FastQCFastQC Report
Fri 10 Feb 2017
ERR1631673.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631673.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5681
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA280.49287097342017255No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA150.2640380214750924No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT130.2288329519450801No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT120.2112304171800739No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA120.2112304171800739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT120.2112304171800739No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA120.2112304171800739No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA110.19362788241506776No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA110.19362788241506776No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC100.17602534765006161No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT100.17602534765006161No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG90.15842281288505544No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA90.15842281288505544No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA90.15842281288505544No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG90.15842281288505544No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG90.15842281288505544No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA90.15842281288505544No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG80.1408202781200493No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC80.1408202781200493No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC80.1408202781200493No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA80.1408202781200493No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA80.1408202781200493No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG70.12321774335504314No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC70.12321774335504314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70.12321774335504314No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA70.12321774335504314No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT60.10561520859003695No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC60.10561520859003695No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC60.10561520859003695No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT60.10561520859003695TruSeq Adapter, Index 1 (95% over 22bp)
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA60.10561520859003695No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG60.10561520859003695No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG60.10561520859003695No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT60.10561520859003695No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)