##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631669.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1416601
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62904304034799	34.0	31.0	34.0	31.0	34.0
2	32.79675857916237	34.0	31.0	34.0	31.0	34.0
3	32.889604059294044	34.0	31.0	34.0	31.0	34.0
4	36.30357595399128	37.0	37.0	37.0	35.0	37.0
5	36.22102342155625	37.0	35.0	37.0	35.0	37.0
6	36.270844789746725	37.0	37.0	37.0	35.0	37.0
7	36.260765028402496	37.0	37.0	37.0	35.0	37.0
8	36.236407428767876	37.0	37.0	37.0	35.0	37.0
9	38.06671109225533	39.0	38.0	39.0	37.0	39.0
10	38.005834388088104	39.0	38.0	39.0	35.0	39.0
11	38.079201553577896	39.0	38.0	39.0	37.0	39.0
12	38.02017858239547	39.0	38.0	39.0	35.0	39.0
13	38.06590987864614	39.0	38.0	39.0	36.0	39.0
14	39.47485424618506	41.0	39.0	41.0	37.0	41.0
15	39.473072516537826	41.0	39.0	41.0	37.0	41.0
16	39.43622516149572	40.0	39.0	41.0	37.0	41.0
17	39.404651697972824	40.0	39.0	41.0	37.0	41.0
18	39.41968698313781	41.0	39.0	41.0	37.0	41.0
19	39.4515421067753	41.0	39.0	41.0	37.0	41.0
20	39.43657105988207	41.0	39.0	41.0	37.0	41.0
21	39.40438909756523	40.0	39.0	41.0	37.0	41.0
22	39.382460551700866	40.0	39.0	41.0	36.0	41.0
23	39.313502531764414	40.0	39.0	41.0	36.0	41.0
24	39.33232363947223	41.0	39.0	41.0	36.0	41.0
25	39.277127433906934	40.0	39.0	41.0	36.0	41.0
26	39.21898826839738	40.0	39.0	41.0	36.0	41.0
27	39.11649504694688	40.0	39.0	41.0	36.0	41.0
28	39.10493286394687	40.0	39.0	41.0	35.0	41.0
29	39.08336645251556	40.0	39.0	41.0	35.0	41.0
30	39.036428041488044	40.0	39.0	41.0	35.0	41.0
31	38.95217425372423	40.0	38.0	41.0	35.0	41.0
32	38.89835599438374	40.0	38.0	41.0	35.0	41.0
33	38.8472293892211	40.0	38.0	41.0	35.0	41.0
34	38.80515614488483	40.0	38.0	41.0	35.0	41.0
35	38.738166216175195	40.0	38.0	41.0	35.0	41.0
36	38.68458796795993	40.0	38.0	41.0	35.0	41.0
37	38.647990506854086	40.0	38.0	41.0	35.0	41.0
38	38.60984709173578	40.0	38.0	41.0	35.0	41.0
39	38.54439111648234	40.0	38.0	41.0	35.0	41.0
40	38.25411036699819	40.0	38.0	41.0	34.0	41.0
41	38.30815522507749	40.0	38.0	41.0	34.0	41.0
42	38.28929740978582	40.0	37.0	41.0	34.0	41.0
43	37.82962033769565	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	3.0
17	6.0
18	18.0
19	28.0
20	77.0
21	99.0
22	223.0
23	401.0
24	673.0
25	1213.0
26	1879.0
27	3018.0
28	4463.0
29	6581.0
30	9121.0
31	12670.0
32	17424.0
33	23692.0
34	34532.0
35	52151.0
36	81246.0
37	146506.0
38	326677.0
39	693898.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.05743113269015	16.79251955914192	12.723625071562141	27.42642423660579
2	19.795129327171164	19.679853395557394	34.07388530715424	26.451131970117203
3	20.36339096188694	20.394451225150906	29.31573534114405	29.926422471818103
4	15.183597922068387	15.831486777151788	33.646171363707914	35.33874393707191
5	16.54417863604501	33.66113676328056	33.29462565676574	16.500058943908694
6	34.54091872023245	33.91166602310742	15.531755236654499	16.015660020005633
7	30.608548207999288	28.484449749788403	20.075448203128474	20.831553839083835
8	27.185142464250696	32.2419650981469	19.98946774709322	20.583424690509183
9	27.633116170326012	13.82019354779504	17.98749259671566	40.55919768516329
10	18.738586235644334	24.4821230537039	30.723259407553716	26.05603130309805
11	37.87926169754222	20.491655730865645	19.79195270933735	21.83712986225479
12	22.773455616648583	23.89614295062618	27.58320797458141	25.747193458143826
13	33.45084466268201	18.125640176732897	22.45275839844812	25.970756762136975
14	23.85209384999728	19.789623189592554	23.98692362916587	32.37135933124429
15	27.975626164318673	25.016147807321893	20.954241878976507	26.053984149382924
16	25.491863975812525	24.524548549662185	22.744018958055232	27.239568516470058
17	25.85519846449353	24.344610797253424	23.15352029258768	26.646670445665364
18	26.006405473383122	22.727500545319394	24.270066165419905	26.996027815877582
19	27.53697053722255	23.037679628914564	24.11377656799621	25.311573265866677
20	28.243309160448142	22.22757148978435	23.84771717653736	25.68140217323015
21	26.740557150531448	23.131142784736138	23.08554067094404	27.04275939378837
22	27.03224125918307	23.681403585060295	22.551233551296377	26.735121604460254
23	26.752487115285106	23.313410056889698	23.410967520141522	26.523135307683678
24	26.68824884353463	22.78729155210253	23.674626800348157	26.849832804014678
25	27.041065197610337	23.044738779656374	23.859435366768768	26.054760655964525
26	26.792371316976336	23.394943247957613	23.585469726479086	26.227215708586964
27	26.592950308520187	22.877578090090296	24.1156825386965	26.41378906269302
28	26.024547490789573	23.782208257653355	23.401437666640078	26.791806584916994
29	26.287924404966535	24.111376456743994	23.336281705293164	26.264417432996307
30	25.962285781246802	23.94400399265566	24.20829859642906	25.885411629668482
31	26.738086447771813	23.265690197875056	23.57481040885895	26.421412945494176
32	24.64300109910977	23.451910594444026	24.234135088144086	27.67095321830212
33	25.23568739539221	22.916262236155415	25.20582718775435	26.64222318069802
34	25.461015487070814	22.68726338609107	25.081727317713316	26.7699938091248
35	25.394094738038447	23.563798133701727	25.308749605569954	25.733357522689875
36	25.202650569920536	22.948875512582585	25.66806037832812	26.180413539168757
37	25.579962177070325	22.256443416318355	25.396283074768405	26.76731133184291
38	24.63749496153116	21.912168634640242	26.51014647031874	26.940189933509863
39	24.223334587509115	21.23441957191898	27.460590526196153	27.081655314375748
40	23.909908294572713	21.80352830472377	28.155493325220014	26.1310700754835
41	22.898049627241544	21.27995109420366	29.023486500433076	26.798512778121715
42	22.191146271956605	21.455229807122826	29.413504578918126	26.94011934200244
43	21.292869340061173	21.830635443572323	29.466377617974292	27.41011759839221
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	25.0
2	25.0
3	73.0
4	121.0
5	121.0
6	168.5
7	216.0
8	225.0
9	234.0
10	386.5
11	539.0
12	539.0
13	1095.5
14	1652.0
15	3219.0
16	4786.0
17	4758.0
18	4730.0
19	4730.0
20	5310.5
21	5891.0
22	5554.5
23	5218.0
24	6069.0
25	6920.0
26	6920.0
27	8049.5
28	9179.0
29	12040.5
30	14902.0
31	17898.5
32	20895.0
33	20895.0
34	24854.0
35	28813.0
36	32253.5
37	35694.0
38	41705.5
39	47717.0
40	47717.0
41	54565.5
42	61414.0
43	66867.5
44	72321.0
45	87918.5
46	103516.0
47	103516.0
48	111964.5
49	120413.0
50	129937.5
51	139462.0
52	141884.0
53	144306.0
54	144306.0
55	133695.0
56	123084.0
57	115808.0
58	108532.0
59	99362.5
60	90193.0
61	90193.0
62	83045.5
63	75898.0
64	65557.5
65	55217.0
66	48815.5
67	42414.0
68	42414.0
69	36858.5
70	31303.0
71	27079.0
72	22855.0
73	18935.0
74	15015.0
75	15015.0
76	12285.0
77	9555.0
78	7850.5
79	6146.0
80	4817.5
81	3489.0
82	3489.0
83	2723.5
84	1958.0
85	1537.5
86	1117.0
87	828.5
88	540.0
89	540.0
90	393.0
91	246.0
92	145.0
93	44.0
94	23.5
95	3.0
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1416601.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.919880116684254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.42204636390434	48.88630352224744
2	6.727959898590736	7.524534219181063
3	1.9562910232269335	3.281866784765871
4	0.9555937913909813	2.137467610193258
5	0.5836427206485422	1.631861548482096
6	0.37653481506582226	1.2633469030943196
7	0.2692519380412627	1.0539575267516614
8	0.21785065794686473	0.974574614058357
9	0.15476305108247532	0.778889813672187
>10	1.0935797832270686	12.143970425447376
>50	0.13560026320997373	5.341383773754664
>100	0.09724952602414697	10.373654583129438
>500	0.007607500769045649	2.927746176046348
>1k	0.0020286668717455062	1.680442499175998
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2581	0.18219668064613817	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2175	0.1535365286343861	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2090	0.1475362505038469	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2062	0.14555968829613986	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1618	0.11421705900249964	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	1580	0.11153458172061152	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1565	0.11047570910933989	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	7.059150741810856E-5	0.0	0.0	0.0	0.0
6	7.059150741810856E-5	0.0	0.0	0.0	0.0
7	7.059150741810856E-5	0.0	0.0	0.0	0.0
8	1.4118301483621713E-4	0.0	0.0	0.0	0.0
9	1.4118301483621713E-4	0.0	0.0	0.0	0.0
10	1.4118301483621713E-4	0.0	0.0	7.059150741810856E-5	0.0
11	1.4118301483621713E-4	0.0	0.0	1.4118301483621713E-4	0.0
12	1.4118301483621713E-4	0.0	0.0	1.4118301483621713E-4	0.0
13	1.4118301483621713E-4	0.0	0.0	2.1177452225432566E-4	0.0
14	1.4118301483621713E-4	0.0	0.0	2.1177452225432566E-4	0.0
15	1.4118301483621713E-4	0.0	0.0	2.8236602967243426E-4	0.0
16	1.4118301483621713E-4	7.059150741810856E-5	0.0	7.059150741810855E-4	0.0
17	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.0010588726112716283	0.0
18	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.0016236046706164969	0.0
19	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.0020471537151251482	0.0
20	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.002894251804142451	0.0
21	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.0052237715489400335	0.0
22	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.00783565732341005	0.0
23	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.008965121442099787	0.0
24	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.011224049679479261	7.059150741810856E-5
25	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.012353513798168998	7.059150741810856E-5
26	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.014824216557802796	7.059150741810856E-5
27	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.034025106575528326	7.059150741810856E-5
28	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.12762944541194027	7.059150741810856E-5
29	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.28490732393948615	7.059150741810856E-5
30	1.4118301483621713E-4	7.059150741810856E-5	0.0	0.48312827676953496	7.059150741810856E-5
31	1.4118301483621713E-4	7.059150741810856E-5	0.0	1.0993921365296226	7.059150741810856E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1490	0.0	28.432886	1
TAGACTA	50	2.7025325E-4	22.199999	5
TTTTACG	120	2.382876E-10	20.041666	4
CTTATAC	1475	0.0	19.691525	37
TATTAGA	135	5.638867E-11	19.185186	2
CCGTTTA	100	2.876095E-7	18.499998	27
TCTTATA	2825	0.0	18.074335	37
AGGACCG	195	0.0	18.025642	5
GGACCGT	185	0.0	18.0	6
GTATTAG	290	0.0	17.862068	1
TTAGGAC	325	0.0	17.646154	3
CTAAGTC	160	3.45608E-11	17.34375	34
GTATCAA	2445	0.0	17.251534	2
GCGGTAA	790	0.0	17.094936	23
GTTACGG	130	1.3938916E-8	17.076923	17
TAGGACC	185	1.8189894E-12	17.0	4
CGCGGTA	795	0.0	16.754717	22
AACGACC	155	4.0199666E-10	16.709679	37
TATAATC	100	5.8797323E-6	16.65	3
GACCGTT	145	2.9813236E-9	16.586206	7
>>END_MODULE