##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631670.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	295943
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60352838215467	34.0	31.0	34.0	31.0	34.0
2	32.77206421506844	34.0	31.0	34.0	31.0	34.0
3	32.8494068114468	34.0	31.0	34.0	31.0	34.0
4	36.287203954815624	37.0	37.0	37.0	35.0	37.0
5	36.19818681300115	37.0	35.0	37.0	35.0	37.0
6	36.25140314182123	37.0	37.0	37.0	35.0	37.0
7	36.2484093220654	37.0	37.0	37.0	35.0	37.0
8	36.223225418408276	37.0	37.0	37.0	35.0	37.0
9	38.06059612830849	39.0	38.0	39.0	35.0	39.0
10	37.98468286122665	39.0	38.0	39.0	35.0	39.0
11	38.06534366415154	39.0	38.0	39.0	35.0	39.0
12	37.99113680674995	39.0	38.0	39.0	35.0	39.0
13	38.05547352023869	39.0	38.0	39.0	35.0	39.0
14	39.424345228642004	40.0	39.0	41.0	37.0	41.0
15	39.44228787300257	40.0	39.0	41.0	37.0	41.0
16	39.385202555897585	40.0	39.0	41.0	37.0	41.0
17	39.358116258874176	40.0	39.0	41.0	36.0	41.0
18	39.38601014384527	40.0	39.0	41.0	37.0	41.0
19	39.4060815765198	40.0	39.0	41.0	37.0	41.0
20	39.3983875273279	40.0	39.0	41.0	37.0	41.0
21	39.36592519505445	40.0	39.0	41.0	37.0	41.0
22	39.340967686345	40.0	39.0	41.0	36.0	41.0
23	39.27502255501904	40.0	39.0	41.0	36.0	41.0
24	39.284051320693514	40.0	39.0	41.0	36.0	41.0
25	39.23285565125717	40.0	39.0	41.0	36.0	41.0
26	39.18104161950105	40.0	39.0	41.0	36.0	41.0
27	39.06499900318642	40.0	39.0	41.0	36.0	41.0
28	39.05875117843639	40.0	39.0	41.0	35.0	41.0
29	39.03517907164555	40.0	39.0	41.0	35.0	41.0
30	39.00703851755237	40.0	39.0	41.0	35.0	41.0
31	38.912459493889024	40.0	38.0	41.0	35.0	41.0
32	38.87559090770858	40.0	38.0	41.0	35.0	41.0
33	38.81751891411522	40.0	38.0	41.0	35.0	41.0
34	38.80083326856861	40.0	38.0	41.0	35.0	41.0
35	38.729333013451914	40.0	38.0	41.0	35.0	41.0
36	38.69788438989941	40.0	38.0	41.0	35.0	41.0
37	38.65657575952126	40.0	38.0	41.0	35.0	41.0
38	38.62120070418966	40.0	38.0	41.0	35.0	41.0
39	38.55864135999162	40.0	38.0	41.0	35.0	41.0
40	38.25847207063522	40.0	38.0	41.0	34.0	41.0
41	38.337646100769405	40.0	38.0	41.0	34.0	41.0
42	38.336125537688	40.0	37.0	41.0	34.0	41.0
43	37.869978340423664	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	0.0
18	3.0
19	6.0
20	13.0
21	20.0
22	52.0
23	59.0
24	148.0
25	258.0
26	393.0
27	596.0
28	956.0
29	1370.0
30	1939.0
31	2685.0
32	3630.0
33	4933.0
34	7373.0
35	10952.0
36	17342.0
37	30661.0
38	71061.0
39	141491.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.850964543847965	16.65151735300379	13.358315621589293	28.13920248155895
2	20.55801286058464	19.701091088486635	32.6377038821665	27.10319216876223
3	20.54213142395664	19.918700560580923	29.683756669358623	29.85541134610381
4	15.396883859391842	16.282865281490018	34.45122878392123	33.869022075196916
5	16.44167964777001	33.0708954089132	33.99438405368602	16.493040889630773
6	33.27228554147251	34.14339923566362	16.035182450674622	16.54913277218924
7	29.59421239900927	27.98478085306968	20.548213676282256	21.872793071638796
8	27.054196247250317	30.829923329830404	20.846244040237476	21.2696363826818
9	28.61328026005008	12.72305815646932	17.962918534988155	40.70074304849244
10	19.11077470999483	23.705578439091312	29.632057524590884	27.551589326322972
11	38.84531818627235	19.627766157672255	18.732661357085657	22.794254298969737
12	22.310377336176224	24.46011563037477	26.524702392014675	26.704804641434333
13	35.18684341241388	17.856141216382884	20.859422253609647	26.097593117593593
14	23.67955991525395	20.788800546051096	23.503174597811068	32.028464940883886
15	29.547919700753184	24.435110815258344	20.18868498325691	25.828284500731556
16	25.01360059200589	24.511476872235534	22.81114944431867	27.663773091439907
17	26.75717959201603	24.04584666641887	21.795075403033692	27.401898338531407
18	26.433806510037407	21.473729738496942	23.53291005362519	28.55955369784046
19	27.855026136789856	22.404314344316305	23.571093082113787	26.169566436780055
20	30.063559536802696	20.82934889488854	23.171016040250993	25.936075528057767
21	27.180234031553375	22.508726342572725	22.090402543733084	28.22063708214082
22	28.549416610631102	22.981790412342917	21.40817657454307	27.06061640248291
23	27.887802718766793	22.290441064664478	22.540489215828725	27.281267000740005
24	27.393788668763918	21.804536684429095	23.458233511182897	27.34344113562409
25	27.60227476236978	22.18636696931504	23.281172387926052	26.93018588038913
26	27.30424439841456	23.331182018158902	22.472570731526005	26.892002851900536
27	26.469624218177152	22.038027593151384	24.745981489678755	26.746366698992713
28	25.805982908870966	23.11458625478555	22.45770300361894	28.62172783272455
29	27.126507469343757	23.691724419905185	22.703020514085484	26.478747596665574
30	26.50814514957272	22.119800096640233	24.14012157746593	27.231933176321117
31	27.689453712370288	22.75539546466718	23.171016040250993	26.384134782711538
32	25.379549440263833	22.661120553620123	23.003416198389555	28.955913807726485
33	25.274799539100435	22.169471823966102	25.244388277472353	27.311340359461113
34	26.150981776896227	21.851843091406113	24.43071807746762	27.566457054230035
35	25.26770357805388	23.779579175719647	25.08253278502955	25.87018446119692
36	25.630611300149013	22.498251352456386	25.35184140189158	26.519295945503018
37	26.14996806817529	22.36072486931605	24.984540942005722	26.504766120502936
38	25.4356413228223	21.55043369838111	25.98067871177896	27.033246267017635
39	24.94196517572641	20.258968787908483	27.209969487367502	27.58909654899761
40	24.445585805374684	21.435884612915324	28.00066228969768	26.117867292012313
41	22.733431775713566	20.321818728606523	29.25766110365847	27.687088392021437
42	21.077369628610914	20.934436698958923	30.203113437384904	27.78508023504526
43	20.25694137046661	21.697083560009865	30.05747728447708	27.988497785046444
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	6.5
4	11.0
5	11.0
6	14.5
7	18.0
8	24.0
9	30.0
10	46.5
11	63.0
12	63.0
13	137.0
14	211.0
15	417.0
16	623.0
17	594.0
18	565.0
19	565.0
20	639.5
21	714.0
22	640.5
23	567.0
24	672.5
25	778.0
26	778.0
27	881.5
28	985.0
29	1379.0
30	1773.0
31	2065.0
32	2357.0
33	2357.0
34	2906.0
35	3455.0
36	3993.5
37	4532.0
38	5739.0
39	6946.0
40	6946.0
41	8195.5
42	9445.0
43	11130.0
44	12815.0
45	18007.5
46	23200.0
47	23200.0
48	25941.5
49	28683.0
50	32450.5
51	36218.0
52	36547.5
53	36877.0
54	36877.0
55	33014.0
56	29151.0
57	27164.5
58	25178.0
59	23157.5
60	21137.0
61	21137.0
62	19076.5
63	17016.0
64	13991.0
65	10966.0
66	9240.0
67	7514.0
68	7514.0
69	6367.5
70	5221.0
71	4415.0
72	3609.0
73	2846.0
74	2083.0
75	2083.0
76	1682.0
77	1281.0
78	1055.5
79	830.0
80	645.0
81	460.0
82	460.0
83	378.5
84	297.0
85	220.5
86	144.0
87	124.5
88	105.0
89	105.0
90	81.0
91	57.0
92	38.0
93	19.0
94	12.5
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	295943.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.080995266718325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.92122696405362	46.13865237056279
2	5.72739401403878	6.080315490996466
3	2.099311174540295	3.343005795574268
4	1.1375848486905502	2.415365438753343
5	0.7203136776165573	1.9117483456058473
6	0.5143269241717316	1.6380591016503285
7	0.3753215473068298	1.394570889326386
8	0.3080815843487558	1.308262169646351
9	0.24368538339630025	1.1641556414364707
>10	1.6938755714449008	17.49075298399143
>50	0.1653768076212421	6.08070628544816
>100	0.08650479167880357	8.58659201840742
>500	0.0069967110916679355	2.4478134686007307
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	907	0.3064779366296888	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	809	0.27336345174577537	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	751	0.25376508314101026	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	679	0.22943607383854323	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	663	0.22402962732688392	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	632	0.21355463721054393	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	591	0.19970061802441685	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	578	0.19530788023369364	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	572	0.1932804627918214	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	556	0.18787401628016207	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	512	0.17300628837309887	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	435	0.1469877645357383	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	390	0.1317821337216964	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	389	0.13144423081471768	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	388	0.131106327907739	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	354	0.11961762907046289	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	337	0.11387327965182484	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	336	0.11353537674484614	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	336	0.11353537674484614	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	335	0.11319747383786742	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	327	0.11049425058203775	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	324	0.10948054186110162	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	320	0.10812893023318679	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	320	0.10812893023318679	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	316	0.10677731860527197	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	315	0.10643941569829325	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	305	0.10306038662850617	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.379029069787087E-4	0.0
4	0.0	0.0	0.0	3.379029069787087E-4	0.0
5	0.0	0.0	0.0	3.379029069787087E-4	0.0
6	0.0	0.0	0.0	3.379029069787087E-4	0.0
7	0.0	0.0	0.0	3.379029069787087E-4	0.0
8	6.758058139574174E-4	0.0	0.0	3.379029069787087E-4	0.0
9	6.758058139574174E-4	0.0	0.0	3.379029069787087E-4	0.0
10	6.758058139574174E-4	0.0	0.0	3.379029069787087E-4	0.0
11	6.758058139574174E-4	0.0	0.0	3.379029069787087E-4	0.0
12	6.758058139574174E-4	0.0	0.0	3.379029069787087E-4	0.0
13	0.0013516116279148349	0.0	0.0	3.379029069787087E-4	0.0
14	0.0016895145348935438	0.0	0.0	3.379029069787087E-4	0.0
15	0.0016895145348935438	0.0	0.0	3.379029069787087E-4	0.0
16	0.0016895145348935438	0.0	0.0	0.0016895145348935438	0.0
17	0.0016895145348935438	0.0	0.0	0.0016895145348935438	0.0
18	0.0016895145348935438	0.0	0.0	0.0016895145348935438	0.0
19	0.0016895145348935438	0.0	0.0	0.0020274174418722524	0.0
20	0.0016895145348935438	0.0	0.0	0.0023653203488509613	0.0
21	0.0016895145348935438	0.0	0.0	0.004054834883744505	0.0
22	0.0016895145348935438	0.0	0.0	0.007771766860510301	0.0
23	0.0016895145348935438	0.0	0.0	0.008785475581446427	0.0
24	0.0016895145348935438	0.0	0.0	0.012502407558212223	0.0
25	0.0016895145348935438	0.0	0.0	0.014191922093105767	0.0
26	0.0016895145348935438	0.0	0.0	0.019598368604765107	0.0
27	0.0016895145348935438	0.0	0.0	0.04088625174442376	0.0
28	0.0016895145348935438	0.0	0.0	0.1432708325589725	0.0
29	0.0016895145348935438	0.0	0.0	0.3355375866298578	0.0
30	0.0016895145348935438	0.0	0.0	0.5703801069800604	0.0
31	0.0016895145348935438	0.0	0.0	1.1806327569836084	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATA	20	0.0018397281	37.0	3
GATCGCA	20	0.0018397281	37.0	20
AGATAAC	30	3.5930786E-4	30.833332	5
GGTATCA	520	0.0	30.240385	1
TAGATAA	25	0.0054898323	29.599998	4
TTCGCTA	25	0.0054898323	29.599998	26
CGATCGC	25	0.0054898323	29.599998	19
GGGCCGA	40	5.9254588E-5	27.750002	15
GGCCGAT	35	8.8537735E-4	26.428572	16
TACGGCT	45	1.3199703E-4	24.666668	30
ATACGGC	45	1.3199703E-4	24.666668	29
GGTAATT	40	0.0019278179	23.125002	12
TAATTTG	40	0.0019278179	23.125002	14
TTGCGCG	40	0.0019278179	23.125002	18
TCAATGA	175	0.0	22.2	32
GGTCGGG	50	2.6955374E-4	22.199999	1
GCGGTAA	235	0.0	22.042551	23
AATGAGC	145	0.0	21.689657	34
CCGTGTC	60	3.7142625E-5	21.583332	9
AGCCGCG	250	0.0	21.460001	19
>>END_MODULE