FastQCFastQC Report
Fri 10 Feb 2017
ERR1631671.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631671.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA80.5891016200294551No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA70.5154639175257731No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA60.4418262150220913No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC60.4418262150220913No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA50.3681885125184094No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA50.3681885125184094No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC50.3681885125184094No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA50.3681885125184094No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT50.3681885125184094No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA50.3681885125184094No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT50.3681885125184094No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC40.29455081001472755No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA40.29455081001472755No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA40.29455081001472755No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA30.22091310751104565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30.22091310751104565No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC30.22091310751104565No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT30.22091310751104565No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC30.22091310751104565No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTTAT30.22091310751104565No Hit
GTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATC30.22091310751104565No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC30.22091310751104565No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG30.22091310751104565No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT30.22091310751104565No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG30.22091310751104565No Hit
CTTCATGGTCACTAAAGATGACGGAGCCCGTACTCTGCGTTGA30.22091310751104565No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG30.22091310751104565No Hit
GACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCC30.22091310751104565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30.22091310751104565No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT30.22091310751104565TruSeq Adapter, Index 8 (95% over 22bp)
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG30.22091310751104565No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT30.22091310751104565No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG30.22091310751104565No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATAC30.22091310751104565No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC30.22091310751104565No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT30.22091310751104565No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA30.22091310751104565No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA30.22091310751104565No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG30.22091310751104565No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA20.14727540500736377No Hit
GATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGC20.14727540500736377No Hit
CAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAA20.14727540500736377No Hit
TTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACTATCATT20.14727540500736377No Hit
GGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGC20.14727540500736377No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCTGTCT20.14727540500736377No Hit
CAGGTGGTCAGGTAGAGAATACCAAGGCGCTTGAGAGAACTCG20.14727540500736377No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC20.14727540500736377No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20.14727540500736377No Hit
GTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTC20.14727540500736377No Hit
GGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACT20.14727540500736377No Hit
TCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG20.14727540500736377No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC20.14727540500736377No Hit
CGCTGTGAAGATGCAGTGTACCCGCGGCAAGACGGAAAGACCC20.14727540500736377No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT20.14727540500736377No Hit
CCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATT20.14727540500736377No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA20.14727540500736377No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG20.14727540500736377No Hit
TTAGTGGACCATGAAGTGCGATTGCCTCGGTGATAACGACATT20.14727540500736377No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT20.14727540500736377No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA20.14727540500736377No Hit
ATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGT20.14727540500736377No Hit
TAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCA20.14727540500736377No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC20.14727540500736377No Hit
AACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA20.14727540500736377No Hit
TTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTC20.14727540500736377No Hit
GCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGATGA20.14727540500736377No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA20.14727540500736377No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA20.14727540500736377No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTGTCT20.14727540500736377No Hit
GGCGTCCACACTTCAAAGCCTCCCACCTATCCTACACATCAAG20.14727540500736377No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT20.14727540500736377No Hit
CTAAAGATGACGGAGTGGCCCGTACTCTGCGTTGATACCTGTC20.14727540500736377No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGG20.14727540500736377No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20.14727540500736377No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA20.14727540500736377No Hit
GAGTACGGGCTCCGTCATCTTTAGTGACCATGAAGTGCGATTG20.14727540500736377No Hit
CTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTA20.14727540500736377No Hit
GTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGC20.14727540500736377No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT20.14727540500736377No Hit
GTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACAC20.14727540500736377No Hit
GAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAA20.14727540500736377No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA20.14727540500736377No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT20.14727540500736377No Hit
GCCTCGGTGATAACGACATTGGGGCCACTAAAGATGACGGAGT20.14727540500736377No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA20.14727540500736377No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG20.14727540500736377No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAC20.14727540500736377No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG20.14727540500736377No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC20.14727540500736377No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC20.14727540500736377No Hit
GCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCC20.14727540500736377No Hit
GCATCCTTGGCCACCTCGGCCACGCTGTCGGCGGCCAGCACAC20.14727540500736377No Hit
GTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTGT20.14727540500736377No Hit
GGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTA20.14727540500736377No Hit
TATCAACGCAGAGTACGGGCCGTCATCTTTAGTGACCATGAAG20.14727540500736377No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC20.14727540500736377No Hit
CCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAA20.14727540500736377No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT20.14727540500736377No Hit
ACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAA20.14727540500736377No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA20.14727540500736377No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT20.14727540500736377No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACCTGTCTCTTAT20.14727540500736377No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC20.14727540500736377No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC20.14727540500736377No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG20.14727540500736377No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA20.14727540500736377No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG20.14727540500736377No Hit
CATCAAATCGTACCCCAAACCGACACAGGTGGTCAGGTAGAGA20.14727540500736377No Hit
CACTAAAGATGACGGAGTGGACGCCCGTACTCTGCGTTGATAC20.14727540500736377No Hit
CAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT20.14727540500736377No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT20.14727540500736377No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC20.14727540500736377No Hit
GCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGA20.14727540500736377No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA20.14727540500736377No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT20.14727540500736377No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers