FastQCFastQC Report
Fri 10 Feb 2017
ERR1631668.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631668.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18982
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA880.46359709198187754No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA690.3635022653039722No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC620.32662522389632287No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA570.30028448003371616No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA560.2950163312611948No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT550.28974818248867346No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG480.2528711410810241No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC460.24233484353598148No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA460.24233484353598148No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT400.21072595090085344No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA360.1896533558107681No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA360.1896533558107681No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA330.17384890949320408No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA310.16331261194816143No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC310.16331261194816143No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT300.15804446317564008No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA290.15277631440311876No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT280.1475081656305974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT280.1475081656305974No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA280.1475081656305974No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG260.13697186808555473No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA260.13697186808555473No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA250.1317037193130334No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT240.12643557054051205No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA240.12643557054051205No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT230.12116742176799074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT230.12116742176799074No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG230.12116742176799074No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC230.12116742176799074No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG230.12116742176799074No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG230.12116742176799074No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA230.12116742176799074No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA220.11589927299546939No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA220.11589927299546939No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC220.11589927299546939No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA210.11063112422294806No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC210.11063112422294806No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT200.10536297545042672No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG200.10536297545042672No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA200.10536297545042672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT190.1000948266779054No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC190.1000948266779054No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC190.1000948266779054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA303.468783E-430.8333321
GCCTTGG250.005362729.61
CCTTGGA250.005362729.62
TGGAGGA250.005362729.65
AACTCCG250.005362729.65
CTTGGAG250.005362729.63
TAACTCC250.005362729.64
CTAACTC250.005362729.63
GCTAACT250.005362729.62
GGCTAAC250.005362729.61
GGAGGAT250.005362729.66
TCTTATA358.550339E-426.4285737
GTATCAA450.00369074120.5555572
CTCTTAT701.1409355E-418.537
GACCATG803.1684246E-416.18759
GAAGTGC855.0269195E-415.23529415
GATAACG855.0269195E-415.23529434
ATGAAGT855.0269195E-415.23529413
TGAAGTG855.0269195E-415.23529414
AAGTGCG855.0269195E-415.23529416
Produced by FastQC (version 0.11.5)