FastQCFastQC Report
Fri 10 Feb 2017
ERR1631669.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631669.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416601
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25810.18219668064613817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21750.1535365286343861No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20900.1475362505038469No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20620.14555968829613986No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA16180.11421705900249964No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA15800.11153458172061152No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA15650.11047570910933989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.028.4328861
TAGACTA502.7025325E-422.1999995
TTTTACG1202.382876E-1020.0416664
CTTATAC14750.019.69152537
TATTAGA1355.638867E-1119.1851862
CCGTTTA1002.876095E-718.49999827
TCTTATA28250.018.07433537
AGGACCG1950.018.0256425
GGACCGT1850.018.06
GTATTAG2900.017.8620681
TTAGGAC3250.017.6461543
CTAAGTC1603.45608E-1117.3437534
GTATCAA24450.017.2515342
GCGGTAA7900.017.09493623
GTTACGG1301.3938916E-817.07692317
TAGGACC1851.8189894E-1217.04
CGCGGTA7950.016.75471722
AACGACC1554.0199666E-1016.70967937
TATAATC1005.8797323E-616.653
GACCGTT1452.9813236E-916.5862067
Produced by FastQC (version 0.11.5)