FastQCFastQC Report
Fri 10 Feb 2017
ERR1631676.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631676.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158950
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32860.2835325078735062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25990.22425471331808966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24830.2142456533931576No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA21150.1824927736313042No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20550.17731567367013243No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA20250.17472712368954657No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA17650.15229302385780233No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA17270.14901419388239354No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC17040.14702963889727771No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16230.14004055394969583No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG15110.13037663402217525No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG15030.12968635402735235No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT14860.1282195090383537No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC14700.12683894904870788No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA13180.11372362914707278No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC13100.11303334915224988No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13010.11225678415807412No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12950.11173907416195694No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12910.1113939341645455No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA12360.10664825920013805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.031.7142831
ATACGGC901.4370016E-1024.66666629
TTTTACG802.7259375E-823.1250024
AACGACC1550.020.29032137
CTTATAC12450.019.76305237
TAGTTCC1600.019.656254
AACCGTC500.007034916418.516
TATTAGA701.2192086E-418.52
GCCTAGT1601.8189894E-1218.51
TACAGCG500.007034916418.55
AGTCGGT2900.018.511
CAGTCGG3000.018.49999810
GTATCAA26000.018.286542
GCAGTCG3050.018.1967229
GTATTAG2650.018.1509441
GCACCGT1552.0008883E-1117.9032256
TATACTC852.7231474E-517.4117645
CTAGTTC1603.45608E-1117.343753
CTCTATG4700.017.3191471
TACGGCT1301.3929821E-817.07692330
Produced by FastQC (version 0.11.5)