FastQCFastQC Report
Fri 10 Feb 2017
ERR1631672.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631672.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5208
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA200.38402457757296465No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG150.28801843317972353No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT130.24961597542242703RNA PCR Primer, Index 29 (95% over 23bp)
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT120.2304147465437788No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA110.21121351766513058No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA110.21121351766513058No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC100.19201228878648233No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT100.19201228878648233No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA100.19201228878648233No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA100.19201228878648233No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA100.19201228878648233No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC90.1728110599078341No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA90.1728110599078341No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT80.15360983102918588No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC80.15360983102918588No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG80.15360983102918588No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC80.15360983102918588No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC80.15360983102918588No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA70.13440860215053765No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG70.13440860215053765No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA70.13440860215053765No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG60.1152073732718894No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT60.1152073732718894No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA60.1152073732718894No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC60.1152073732718894No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA60.1152073732718894No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC60.1152073732718894No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC60.1152073732718894RNA PCR Primer, Index 29 (95% over 24bp)
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG60.1152073732718894No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGT200.00167857337.06
Produced by FastQC (version 0.11.5)