FastQCFastQC Report
Fri 10 Feb 2017
ERR1631675.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631675.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228117
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11560.5067574972492186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10760.47168777425619307No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9190.4028634428823805No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8680.3805064944743268No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8610.377437893712437No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8380.36735534835194217No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7310.3204495938487706No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA7200.3156275069372296No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6250.2739822108830118No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5820.2551322347742606No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5820.2551322347742606No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5640.24724154710082982No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5630.24680317556341702No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5260.23058342867914272No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5250.2301450571417299No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5140.22532297023018888No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5070.22225436946829916No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4940.2165555394819325No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4800.21041833795815307No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC4790.20997996642074024No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4770.2091032233459146No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4620.2025276502847223No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4490.19682882029835566No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4490.19682882029835566No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4460.1955137056861172No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4410.19332184799905314No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4400.19288347646164028No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4340.1902532472371634No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4320.18937650416233776No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4190.18367767417597108No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4160.18236255956373265No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4160.18236255956373265No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4140.181485816488907No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4140.181485816488907No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3860.16921141344134807No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3760.1648276980672199No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3700.162197468842743No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3650.16000561115567888No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3520.15430678116931223No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3510.15386840963189943No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3410.14948469425777122No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3400.14904632272035842No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3390.1486079511829456No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3370.14773120810811996No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3230.14159400658434051No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3190.13984052043468922No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3160.13852540582245076No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3140.1376486627476251No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3130.1372102912102123No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3080.1350184335231482No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA3070.13458006198573538No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3070.13458006198573538No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3070.13458006198573538No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA3000.13151146122384566No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC2960.12975797507419437No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2950.12931960353678157No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2850.12493588816265339No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2830.12405914508782773No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2830.12405914508782773No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2790.12230565893817646No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2730.11967542971369956No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2700.11836031510146108No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2690.11792194356404827No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG2680.11748357202663545No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2680.11748357202663545No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2680.11748357202663545No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2660.11660682895180982No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2660.11660682895180982No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2640.11573008587698416No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2600.1139765997273329No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2580.11309985665250727No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC2540.11134637050285599No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2490.10915451281579189No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC2480.10871614127837907No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA2480.10871614127837907No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2470.10827776974096626No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2460.10783939820355344No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA2450.10740102666614063No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2390.10477079744166372No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2380.10433242590425088No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.10433242590425088No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2350.10301731129201243No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2330.1021405682171868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATGC308.282786E-637.032
ATACGGC451.073513E-732.8888929
CAATGCT352.3799857E-531.71428533
GGTATCA6250.029.61
GGAAAAT400.001926470823.12536
GGGTCCA652.6672242E-622.7692319
TACGGCT652.6672242E-622.7692330
ATACACA903.787136E-922.6111137
CTCTATG1500.020.9666671
GCGGTAA3050.020.62295223
GAGCGTT450.003816554620.5555559
CCGGAAA450.003816554620.55555534
GAAAATC450.003816554620.55555537
CGCGGTA3150.020.55555522
GTATCAA9150.020.2185782
AATGCTG656.8711815E-519.92307734
CCGCGGT3250.019.92307721
CCCGTCC759.21249E-619.7333348
TCTATGG1600.019.656252
CTTATAC4900.019.63265437
Produced by FastQC (version 0.11.5)