##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631668.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18982
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.546148983247285	34.0	31.0	34.0	31.0	34.0
2	32.70740701717416	34.0	31.0	34.0	31.0	34.0
3	32.80028448003372	34.0	31.0	34.0	31.0	34.0
4	36.254398904225056	37.0	37.0	37.0	35.0	37.0
5	36.16447160467811	37.0	35.0	37.0	35.0	37.0
6	36.21135812875356	37.0	36.0	37.0	35.0	37.0
7	36.20508903171426	37.0	36.0	37.0	35.0	37.0
8	36.15814982615109	37.0	36.0	37.0	35.0	37.0
9	37.984669687071964	39.0	38.0	39.0	35.0	39.0
10	37.91702665683279	39.0	38.0	39.0	35.0	39.0
11	38.01180065325045	39.0	38.0	39.0	35.0	39.0
12	37.917764197660944	39.0	38.0	39.0	35.0	39.0
13	37.961963965862395	39.0	38.0	39.0	35.0	39.0
14	39.314403118744075	40.0	39.0	41.0	36.0	41.0
15	39.34738173006006	40.0	39.0	41.0	36.0	41.0
16	39.30455168053946	40.0	39.0	41.0	36.0	41.0
17	39.29859867242651	40.0	39.0	41.0	36.0	41.0
18	39.31429775576862	40.0	39.0	41.0	36.0	41.0
19	39.330418291012535	40.0	39.0	41.0	36.0	41.0
20	39.322094615951954	40.0	39.0	41.0	36.0	41.0
21	39.29238225687494	40.0	39.0	41.0	36.0	41.0
22	39.23495943525445	40.0	39.0	41.0	36.0	41.0
23	39.18301548835739	40.0	39.0	41.0	36.0	41.0
24	39.21899694447371	40.0	39.0	41.0	36.0	41.0
25	39.15915077441787	40.0	39.0	41.0	36.0	41.0
26	39.09503740385629	40.0	39.0	41.0	36.0	41.0
27	38.99088610262354	40.0	39.0	41.0	35.0	41.0
28	39.01201137920135	40.0	39.0	41.0	35.0	41.0
29	38.964492677273206	40.0	38.0	41.0	35.0	41.0
30	38.944684437888526	40.0	38.0	41.0	35.0	41.0
31	38.86603097671478	40.0	38.0	41.0	35.0	41.0
32	38.804815087978085	40.0	38.0	41.0	35.0	41.0
33	38.74533768833632	40.0	38.0	41.0	35.0	41.0
34	38.72194710778632	40.0	38.0	41.0	35.0	41.0
35	38.640343483299965	40.0	38.0	41.0	35.0	41.0
36	38.571172689916764	40.0	38.0	41.0	35.0	41.0
37	38.52618269939943	40.0	38.0	41.0	35.0	41.0
38	38.4694974186071	40.0	38.0	41.0	35.0	41.0
39	38.39015909809293	40.0	38.0	41.0	34.0	41.0
40	38.12627752607734	40.0	37.0	41.0	34.0	41.0
41	38.1786429248762	40.0	37.0	41.0	34.0	41.0
42	38.18902117795807	40.0	37.0	41.0	34.0	41.0
43	37.68675587398588	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	3.0
23	2.0
24	8.0
25	11.0
26	26.0
27	44.0
28	61.0
29	100.0
30	140.0
31	170.0
32	259.0
33	380.0
34	510.0
35	772.0
36	1167.0
37	1952.0
38	4820.0
39	8556.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.35591613107154	15.941418185649564	13.344220840796545	28.35844484248235
2	21.09893583394795	19.31303340006322	31.566747444947847	28.021283321040986
3	20.087451269623855	19.381519334105995	29.64914129175008	30.881888104520073
4	15.915077441786957	16.357601938678748	34.35886629438416	33.36845432515014
5	16.589400484669685	32.84690759667053	34.06384996312296	16.499841955536823
6	33.473817300600565	34.2218944262986	15.583184069118111	16.72110420398272
7	30.1970287640923	27.55241808028659	20.292909071752185	21.957644083868928
8	27.415446212201033	30.439363607628277	20.82499209777684	21.320198082393844
9	28.490148561795387	12.601411863871038	17.890633231482457	41.017806342851124
10	19.571172689916764	23.21673164050153	29.49636497734696	27.715730692234747
11	39.21609946264882	18.93372668844168	18.075018438520704	23.77515541038879
12	22.568749341481404	24.00695395637973	26.651564640185438	26.77273206195343
13	35.56527236329154	17.51659466863344	20.624802444421032	26.293330523653992
14	24.022758402697292	20.208618691391845	23.316826467179432	32.45179643873143
15	30.386682119903064	23.327362764724477	20.335054261932356	25.9509008534401
16	25.30291855441998	24.201875460963016	22.089347803181962	28.405858181435047
17	26.61995574755031	24.244020651143188	20.867137287957014	28.26888631334949
18	26.64629649141292	21.151617321673164	22.890106416605207	29.311979770308717
19	27.905384048045516	21.994521125276577	23.611842798440627	26.488252028237273
20	30.68696659993678	20.15593720366663	22.71098935833948	26.446106838057105
21	27.325887683068167	22.147297439679697	21.694236645242864	28.832578232009272
22	29.865135391423454	22.500263407438624	20.46675798124539	27.16784321989253
23	28.242545569486882	21.820672215783375	21.994521125276577	27.94226108945317
24	28.02655146981351	21.53619218206722	22.642503424296702	27.794752923822568
25	27.868507006637866	21.868085554736066	23.248340533136655	27.01506690548941
26	27.76314403118744	23.432725740174902	22.27900115899273	26.525129069644926
27	26.38815720155937	21.509851438204613	25.034242967021385	27.067748393214625
28	26.224844589611212	22.79527973869982	21.55199662838479	29.427879043304184
29	27.115161732167316	23.606574649668108	22.431777473395847	26.84648614476873
30	26.519860920872407	21.64682330629017	23.696133178800967	28.137182594036453
31	28.35317669370983	22.768938994837214	22.4686545148035	26.40922979664946
32	26.01411863871036	21.968180381413973	22.4686545148035	29.549046465072177
33	25.187019281424504	22.04193446422927	25.30291855441998	27.46812769992625
34	26.82014540090612	21.393952165209146	24.038562849014856	27.747339584869877
35	25.619007480771256	23.517016120535246	25.139605942471814	25.724370456221685
36	26.30386682119903	22.373827836898112	24.897271098935832	26.425034242967023
37	25.90348751448741	23.30102202086187	24.33357918027605	26.461911284374672
38	25.73490675376673	21.910230744916237	25.798124539036984	26.556737962280053
39	25.0869244547466	21.267516594668635	25.78758824149194	27.85797070909283
40	25.076388157201556	21.76272257928564	27.00979875671689	26.15109050679591
41	23.011273838373196	20.840796544094406	27.894847750500475	28.253081867031927
42	21.2833210409862	20.89347803181962	28.9379412074597	28.885259719734485
43	20.761774312506585	22.142029290907175	28.98535454641239	28.110841850173852
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	1.5
9	1.0
10	2.5
11	4.0
12	4.0
13	6.5
14	9.0
15	23.0
16	37.0
17	46.5
18	56.0
19	56.0
20	48.0
21	40.0
22	34.5
23	29.0
24	27.5
25	26.0
26	26.0
27	35.5
28	45.0
29	49.5
30	54.0
31	70.0
32	86.0
33	86.0
34	139.5
35	193.0
36	206.0
37	219.0
38	307.0
39	395.0
40	395.0
41	463.0
42	531.0
43	631.0
44	731.0
45	1119.5
46	1508.0
47	1508.0
48	1649.5
49	1791.0
50	2074.5
51	2358.0
52	2403.5
53	2449.0
54	2449.0
55	2187.0
56	1925.0
57	1790.0
58	1655.0
59	1519.5
60	1384.0
61	1384.0
62	1269.5
63	1155.0
64	956.5
65	758.0
66	653.5
67	549.0
68	549.0
69	450.0
70	351.0
71	304.0
72	257.0
73	215.5
74	174.0
75	174.0
76	129.5
77	85.0
78	72.5
79	60.0
80	45.0
81	30.0
82	30.0
83	23.0
84	16.0
85	12.0
86	8.0
87	8.0
88	8.0
89	8.0
90	4.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	18982.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.41070487830576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.27209894507094	63.91844905700137
2	5.98035649327028	8.660836582025077
3	2.124408875954893	4.61489832472869
4	1.1058566751546017	3.2030344536929727
5	0.6620589305201892	2.397007691497208
6	0.3710440160058203	1.6120535243915288
7	0.327391778828665	1.6594668633442209
8	0.19643506729719898	1.1379201348646086
9	0.18915969443433978	1.2327468127699925
>10	0.7275372862859221	9.524812980718576
>50	0.04365223717715533	2.0387735749657567
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	88	0.46359709198187754	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	69	0.3635022653039722	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	62	0.32662522389632287	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	57	0.30028448003371616	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	56	0.2950163312611948	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	55	0.28974818248867346	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	48	0.2528711410810241	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	46	0.24233484353598148	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	46	0.24233484353598148	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	40	0.21072595090085344	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	36	0.1896533558107681	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	36	0.1896533558107681	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	33	0.17384890949320408	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	31	0.16331261194816143	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	31	0.16331261194816143	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	30	0.15804446317564008	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	29	0.15277631440311876	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	28	0.1475081656305974	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28	0.1475081656305974	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	28	0.1475081656305974	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	26	0.13697186808555473	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	26	0.13697186808555473	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	25	0.1317037193130334	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	24	0.12643557054051205	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	24	0.12643557054051205	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	23	0.12116742176799074	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23	0.12116742176799074	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	23	0.12116742176799074	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	23	0.12116742176799074	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG	23	0.12116742176799074	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	23	0.12116742176799074	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	23	0.12116742176799074	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	22	0.11589927299546939	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	22	0.11589927299546939	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	22	0.11589927299546939	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	21	0.11063112422294806	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	21	0.11063112422294806	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	20	0.10536297545042672	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG	20	0.10536297545042672	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	20	0.10536297545042672	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.1000948266779054	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	19	0.1000948266779054	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	19	0.1000948266779054	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.005268148772521336	0.0	0.0	0.0	0.0
20	0.005268148772521336	0.0	0.0	0.0	0.0
21	0.005268148772521336	0.0	0.0	0.0	0.0
22	0.005268148772521336	0.0	0.0	0.0	0.0
23	0.005268148772521336	0.0	0.0	0.0	0.0
24	0.005268148772521336	0.0	0.0	0.0	0.0
25	0.005268148772521336	0.0	0.0	0.0	0.0
26	0.005268148772521336	0.0	0.0	0.0	0.0
27	0.005268148772521336	0.0	0.0	0.010536297545042672	0.0
28	0.005268148772521336	0.0	0.0	0.09482667790538404	0.0
29	0.005268148772521336	0.0	0.0	0.27394373617110945	0.0
30	0.005268148772521336	0.0	0.0	0.5057422821620483	0.0
31	0.005268148772521336	0.0	0.0	1.0062164155515751	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30	3.468783E-4	30.833332	1
GCCTTGG	25	0.0053627	29.6	1
CCTTGGA	25	0.0053627	29.6	2
TGGAGGA	25	0.0053627	29.6	5
AACTCCG	25	0.0053627	29.6	5
CTTGGAG	25	0.0053627	29.6	3
TAACTCC	25	0.0053627	29.6	4
CTAACTC	25	0.0053627	29.6	3
GCTAACT	25	0.0053627	29.6	2
GGCTAAC	25	0.0053627	29.6	1
GGAGGAT	25	0.0053627	29.6	6
TCTTATA	35	8.550339E-4	26.42857	37
GTATCAA	45	0.003690741	20.555557	2
CTCTTAT	70	1.1409355E-4	18.5	37
GACCATG	80	3.1684246E-4	16.1875	9
GAAGTGC	85	5.0269195E-4	15.235294	15
GATAACG	85	5.0269195E-4	15.235294	34
ATGAAGT	85	5.0269195E-4	15.235294	13
TGAAGTG	85	5.0269195E-4	15.235294	14
AAGTGCG	85	5.0269195E-4	15.235294	16
>>END_MODULE