FastQCFastQC Report
Fri 10 Feb 2017
ERR1631670.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631670.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295943
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA9070.3064779366296888No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8090.27336345174577537No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7510.25376508314101026No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6790.22943607383854323No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6630.22402962732688392No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6320.21355463721054393No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5910.19970061802441685No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5780.19530788023369364No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5720.1932804627918214No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5560.18787401628016207No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5120.17300628837309887No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4350.1469877645357383No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3900.1317821337216964No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3890.13144423081471768No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3880.131106327907739No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3540.11961762907046289No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3370.11387327965182484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.11353537674484614No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3360.11353537674484614No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3350.11319747383786742No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3270.11049425058203775No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3240.10948054186110162No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3200.10812893023318679No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3200.10812893023318679No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3160.10677731860527197No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3150.10643941569829325No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC3050.10306038662850617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA200.001839728137.03
GATCGCA200.001839728137.020
AGATAAC303.5930786E-430.8333325
GGTATCA5200.030.2403851
TAGATAA250.005489832329.5999984
TTCGCTA250.005489832329.59999826
CGATCGC250.005489832329.59999819
GGGCCGA405.9254588E-527.75000215
GGCCGAT358.8537735E-426.42857216
TACGGCT451.3199703E-424.66666830
ATACGGC451.3199703E-424.66666829
GGTAATT400.001927817923.12500212
TAATTTG400.001927817923.12500214
TTGCGCG400.001927817923.12500218
TCAATGA1750.022.232
GGTCGGG502.6955374E-422.1999991
GCGGTAA2350.022.04255123
AATGAGC1450.021.68965734
CCGTGTC603.7142625E-521.5833329
AGCCGCG2500.021.46000119
Produced by FastQC (version 0.11.5)