No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA690.11299990173921587No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT690.11299990173921587No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC680.11136222200386493No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC660.10808686253316302No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT650.10644918279781206No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC640.10481150306246109No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG630.10317382332711016No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT620.10153614359175919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA900.030.8333321
TACCACG854.9813025E-821.7647064
TCTTATA1251.6370905E-1120.7237
ACCACGT909.115138E-820.5555555
GGATACC909.115138E-820.5555551
CTTATAC555.0668477E-420.18181837
CGCCCTA951.6122249E-719.47368416
ATCGAGC951.6122249E-719.47368426
TCGAGCT951.6122249E-719.47368427
GCCCTAC1002.7658825E-718.517
CCTACTC1002.7658825E-718.519
AATCTGC500.00696071118.56
GATACCA1002.7658825E-718.52
CCACGTG1002.7658825E-718.56
TGTCCCG1002.7658825E-718.511
GAGCTCA902.0851621E-618.529
TCTGCGA500.00696071118.58
TCCCGCC1002.7658825E-718.513
TCATCGA1002.7658825E-718.524
ATCTGCG500.00696071118.57
Produced by FastQC (version 0.11.5)