##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631678.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61062
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03710982280305	33.0	31.0	34.0	30.0	34.0
2	32.2263273394255	33.0	31.0	34.0	30.0	34.0
3	32.30154596967017	34.0	31.0	34.0	30.0	34.0
4	35.91235138056402	37.0	35.0	37.0	35.0	37.0
5	35.698830696668956	37.0	35.0	37.0	33.0	37.0
6	35.724165602174835	37.0	35.0	37.0	35.0	37.0
7	35.74820674068979	37.0	35.0	37.0	35.0	37.0
8	35.73368052143723	37.0	35.0	37.0	35.0	37.0
9	37.48906029936786	39.0	37.0	39.0	35.0	39.0
10	37.35527824178703	39.0	37.0	39.0	34.0	39.0
11	37.423553109953815	39.0	37.0	39.0	35.0	39.0
12	37.338901444433525	39.0	37.0	39.0	35.0	39.0
13	37.417657462906554	39.0	37.0	39.0	35.0	39.0
14	38.60523729979365	40.0	38.0	41.0	35.0	41.0
15	38.57855949690479	40.0	38.0	41.0	35.0	41.0
16	38.508466804231766	40.0	38.0	41.0	34.0	41.0
17	38.49993449281059	40.0	38.0	41.0	34.0	41.0
18	38.502178114048014	40.0	38.0	41.0	34.0	41.0
19	38.5114473813501	40.0	38.0	41.0	34.0	41.0
20	38.504356228096036	40.0	38.0	41.0	34.0	41.0
21	38.47073466312928	40.0	38.0	41.0	34.0	41.0
22	38.440290196849105	40.0	38.0	41.0	34.0	41.0
23	38.34864563885886	40.0	38.0	41.0	34.0	41.0
24	38.38726212701844	40.0	38.0	41.0	34.0	41.0
25	38.31982247551669	40.0	38.0	41.0	34.0	41.0
26	38.19756968327274	40.0	38.0	41.0	34.0	41.0
27	38.0706986341751	40.0	38.0	41.0	33.0	41.0
28	38.07266384985752	40.0	38.0	41.0	33.0	41.0
29	38.01858766499623	40.0	38.0	41.0	33.0	41.0
30	37.97826798991189	40.0	37.0	41.0	33.0	41.0
31	37.90426124267138	40.0	37.0	41.0	33.0	41.0
32	37.86284432216436	40.0	37.0	41.0	33.0	41.0
33	37.775097441944254	40.0	37.0	41.0	33.0	41.0
34	37.762503684779404	40.0	37.0	41.0	33.0	41.0
35	37.69119910910222	40.0	37.0	41.0	33.0	41.0
36	37.61891192558383	39.0	37.0	41.0	33.0	41.0
37	37.580934132521044	39.0	37.0	41.0	33.0	41.0
38	37.5205365038813	39.0	36.0	41.0	33.0	41.0
39	37.46117061347483	39.0	36.0	41.0	32.0	41.0
40	37.17727883135174	39.0	36.0	40.0	32.0	41.0
41	37.25318528708526	39.0	36.0	40.0	32.0	41.0
42	37.207150109724545	39.0	36.0	40.0	32.0	41.0
43	36.68510694048672	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	3.0
20	8.0
21	9.0
22	23.0
23	30.0
24	60.0
25	114.0
26	161.0
27	241.0
28	315.0
29	530.0
30	732.0
31	990.0
32	1265.0
33	1736.0
34	2494.0
35	3713.0
36	5918.0
37	10170.0
38	19134.0
39	13411.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.99993449281059	16.98765189479545	13.356915921522386	27.655497690871574
2	21.412662539713732	20.15328682322885	32.11162425076152	26.322426386295895
3	21.103141069732402	20.08941731355016	29.704235039795616	29.10320657692182
4	15.982116537289967	16.6421014706364	34.63365104320199	32.74213094887164
5	16.409550948216566	33.35298549015754	33.59699977072484	16.64046379090105
6	32.73885558940094	34.36670924633979	16.2981887262127	16.596246438046574
7	29.14578625004094	28.171366807507127	20.127083947463234	22.5557629949887
8	26.72529560119223	30.7064950378304	21.250532245913988	21.317677115063375
9	28.40227965019161	12.6920179489699	17.595231076610656	41.310471324227834
10	19.661982902623564	23.48268972519734	29.068815302479443	27.786512069699647
11	39.258458615833085	19.555533719825753	18.777635845534046	22.40837181880711
12	22.206937211358944	24.075529789394388	26.664701450984246	27.052831548262425
13	35.71124430906292	17.333202318954505	20.872228227047916	26.083325144934655
14	23.888834299564376	20.577445874684745	23.84625462644525	31.687465199305624
15	30.23320559431398	24.01657331892175	19.89944646424945	25.850774622514823
16	24.75189152009433	24.49313812190888	22.619632504667386	28.1353378533294
17	26.733483999868984	24.159051455897284	21.440503095214698	27.666961449019027
18	26.71055648357407	21.22432937014837	23.029052438505126	29.036061707772426
19	28.06983066391536	22.50663260292817	23.166617536274607	26.256919196881856
20	30.49359667223478	20.22534473158429	23.05689299400609	26.224165602174835
21	27.617831056958504	22.016966362058234	21.589531951131637	28.775670629851625
22	28.859192296354525	22.850545347351872	20.46444597294553	27.825816383348073
23	28.331859421571515	22.24951688447807	21.95637221185025	27.462251482100157
24	27.6980773639907	21.420850938390487	22.880023582588187	28.001048115030624
25	27.906062690380267	21.794241918050506	23.441747731813567	26.85794765975566
26	28.125511774917296	22.837443909469062	22.560676034194753	26.47636828141889
27	26.756411516163897	21.68287969604664	24.910746454423375	26.64996233336609
28	26.289672791588874	23.428646293930758	21.581343552454882	28.70033736202548
29	27.03973011037961	23.63663162032033	22.174183616651927	27.14945465264813
30	26.803904228489078	22.19711113294684	23.634993940584977	27.3639906979791
31	28.369526055484588	22.35269070780518	22.703154171170286	26.574629065539945
32	25.32016638826111	22.483705086633258	22.763748321378273	29.43238020372736
33	25.574006747240507	21.859749107464545	24.953326127542496	27.612918017752445
34	25.844223903573415	22.151256100357013	23.458124529167076	28.546395466902492
35	25.718122563951397	24.22128328584062	24.763355278241786	25.2972388719662
36	26.063672988110447	23.00121188300416	24.696210409092398	26.238904719793
37	26.869411417903116	22.902951098883104	24.05915299204088	26.168484491172904
38	25.415151812911468	22.008777963381483	25.393861976351907	27.18220824735515
39	25.223543283875404	20.70190953457142	26.312600307883788	27.761946873669384
40	24.745340801152928	22.362516786217288	27.22478792047427	25.667354492155514
41	23.354950705839965	20.885329664930726	28.186105925125283	27.573613704104027
42	21.895778061642265	21.248894566178638	29.221119517867084	27.63420785431201
43	20.665880580393697	22.373980544364745	28.970554518358387	27.989584356883167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	2.5
4	4.0
5	4.0
6	4.5
7	5.0
8	5.5
9	6.0
10	9.5
11	13.0
12	13.0
13	27.0
14	41.0
15	68.5
16	96.0
17	99.0
18	102.0
19	102.0
20	114.0
21	126.0
22	103.5
23	81.0
24	88.0
25	95.0
26	95.0
27	112.5
28	130.0
29	185.5
30	241.0
31	295.0
32	349.0
33	349.0
34	460.0
35	571.0
36	625.0
37	679.0
38	911.5
39	1144.0
40	1144.0
41	1408.5
42	1673.0
43	2037.5
44	2402.0
45	3743.0
46	5084.0
47	5084.0
48	5748.0
49	6412.0
50	7084.0
51	7756.0
52	7886.0
53	8016.0
54	8016.0
55	7093.5
56	6171.0
57	5862.5
58	5554.0
59	5016.5
60	4479.0
61	4479.0
62	3937.5
63	3396.0
64	2765.5
65	2135.0
66	1870.0
67	1605.0
68	1605.0
69	1326.5
70	1048.0
71	883.0
72	718.0
73	542.0
74	366.0
75	366.0
76	287.5
77	209.0
78	182.0
79	155.0
80	121.0
81	87.0
82	87.0
83	68.5
84	50.0
85	40.0
86	30.0
87	23.5
88	17.0
89	17.0
90	15.5
91	14.0
92	7.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	61062.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.99515246798336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.05068760059865	49.32527594903541
2	6.63598113687064	7.697094756149488
3	2.6346257024256627	4.583865579247322
4	1.349786801457092	3.1312436539910253
5	0.960099398525965	2.784055550096623
6	0.5732358173552086	1.994693917657463
7	0.46593059046113006	1.891520094330353
8	0.3868635811707565	1.7948969899446463
9	0.24284867139186175	1.267564115161639
>10	1.5107446417982098	16.42920310504078
>50	0.14119108801852429	5.507516949985261
>100	0.04800496992629825	3.593069339359995
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	190	0.3111591497166814	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	171	0.28004323474501325	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	143	0.23418820215518654	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	143	0.23418820215518654	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	141	0.23091284268448461	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	140	0.22927516294913367	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	132	0.21617372506632604	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	122	0.19979692771281646	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	120	0.19652156824211459	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	117	0.1916085290360617	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	114	0.18669548983000886	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	114	0.18669548983000886	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	112	0.18342013035930693	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	110	0.18014477088860503	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	109	0.17850709115325408	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	108	0.1768694114179031	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	108	0.1768694114179031	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	98	0.16049261406439358	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	96	0.15721725459369165	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	95	0.1555795748583407	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	86	0.1408404572401821	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	85	0.13920277750483115	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	83	0.13592741803412925	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	81	0.13265205856342735	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	81	0.13265205856342735	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	78	0.12773901935737447	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	78	0.12773901935737447	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	78	0.12773901935737447	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	77	0.1261013396220235	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	76	0.12446365988667257	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	75	0.12282598015132161	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	73	0.1195506206806197	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	73	0.1195506206806197	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	73	0.1195506206806197	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	73	0.1195506206806197	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	71	0.1162752612099178	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	71	0.1162752612099178	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	69	0.11299990173921587	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	69	0.11299990173921587	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	69	0.11299990173921587	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	68	0.11136222200386493	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	66	0.10808686253316302	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	65	0.10644918279781206	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	64	0.10481150306246109	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	63	0.10317382332711016	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	62	0.10153614359175919	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0016376797353509548	0.0
20	0.0	0.0	0.0	0.0032753594707019096	0.0
21	0.0	0.0	0.0	0.009826078412105728	0.0
22	0.0	0.0	0.0	0.013101437882807638	0.0
23	0.0	0.0	0.0	0.016376797353509547	0.0
24	0.0	0.0	0.0	0.018014477088860503	0.0
25	0.0	0.0	0.0	0.019652156824211457	0.0
26	0.0	0.0	0.0	0.021289836559562413	0.0
27	0.0	0.0	0.0	0.036028954177721007	0.0
28	0.0	0.0	0.0	0.09989846385640824	0.0
29	0.0	0.0	0.0	0.23091284268448461	0.0
30	0.0	0.0	0.0	0.4323474501326521	0.0
31	0.0	0.0	0.0	0.9187383315318857	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	90	0.0	30.833332	1
TACCACG	85	4.9813025E-8	21.764706	4
TCTTATA	125	1.6370905E-11	20.72	37
ACCACGT	90	9.115138E-8	20.555555	5
GGATACC	90	9.115138E-8	20.555555	1
CTTATAC	55	5.0668477E-4	20.181818	37
CGCCCTA	95	1.6122249E-7	19.473684	16
ATCGAGC	95	1.6122249E-7	19.473684	26
TCGAGCT	95	1.6122249E-7	19.473684	27
GCCCTAC	100	2.7658825E-7	18.5	17
CCTACTC	100	2.7658825E-7	18.5	19
AATCTGC	50	0.006960711	18.5	6
GATACCA	100	2.7658825E-7	18.5	2
CCACGTG	100	2.7658825E-7	18.5	6
TGTCCCG	100	2.7658825E-7	18.5	11
GAGCTCA	90	2.0851621E-6	18.5	29
TCTGCGA	50	0.006960711	18.5	8
TCCCGCC	100	2.7658825E-7	18.5	13
TCATCGA	100	2.7658825E-7	18.5	24
ATCTGCG	50	0.006960711	18.5	7
>>END_MODULE