##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631677.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	372367
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.639785480453426	34.0	31.0	34.0	31.0	34.0
2	32.81068139765339	34.0	31.0	34.0	31.0	34.0
3	32.89113428418737	34.0	31.0	34.0	31.0	34.0
4	36.300856413162286	37.0	37.0	37.0	35.0	37.0
5	36.22937048664355	37.0	35.0	37.0	35.0	37.0
6	36.2878719113133	37.0	37.0	37.0	35.0	37.0
7	36.28421154398752	37.0	37.0	37.0	35.0	37.0
8	36.25981625654261	37.0	37.0	37.0	35.0	37.0
9	38.096617047160464	39.0	38.0	39.0	37.0	39.0
10	38.04081725824254	39.0	38.0	39.0	35.0	39.0
11	38.10617213662865	39.0	38.0	39.0	37.0	39.0
12	38.04481331589534	39.0	38.0	39.0	35.0	39.0
13	38.09159780539092	39.0	38.0	39.0	37.0	39.0
14	39.49310492068309	41.0	39.0	41.0	37.0	41.0
15	39.50148106572279	41.0	39.0	41.0	37.0	41.0
16	39.448570899139824	41.0	39.0	41.0	37.0	41.0
17	39.42899343926825	41.0	39.0	41.0	37.0	41.0
18	39.44851450316489	41.0	39.0	41.0	37.0	41.0
19	39.47676888660918	41.0	39.0	41.0	37.0	41.0
20	39.47595785877911	41.0	39.0	41.0	37.0	41.0
21	39.431764898608094	41.0	39.0	41.0	37.0	41.0
22	39.421369240561056	40.0	39.0	41.0	37.0	41.0
23	39.360214519546574	40.0	39.0	41.0	36.0	41.0
24	39.367615819876626	41.0	39.0	41.0	36.0	41.0
25	39.32035599287799	41.0	39.0	41.0	36.0	41.0
26	39.2574341979821	40.0	39.0	41.0	36.0	41.0
27	39.15211605754538	40.0	39.0	41.0	36.0	41.0
28	39.1420802595289	40.0	39.0	41.0	36.0	41.0
29	39.132659446191525	40.0	39.0	41.0	35.0	41.0
30	39.08389035548263	40.0	39.0	41.0	35.0	41.0
31	39.0018476395599	40.0	39.0	41.0	35.0	41.0
32	38.95339006947447	40.0	38.0	41.0	35.0	41.0
33	38.90693858478329	40.0	38.0	41.0	35.0	41.0
34	38.862495871008974	40.0	38.0	41.0	35.0	41.0
35	38.78545359819748	40.0	38.0	41.0	35.0	41.0
36	38.73598358608577	40.0	38.0	41.0	35.0	41.0
37	38.69585382163296	40.0	38.0	41.0	35.0	41.0
38	38.65214962657808	40.0	38.0	41.0	35.0	41.0
39	38.596003405242676	40.0	38.0	41.0	35.0	41.0
40	38.30190108145996	40.0	38.0	41.0	34.0	41.0
41	38.369463459436524	40.0	38.0	41.0	34.0	41.0
42	38.3406209465393	40.0	38.0	41.0	34.0	41.0
43	37.888497100978334	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	3.0
20	7.0
21	20.0
22	40.0
23	102.0
24	160.0
25	256.0
26	451.0
27	731.0
28	1103.0
29	1684.0
30	2413.0
31	3383.0
32	4364.0
33	5961.0
34	9130.0
35	13418.0
36	20621.0
37	37385.0
38	84919.0
39	186213.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.36358216490721	16.921478004227012	13.528857283271611	27.186082547594175
2	19.664470804340876	20.207483477322103	34.16387596108141	25.96416975725561
3	20.621053960205927	20.611923183311088	30.21427784954091	28.552745006942075
4	15.272835670185597	16.657491131061562	34.03013693479819	34.039536263954645
5	16.150195908874846	33.73875773094823	33.913316700996596	16.197729659180325
6	32.98627429390897	34.1679042450056	16.262987858752254	16.58283360233318
7	29.38552557020359	28.782088638359465	20.59527294309109	21.237112848345852
8	26.45615750053039	32.144094401491	20.73357735782172	20.66617074015689
9	27.86954805339893	13.751218555887068	18.36467785813458	40.014555532579415
10	18.92407221907419	24.641012764288995	30.281147362682514	26.153767653954297
11	37.251152760582976	20.705379370352368	19.93866266344762	22.10480520561704
12	22.07929274076382	24.660079974863507	27.35097363622448	25.909653648148197
13	33.47987335075342	18.35500997671652	22.54254539204602	25.622571280484042
14	23.435481661908817	21.086992134104257	24.291089167407424	31.186437036579505
15	28.656137627663032	25.17999715334603	21.230130489543917	24.933734729447025
16	24.691769141733825	25.0604913969283	23.27596161851077	26.971777842827105
17	25.819420088246275	24.694454664349955	22.908582124624363	26.577543122779407
18	25.437807324494383	22.595450187583754	24.502976901819977	27.463765586101886
19	27.176146113914502	23.44595520011172	24.56501247425255	24.81288621172123
20	28.56885814263885	21.684789468454507	24.523923978225785	25.22242841068086
21	26.137385965995914	23.67073344308169	23.032653269489508	27.159227321432887
22	26.95378484129904	23.896048790574888	22.68944347914826	26.460722888977813
23	26.578348779564248	23.439241393571397	23.73867716526975	26.24373266159461
24	27.050732207741284	22.715761600786323	24.01125771080681	26.222248480665577
25	26.606278214771983	23.23111339082142	24.459739987700306	25.702868406706287
26	26.841261443683244	23.672881861174595	23.669927786296853	25.815928908845304
27	26.136580309211073	22.72032698923374	25.139714314104094	26.003378387451093
28	25.077141637148298	24.0214626967481	23.607355109341054	27.29404055676255
29	26.11590178506688	24.53493462095191	23.47093056044171	25.878233033539495
30	25.536366004506306	23.8775186845236	24.559641429020296	26.026473881949798
31	26.595536124307472	23.252329019488837	24.317407289045484	25.834727567158204
32	24.220191370341627	23.431721930246237	24.312573348336453	28.035513351075686
33	24.80026425542543	22.788270711421795	25.898374453160457	26.513090579992323
34	24.999798585803788	22.797401488316634	25.440492847110512	26.762307078769066
35	24.77233482021769	23.96318685597811	25.71925009466467	25.545228229139532
36	24.849409319300584	23.26951636423206	26.235138989223	25.645935327244356
37	25.595984606584366	22.520792658855378	25.662317015202746	26.220905719357518
38	24.581931266734163	21.98583655372254	26.73357198677649	26.698660192766816
39	24.197632980366144	21.27712713532617	27.77313779147991	26.752102092827773
40	23.76150410750684	22.193964556472512	28.48318997118434	25.561341364836306
41	22.388933498403457	21.4259050882597	29.43735615669487	26.74780525664197
42	21.520166932085818	21.68398381166967	29.84716690791612	26.948682348328397
43	20.469321932394653	22.640029863011492	29.583985691535503	27.306662513058356
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	7.5
2	8.0
3	21.5
4	35.0
5	35.0
6	45.0
7	55.0
8	73.5
9	92.0
10	128.0
11	164.0
12	164.0
13	345.0
14	526.0
15	1019.5
16	1513.0
17	1460.5
18	1408.0
19	1408.0
20	1580.5
21	1753.0
22	1551.0
23	1349.0
24	1429.0
25	1509.0
26	1509.0
27	1689.5
28	1870.0
29	2708.0
30	3546.0
31	3978.5
32	4411.0
33	4411.0
34	5281.5
35	6152.0
36	6726.5
37	7301.0
38	9413.0
39	11525.0
40	11525.0
41	13555.5
42	15586.0
43	17013.0
44	18440.0
45	24533.5
46	30627.0
47	30627.0
48	33685.0
49	36743.0
50	40600.5
51	44458.0
52	45051.5
53	45645.0
54	45645.0
55	39688.0
56	33731.0
57	31033.0
58	28335.0
59	25079.0
60	21823.0
61	21823.0
62	19621.5
63	17420.0
64	14415.5
65	11411.0
66	9854.5
67	8298.0
68	8298.0
69	7130.0
70	5962.0
71	5028.0
72	4094.0
73	3307.0
74	2520.0
75	2520.0
76	2028.5
77	1537.0
78	1282.0
79	1027.0
80	811.0
81	595.0
82	595.0
83	525.5
84	456.0
85	340.5
86	225.0
87	179.0
88	133.0
89	133.0
90	98.5
91	64.0
92	38.5
93	13.0
94	6.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	372367.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.57550204392258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.98978126387516	40.43431512232025
2	6.7290388377966375	6.402748019624567
3	2.3380685055392743	3.3370434889234426
4	1.2298341931816852	2.3403991668560455
5	0.8109425651338835	1.9290499832515449
6	0.5665670781122063	1.6172827909647909
7	0.40789903022069	1.3584200802184936
8	0.3177298298396972	1.2092924935162954
9	0.27117461005029375	1.161114139323689
>10	1.9609122457172616	18.896917472284603
>50	0.23614048898171033	7.668087951111692
>100	0.13566725208324226	11.70683752175603
>500	0.00624409946826638	1.9384917698485498
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	832	0.22343548166190882	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	745	0.20007143490158902	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	707	0.18986644896029992	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	695	0.18664382182094547	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	694	0.1863752695593326	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	628	0.1686508202928831	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	616	0.16542819315352864	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	594	0.15952004339804549	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	575	0.15441755042740093	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	564	0.15146347554965933	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	528	0.14179559413159598	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	491	0.13185916045191975	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	490	0.13159060819030688	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	475	0.1275623242661138	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	465	0.12487680164998509	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	456	0.12245983129546925	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	450	0.12084851772579201	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	445	0.11950575641772768	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	420	0.1127919498774059	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	414	0.11118063630772866	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	390	0.10473538202901975	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	389	0.10446682976740689	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	381	0.10231841167450391	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	2.6855226161287115E-4	0.0	0.0	2.6855226161287115E-4	0.0
12	2.6855226161287115E-4	0.0	0.0	2.6855226161287115E-4	0.0
13	2.6855226161287115E-4	0.0	0.0	5.371045232257423E-4	0.0
14	2.6855226161287115E-4	0.0	0.0	8.056567848386136E-4	0.0
15	2.6855226161287115E-4	0.0	0.0	8.056567848386136E-4	0.0
16	2.6855226161287115E-4	0.0	0.0	0.0016113135696772271	0.0
17	2.6855226161287115E-4	0.0	0.0	0.002148418092902969	0.0
18	5.371045232257423E-4	0.0	0.0	0.0024169703545158404	0.0
19	5.371045232257423E-4	0.0	0.0	0.0034911794009673254	0.0
20	5.371045232257423E-4	0.0	0.0	0.005639597493870295	0.0
21	5.371045232257423E-4	0.0	0.0	0.007250911063547522	0.0
22	0.0010742090464514846	0.0	0.0	0.009667881418063361	0.0
23	0.0010742090464514846	0.0	0.0	0.012084851772579203	0.0
24	0.0010742090464514846	0.0	0.0	0.01611313569677227	0.0
25	0.0010742090464514846	0.0	0.0	0.01799300152806237	0.0
26	0.0010742090464514846	0.0	0.0	0.021484180929029695	0.0
27	0.0010742090464514846	0.0	0.0	0.0456538844741881	0.0
28	0.0010742090464514846	0.0	0.0	0.14098993734675735	0.0
29	0.0010742090464514846	0.0	0.0	0.30400116014577017	0.0
30	0.0010742090464514846	0.0	0.0	0.5290479553773562	0.0
31	0.0010742090464514846	0.0	0.0	1.2224498948617897	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	420	0.0	32.595238	1
CTAGCAC	35	8.858091E-4	26.428572	3
TTGGTTA	55	1.8981837E-5	23.545454	4
TCTAGCA	40	0.0019287495	23.125	2
CTCGTCC	50	2.6973503E-4	22.2	26
TAGCACT	50	2.6973503E-4	22.2	4
TACACCA	95	7.1049726E-9	21.421053	5
ATACACA	140	0.0	21.142859	37
ATCTAGC	45	0.0038210258	20.555553	1
TGGACCG	45	0.0038210258	20.555553	5
TGCGCTA	45	0.0038210258	20.555553	5
ACGTGTG	45	0.0038210258	20.555553	9
GGACCGT	45	0.0038210258	20.555553	6
CAGTCGG	100	1.2827513E-8	20.35	10
GACACTT	55	5.134124E-4	20.181818	24
TCCCTCG	55	5.134124E-4	20.181818	23
TTACATG	55	5.134124E-4	20.181818	8
GCGGTAA	185	0.0	20.0	23
AGATTAT	65	6.8861234E-5	19.923077	23
AGCCGCG	205	0.0	18.951221	19
>>END_MODULE