##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631676.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1158950
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15396781569524	33.0	31.0	34.0	30.0	34.0
2	32.34212778808404	34.0	31.0	34.0	31.0	34.0
3	32.42632296475258	34.0	31.0	34.0	30.0	34.0
4	36.01713878942146	37.0	35.0	37.0	35.0	37.0
5	35.821021614392336	37.0	35.0	37.0	35.0	37.0
6	35.85698951637258	37.0	35.0	37.0	35.0	37.0
7	35.849808878726435	37.0	35.0	37.0	35.0	37.0
8	35.81551749428362	37.0	35.0	37.0	35.0	37.0
9	37.58218991328358	39.0	37.0	39.0	35.0	39.0
10	37.45820527201346	39.0	37.0	39.0	35.0	39.0
11	37.54348505112386	39.0	37.0	39.0	35.0	39.0
12	37.47176237111178	39.0	37.0	39.0	35.0	39.0
13	37.53956253505328	39.0	37.0	39.0	35.0	39.0
14	38.76883817248371	40.0	38.0	41.0	36.0	41.0
15	38.751362008714786	40.0	38.0	41.0	35.0	41.0
16	38.700094913499285	40.0	38.0	41.0	35.0	41.0
17	38.66994262047543	40.0	38.0	41.0	35.0	41.0
18	38.69946934725398	40.0	38.0	41.0	35.0	41.0
19	38.721947452435394	40.0	38.0	41.0	35.0	41.0
20	38.709017645282366	40.0	38.0	41.0	35.0	41.0
21	38.67424392769317	40.0	38.0	41.0	35.0	41.0
22	38.63951076405367	40.0	38.0	41.0	35.0	41.0
23	38.580023296949825	40.0	38.0	41.0	35.0	41.0
24	38.58369558652228	40.0	38.0	41.0	35.0	41.0
25	38.52552137710859	40.0	38.0	41.0	34.0	41.0
26	38.437503774968725	40.0	38.0	41.0	34.0	41.0
27	38.32461797316537	40.0	38.0	41.0	34.0	41.0
28	38.30856723758575	40.0	38.0	41.0	34.0	41.0
29	38.2686535225851	40.0	38.0	41.0	34.0	41.0
30	38.215193062686055	40.0	38.0	41.0	34.0	41.0
31	38.121233875490745	40.0	38.0	41.0	34.0	41.0
32	38.069914146425646	40.0	37.0	41.0	34.0	41.0
33	37.991016868717374	40.0	37.0	41.0	33.0	41.0
34	37.95379265714656	40.0	37.0	41.0	33.0	41.0
35	37.86906251348203	40.0	37.0	41.0	33.0	41.0
36	37.815978256180166	40.0	37.0	41.0	33.0	41.0
37	37.76669744164977	40.0	37.0	41.0	33.0	41.0
38	37.73097027481772	40.0	37.0	41.0	33.0	41.0
39	37.664262478968034	40.0	37.0	41.0	33.0	41.0
40	37.366607705250445	39.0	36.0	41.0	32.0	41.0
41	37.42159454678804	39.0	36.0	41.0	33.0	41.0
42	37.37872557055956	39.0	36.0	41.0	33.0	41.0
43	36.84544458345916	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	1.0
13	2.0
14	1.0
15	2.0
16	5.0
17	4.0
18	15.0
19	40.0
20	83.0
21	182.0
22	343.0
23	616.0
24	1048.0
25	1766.0
26	2658.0
27	4045.0
28	6031.0
29	8818.0
30	12144.0
31	16621.0
32	22027.0
33	29903.0
34	43269.0
35	63233.0
36	100368.0
37	183486.0
38	359658.0
39	302576.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.29302385780232	17.47581862893136	13.440010354199922	26.791147159066398
2	19.530264463523018	20.541610940937918	34.32115276759136	25.60697182794771
3	20.296561542775788	21.047672462142454	30.46395444152034	28.191811553561415
4	14.9569869278226	16.663617930022863	34.680098364899266	33.69929677725527
5	15.794986841537598	34.24168428318737	34.06359204452306	15.899736830751973
6	32.927736313041976	35.13154148151344	16.09318779930109	15.847534406143494
7	29.500754993744337	29.305405755209456	20.688468009836487	20.505371241209716
8	26.75810000431425	32.576815220673886	20.533413865999396	20.131670909012467
9	28.012511324906164	13.715690927132318	18.385435092109237	39.88636265585228
10	18.40303723197722	25.019543552353422	30.77587471418094	25.801544501488415
11	37.55986021830105	20.585443720609174	20.091807239311446	21.762888821778333
12	22.191811553561415	24.793217999050864	27.89335174079986	25.12161870658786
13	33.72242115708184	18.425730186807023	22.538159540963804	25.31368911514733
14	23.217826480866304	20.84274558867941	24.84757754864317	31.09185038181112
15	28.121834419086245	25.56883385823375	21.4709866689676	24.838345053712413
16	24.331075542516935	25.396867854523492	23.435782389231633	26.836274213727947
17	25.411191164416064	25.000388282497088	23.279865395401007	26.308555157685838
18	25.642780102679147	22.651796885111523	24.899866258251002	26.805556753958328
19	27.057163812071273	23.434401829241985	24.848785538634107	24.659648820052634
20	28.35765132231762	22.06359204452306	24.559213080805904	25.019543552353422
21	26.26558522800811	23.693947107295397	23.46313473402649	26.577332930670007
22	26.746969239397732	24.05643038957677	23.024289227317833	26.172311143707667
23	26.34263773243022	23.52500107856249	24.144009663919928	25.98835152508736
24	26.39578929203158	23.05026101212304	24.54143837093921	26.012511324906164
25	26.324172742568702	23.283575650373184	24.931015143017387	25.461236464040727
26	26.375943742180425	23.803615341472888	24.118900729108244	25.701540187238447
27	25.762716251779626	23.194788386039086	25.28961560032788	25.752879761853404
28	25.16778118124164	24.196988653522585	23.95780663531645	26.677423529919324
29	25.808361016437292	24.555761680831786	23.98455498511584	25.651322317615083
30	25.439492644203803	24.019068984856982	24.87838129341214	25.663057077527075
31	26.495707321282197	23.552871133353467	24.29302385780232	25.65839768756202
32	24.181629923637775	23.844083006169377	24.528754476034344	27.445532594158507
33	24.60554812545839	23.138961991457784	26.299495232753785	25.955994650330037
34	24.856464903576516	23.067172871996203	25.586608568100438	26.489753656326847
35	24.655765995081754	24.53263730100522	25.879804995901463	24.93179170801156
36	24.83040683377195	23.71758919711808	26.108977954182667	25.343026014927304
37	25.467448983994135	23.293929850295527	25.36546011475905	25.873161050951293
38	24.512705466154706	22.862332283532506	26.423918201820612	26.201044048492168
39	24.20760170844299	21.803097631476767	27.31688166012339	26.67241899995686
40	23.826308296302688	22.74515725441132	27.91802925061478	25.51050519867121
41	22.76042969929678	22.111739074161957	28.753354329349847	26.374476897191425
42	21.856421761076835	22.29034902282238	29.33189525001079	26.521333966089994
43	21.05561068208292	22.75275033435437	29.267181500496136	26.924457483066565
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	28.0
2	29.0
3	68.5
4	108.0
5	108.0
6	171.5
7	235.0
8	269.0
9	303.0
10	475.5
11	648.0
12	648.0
13	1278.5
14	1909.0
15	3910.0
16	5911.0
17	5751.0
18	5591.0
19	5591.0
20	6057.5
21	6524.0
22	5854.0
23	5184.0
24	5766.0
25	6348.0
26	6348.0
27	7386.0
28	8424.0
29	11307.5
30	14191.0
31	15886.0
32	17581.0
33	17581.0
34	20601.0
35	23621.0
36	25865.0
37	28109.0
38	33187.0
39	38265.0
40	38265.0
41	44461.0
42	50657.0
43	55314.5
44	59972.0
45	76713.5
46	93455.0
47	93455.0
48	101077.0
49	108699.0
50	117385.0
51	126071.0
52	126512.5
53	126954.0
54	126954.0
55	113312.0
56	99670.0
57	93004.5
58	86339.0
59	77764.0
60	69189.0
61	69189.0
62	62205.5
63	55222.0
64	46163.5
65	37105.0
66	32198.5
67	27292.0
68	27292.0
69	23332.0
70	19372.0
71	16664.5
72	13957.0
73	11256.5
74	8556.0
75	8556.0
76	6921.5
77	5287.0
78	4308.0
79	3329.0
80	2688.0
81	2047.0
82	2047.0
83	1664.5
84	1282.0
85	1018.5
86	755.0
87	603.5
88	452.0
89	452.0
90	337.0
91	222.0
92	134.5
93	47.0
94	28.5
95	10.0
96	10.0
97	5.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1158950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.10164159782139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.0219069700016	42.729184871999735
2	6.517513776438741	6.40041251119117
3	2.0252985378376933	2.983364488004944
4	0.9952592265953167	1.9547544736483256
5	0.606319204468675	1.4885634135848531
6	0.43400630573510135	1.2786253245239667
7	0.30511813139030713	1.0487260792766688
8	0.23037535209217472	0.90494463771215
9	0.18616538354780102	0.8226923346796571
>10	1.3317968316291147	13.191603089454478
>50	0.1915800256665278	6.613898621411394
>100	0.13700497475335366	12.681320584817094
>500	0.012711801487390794	4.0988918198850905
>1k	0.004943478356207531	3.8030177498104787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3286	0.2835325078735062	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2599	0.22425471331808966	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2483	0.2142456533931576	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2115	0.1824927736313042	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2055	0.17731567367013243	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2025	0.17472712368954657	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1765	0.15229302385780233	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1727	0.14901419388239354	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1704	0.14702963889727771	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1623	0.14004055394969583	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	1511	0.13037663402217525	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1503	0.12968635402735235	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1486	0.1282195090383537	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1470	0.12683894904870788	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1318	0.11372362914707278	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	1310	0.11303334915224988	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1301	0.11225678415807412	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1295	0.11173907416195694	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1291	0.1113939341645455	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1236	0.10664825920013805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.628499935286251E-5	0.0
5	0.0	0.0	0.0	8.628499935286251E-5	0.0
6	0.0	0.0	0.0	8.628499935286251E-5	0.0
7	0.0	0.0	0.0	8.628499935286251E-5	0.0
8	8.628499935286251E-5	0.0	0.0	8.628499935286251E-5	0.0
9	8.628499935286251E-5	0.0	0.0	1.7256999870572502E-4	0.0
10	2.5885499805858753E-4	0.0	0.0	1.7256999870572502E-4	0.0
11	2.5885499805858753E-4	0.0	0.0	2.5885499805858753E-4	0.0
12	3.4513999741145004E-4	0.0	0.0	3.4513999741145004E-4	0.0
13	5.177099961171751E-4	0.0	0.0	4.3142499676431255E-4	0.0
14	5.177099961171751E-4	0.0	0.0	6.902799948229001E-4	0.0
15	6.039949954700375E-4	0.0	0.0	9.491349928814876E-4	0.0
16	6.039949954700375E-4	0.0	0.0	0.0018119849864101127	0.0
17	6.039949954700375E-4	0.0	0.0	0.0029336899779973252	0.0
18	6.039949954700375E-4	0.0	0.0	0.0036239699728202253	0.0
19	6.039949954700375E-4	0.0	0.0	0.004573104965701713	0.0
20	6.039949954700375E-4	0.0	0.0	0.005263384960524612	0.0
21	6.902799948229001E-4	0.0	0.0	0.008542214935933387	0.0
22	7.765649941757625E-4	0.0	0.0	0.0124250399068122	0.0
23	7.765649941757625E-4	0.0	0.0	0.015013589887398076	0.0
24	7.765649941757625E-4	0.0	0.0	0.0186375598602183	0.0
25	7.765649941757625E-4	0.0	0.0	0.02062211484533414	0.0
26	9.491349928814876E-4	0.0	0.0	0.023642089822684325	0.0
27	9.491349928814876E-4	0.0	0.0	0.03615341472884939	0.0
28	9.491349928814876E-4	0.0	0.0	0.10863281418525389	0.0
29	9.491349928814876E-4	0.0	0.0	0.23158893826308297	0.0
30	9.491349928814876E-4	0.0	0.0	0.39449501704128737	0.0
31	9.491349928814876E-4	0.0	0.0	0.9543983778420122	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1505	0.0	31.714283	1
ATACGGC	90	1.4370016E-10	24.666666	29
TTTTACG	80	2.7259375E-8	23.125002	4
AACGACC	155	0.0	20.290321	37
CTTATAC	1245	0.0	19.763052	37
TAGTTCC	160	0.0	19.65625	4
AACCGTC	50	0.0070349164	18.5	16
TATTAGA	70	1.2192086E-4	18.5	2
GCCTAGT	160	1.8189894E-12	18.5	1
TACAGCG	50	0.0070349164	18.5	5
AGTCGGT	290	0.0	18.5	11
CAGTCGG	300	0.0	18.499998	10
GTATCAA	2600	0.0	18.28654	2
GCAGTCG	305	0.0	18.196722	9
GTATTAG	265	0.0	18.150944	1
GCACCGT	155	2.0008883E-11	17.903225	6
TATACTC	85	2.7231474E-5	17.411764	5
CTAGTTC	160	3.45608E-11	17.34375	3
CTCTATG	470	0.0	17.319147	1
TACGGCT	130	1.3929821E-8	17.076923	30
>>END_MODULE