FastQCFastQC Report
Fri 10 Feb 2017
ERR1631677.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631677.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372367
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.22343548166190882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7450.20007143490158902No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7070.18986644896029992No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6950.18664382182094547No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6940.1863752695593326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6280.1686508202928831No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6160.16542819315352864No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5940.15952004339804549No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5750.15441755042740093No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5640.15146347554965933No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5280.14179559413159598No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4910.13185916045191975No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4900.13159060819030688No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4750.1275623242661138No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4650.12487680164998509No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4560.12245983129546925No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4500.12084851772579201No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4450.11950575641772768No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4200.1127919498774059No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4140.11118063630772866No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3900.10473538202901975No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3890.10446682976740689No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3810.10231841167450391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4200.032.5952381
CTAGCAC358.858091E-426.4285723
TTGGTTA551.8981837E-523.5454544
TCTAGCA400.001928749523.1252
CTCGTCC502.6973503E-422.226
TAGCACT502.6973503E-422.24
TACACCA957.1049726E-921.4210535
ATACACA1400.021.14285937
ATCTAGC450.003821025820.5555531
TGGACCG450.003821025820.5555535
TGCGCTA450.003821025820.5555535
ACGTGTG450.003821025820.5555539
GGACCGT450.003821025820.5555536
CAGTCGG1001.2827513E-820.3510
GACACTT555.134124E-420.18181824
TCCCTCG555.134124E-420.18181823
TTACATG555.134124E-420.1818188
GCGGTAA1850.020.023
AGATTAT656.8861234E-519.92307723
AGCCGCG2050.018.95122119
Produced by FastQC (version 0.11.5)