##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631673.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5681
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50888928005633	34.0	31.0	34.0	31.0	34.0
2	32.697060376694246	34.0	31.0	34.0	31.0	34.0
3	32.780496391480376	34.0	31.0	34.0	31.0	34.0
4	36.22900897729273	37.0	35.0	37.0	35.0	37.0
5	36.108607639500086	37.0	35.0	37.0	35.0	37.0
6	36.20383735257877	37.0	36.0	37.0	35.0	37.0
7	36.18623481781376	37.0	36.0	37.0	35.0	37.0
8	36.17426509417356	37.0	36.0	37.0	35.0	37.0
9	37.99348706213695	39.0	38.0	39.0	35.0	39.0
10	37.89596901953881	39.0	38.0	39.0	35.0	39.0
11	38.015842281288506	39.0	38.0	39.0	35.0	39.0
12	37.922372821686324	39.0	38.0	39.0	35.0	39.0
13	37.98468579475445	39.0	38.0	39.0	35.0	39.0
14	39.33726456609752	40.0	39.0	41.0	36.0	41.0
15	39.37070938215103	40.0	39.0	41.0	36.0	41.0
16	39.249955993663086	40.0	39.0	41.0	36.0	41.0
17	39.25030804435839	40.0	39.0	41.0	36.0	41.0
18	39.248899841577185	40.0	39.0	41.0	36.0	41.0
19	39.24819574018659	40.0	39.0	41.0	36.0	41.0
20	39.273015314205246	40.0	39.0	41.0	36.0	41.0
21	39.19397993311037	40.0	39.0	41.0	36.0	41.0
22	39.214222848090124	40.0	39.0	41.0	36.0	41.0
23	39.1286745291322	40.0	39.0	41.0	36.0	41.0
24	39.14152437951065	40.0	39.0	41.0	36.0	41.0
25	39.11177609575779	40.0	39.0	41.0	36.0	41.0
26	39.02323534588981	40.0	39.0	41.0	36.0	41.0
27	38.91762013729977	40.0	38.0	41.0	35.0	41.0
28	38.96866748811829	40.0	39.0	41.0	35.0	41.0
29	38.90230593205422	40.0	38.0	41.0	35.0	41.0
30	38.855131138884	40.0	38.0	41.0	35.0	41.0
31	38.73015314205246	40.0	38.0	41.0	35.0	41.0
32	38.663791585988385	40.0	38.0	41.0	35.0	41.0
33	38.62436190811477	40.0	38.0	41.0	35.0	41.0
34	38.55095933814469	40.0	38.0	41.0	35.0	41.0
35	38.54074986798099	40.0	38.0	41.0	35.0	41.0
36	38.437775039605704	40.0	38.0	41.0	34.0	41.0
37	38.44499207885936	40.0	38.0	41.0	34.0	41.0
38	38.34958634043302	40.0	38.0	41.0	34.0	41.0
39	38.282696708326	40.0	38.0	41.0	34.0	41.0
40	38.07058616440767	40.0	37.0	41.0	34.0	41.0
41	38.032740714662914	40.0	37.0	41.0	34.0	41.0
42	38.00228832951945	40.0	37.0	41.0	33.0	41.0
43	37.550607287449395	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	3.0
23	3.0
24	4.0
25	7.0
26	8.0
27	18.0
28	29.0
29	25.0
30	39.0
31	49.0
32	95.0
33	119.0
34	140.0
35	224.0
36	342.0
37	627.0
38	1407.0
39	2540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.770814997359615	17.215279000176025	12.849850378454498	28.16405562400986
2	20.876606231297306	19.4331983805668	31.596549903186062	28.093645484949832
3	21.263861996127442	19.978876958281994	28.780144340785075	29.97711670480549
4	15.807076218975533	15.067769758845273	35.36349234289738	33.761661679281815
5	16.51117760957578	34.14891744411195	33.40961098398169	15.930293962330575
6	34.219327583171975	33.48002112304172	15.490230593205423	16.810420700580885
7	30.892448512585812	28.69213166696004	18.817109663791587	21.59831015666256
8	28.37528604118993	29.7130786833304	21.052631578947366	20.859003696532298
9	29.589860939975352	13.219503608519625	17.338496743531067	39.85213870797395
10	19.30998063721176	23.37616616792818	29.57225840521035	27.74159478964971
11	38.97201196972364	19.133955289561698	18.51786657278648	23.37616616792818
12	22.689667312092944	24.99559936630875	26.03414891744411	26.2805844041542
13	34.870621369477206	17.602534765006162	20.190107375462066	27.33673649005457
14	23.957049815173384	19.785249075866925	22.988910403098046	33.26879070586165
15	30.417180073930645	23.65780672416828	19.503608519626823	26.421404682274247
16	25.31244499207886	24.5731385319486	22.302411547262807	27.812004928709733
17	27.03749339904946	23.305756028868156	21.756732969547617	27.900017602534767
18	26.527019890864285	20.859003696532298	23.411371237458194	29.20260517514522
19	28.005632811124805	21.65111776095758	23.02411547262806	27.31913395528956
20	29.501848266150326	21.158246787537404	23.094525611688084	26.245379334624186
21	26.879070586164406	22.51364196444288	21.351874669952473	29.25541277944024
22	29.273015314205246	22.40802675585284	20.63017074458722	27.68878718535469
23	28.14645308924485	23.164935750748107	21.51029748283753	27.178313677169513
24	27.600774511529657	21.73913043478261	22.32001408202781	28.34008097165992
25	27.882415067769763	21.59831015666256	22.848090124977997	27.671184650589687
26	28.2872733673649	22.777679985917974	22.74247491638796	26.192571730329167
27	26.931878190459425	22.584052103502906	23.552191515578244	26.931878190459425
28	26.298186938919205	23.23534588980813	22.09118113008273	28.37528604118993
29	27.05509593381447	23.728216863228305	22.42562929061785	26.791057912339376
30	25.664495687378984	22.355219151557822	23.728216863228305	28.25206829783489
31	27.723992254884706	22.42562929061785	22.883295194508012	26.967083259989437
32	25.594085548318958	22.038373525787712	22.40802675585284	29.959514170040485
33	26.33339200844922	21.756732969547617	24.872381622953704	27.03749339904946
34	27.125506072874494	20.929413835592324	24.273895440943498	27.671184650589687
35	25.54127794402394	24.90758669248372	24.8547790881887	24.696356275303643
36	25.224432318253832	22.038373525787712	25.400457665903893	27.33673649005457
37	26.703045238514346	22.636859707797925	25.154022179193802	25.506072874493928
38	27.195916211934517	21.035029044182362	25.25963738778384	26.50941735609928
39	25.928533708854072	20.31332511881711	26.31578947368421	27.442351698644607
40	25.558880478788947	21.721527900017605	26.104559056504133	26.61503256468932
41	23.059320542158073	21.36947720471748	28.551311388839995	27.019890864284456
42	22.496039429677875	21.246259461362435	28.762541806020064	27.495159302939626
43	21.351874669952473	21.73913043478261	29.185002640380215	27.723992254884706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	1.0
7	0.0
8	1.5
9	3.0
10	2.5
11	2.0
12	2.0
13	3.0
14	4.0
15	9.5
16	15.0
17	12.0
18	9.0
19	9.0
20	10.0
21	11.0
22	11.0
23	11.0
24	10.5
25	10.0
26	10.0
27	11.5
28	13.0
29	22.5
30	32.0
31	33.0
32	34.0
33	34.0
34	50.5
35	67.0
36	80.5
37	94.0
38	113.5
39	133.0
40	133.0
41	155.5
42	178.0
43	201.0
44	224.0
45	328.0
46	432.0
47	432.0
48	457.0
49	482.0
50	582.5
51	683.0
52	695.0
53	707.0
54	707.0
55	616.5
56	526.0
57	506.5
58	487.0
59	450.0
60	413.0
61	413.0
62	377.0
63	341.0
64	298.5
65	256.0
66	206.0
67	156.0
68	156.0
69	146.5
70	137.0
71	106.0
72	75.0
73	64.0
74	53.0
75	53.0
76	44.0
77	35.0
78	26.0
79	17.0
80	15.0
81	13.0
82	13.0
83	13.5
84	14.0
85	8.5
86	3.0
87	3.0
88	3.0
89	3.0
90	4.0
91	5.0
92	2.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5681.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.66819221967964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.22661122661123	77.23992254884703
2	5.25987525987526	8.906882591093117
3	1.7255717255717258	4.383031156486534
4	0.7900207900207901	2.6755852842809364
5	0.2910602910602911	1.2321774335504312
6	0.16632016632016633	0.8449216687202956
7	0.08316008316008316	0.49287097342017255
8	0.10395010395010396	0.7041013906002465
9	0.12474012474012475	0.9505368773103327
>10	0.2286902286902287	2.5699700756908994
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	28	0.49287097342017255	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	15	0.2640380214750924	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	13	0.2288329519450801	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	12	0.2112304171800739	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	12	0.2112304171800739	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.2112304171800739	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	12	0.2112304171800739	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	11	0.19362788241506776	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	11	0.19362788241506776	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	10	0.17602534765006161	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	10	0.17602534765006161	No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG	9	0.15842281288505544	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	9	0.15842281288505544	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	9	0.15842281288505544	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	9	0.15842281288505544	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	9	0.15842281288505544	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	9	0.15842281288505544	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	8	0.1408202781200493	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	8	0.1408202781200493	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	8	0.1408202781200493	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	8	0.1408202781200493	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	8	0.1408202781200493	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	7	0.12321774335504314	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	7	0.12321774335504314	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.12321774335504314	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	7	0.12321774335504314	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	6	0.10561520859003695	No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC	6	0.10561520859003695	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	6	0.10561520859003695	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	6	0.10561520859003695	TruSeq Adapter, Index 1 (95% over 22bp)
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	6	0.10561520859003695	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG	6	0.10561520859003695	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	6	0.10561520859003695	No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT	6	0.10561520859003695	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.08801267382503081	0.0
29	0.0	0.0	0.0	0.17602534765006161	0.0
30	0.0	0.0	0.0	0.33444816053511706	0.0
31	0.0	0.0	0.0	0.8625242034853019	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE