FastQCFastQC Report
Fri 10 Feb 2017
ERR1631674.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631674.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147298
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA3920.2661271707694605No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3690.25051256636206876No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3620.24576029545547123No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3290.22335673261008296No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3280.22267783676628333No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA3090.2097788157340901No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2920.19823758638949615No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2870.1948431071704979No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2840.19280641963909897No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA2840.19280641963909897No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2600.17651291938790753No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2160.14664150226072317No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT1840.12491683525913454No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1830.1242379394153349No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1820.12355904357153527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.11880677266493775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1740.11812787682113811No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1740.11812787682113811No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1710.11609118928973916No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1690.11473339760213988No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1640.11133891838314167No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1630.11066002253934201No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1620.10998112669554237No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC1610.10930223085174272No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG1580.1072655433203438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGA200.0018367337.01
TAACCCT303.58439E-430.83333435
GACCGTT250.00548098529.67
AGGACCG250.00548098529.65
TATGCGG250.00548098529.65
GTTACGG250.00548098529.617
GTAGAAC250.00548098529.63
GGTATCA2300.028.1521741
CTTATTG601.3242225E-624.66666828
TTATTGA601.3242225E-624.66666829
TTCTGCG451.3155739E-424.66666618
GCGGTAA1250.023.68000223
TCCTCGC551.8880944E-523.54545421
CGCCTTA551.8880944E-523.54545425
TAGGACC400.00192324523.1254
AGAGCAA400.00192324523.12525
CAGTCGG652.656565E-622.7692310
TTAGGAC502.6866296E-422.23
CTGCGGG502.6866296E-422.220
AGCCGCG1350.021.92592419
Produced by FastQC (version 0.11.5)