FastQCFastQC Report
Fri 10 Feb 2017
ERR1631667.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631667.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198377
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6610.33320395005469383No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5420.273217157230929No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4720.2379308085110673No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4660.23490626433507916No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4420.2228080876311266No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4210.2122221830151681No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3760.189538101695257No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3490.17592765290331036No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3440.17340719942332025No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3410.17189492733532616No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3300.1663499296793479No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3200.16130902271936767No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3000.15122720879940718No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2920.14719448323142298No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2620.13207176235148227No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2490.12551858330350799No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2420.12198994843152179No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2390.12047767634352773No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2370.1194694949515317No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2250.11342040659955538No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2150.10837949963957516No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2130.1073713182475791No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2130.1073713182475791No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2120.10686722755158108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.10636313685558306No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2100.10585904615958504No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2080.10485086476758898No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2000.10081813919960479No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2000.10081813919960479No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1990.10031404850360677No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1990.10031404850360677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCC303.5888434E-430.8333349
ATACGGC250.00548552129.629
CTAGGCG250.00548552129.62
GGCTCGT250.00548552129.621
GGTATCA3450.027.8840581
AACCGAC358.8434364E-426.4285727
ACTTTTG358.8434364E-426.42857230
CTCGTCA400.001925589423.12519
CATCTCA400.001925589423.1256
ACGCTTC652.6643083E-622.7692325
TATACAC502.6911948E-422.237
AATGATA603.7063146E-521.5833344
ACTAACA705.0668896E-621.14285933
ATAGGAG450.00381482320.5555573
CTATCAT450.00381482320.5555571
AACTTTT450.00381482320.55555729
GTAACGT1650.020.18181826
TTCTGCG1750.020.08571418
TCAATGA1403.6379788E-1219.8214332
GTATCAA4800.019.656252
Produced by FastQC (version 0.11.5)