##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631667.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	198377
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60358307666716	34.0	31.0	34.0	31.0	34.0
2	32.7802870292423	34.0	31.0	34.0	31.0	34.0
3	32.86755521053348	34.0	31.0	34.0	31.0	34.0
4	36.27902428204882	37.0	37.0	37.0	35.0	37.0
5	36.20160603295745	37.0	35.0	37.0	35.0	37.0
6	36.25484809226876	37.0	37.0	37.0	35.0	37.0
7	36.25290230218221	37.0	37.0	37.0	35.0	37.0
8	36.22744068112735	37.0	37.0	37.0	35.0	37.0
9	38.082685996864555	39.0	38.0	39.0	37.0	39.0
10	37.98989298154524	39.0	38.0	39.0	35.0	39.0
11	38.07796266704305	39.0	38.0	39.0	37.0	39.0
12	37.997832410007206	39.0	38.0	39.0	35.0	39.0
13	38.06904026172389	39.0	38.0	39.0	37.0	39.0
14	39.44817695599792	40.0	39.0	41.0	37.0	41.0
15	39.463415617737944	41.0	39.0	41.0	37.0	41.0
16	39.403554847588175	40.0	39.0	41.0	37.0	41.0
17	39.38098670712834	40.0	39.0	41.0	36.0	41.0
18	39.406680209903364	40.0	39.0	41.0	37.0	41.0
19	39.417003987357404	41.0	39.0	41.0	37.0	41.0
20	39.419514359023474	41.0	39.0	41.0	37.0	41.0
21	39.362794073909775	40.0	39.0	41.0	36.0	41.0
22	39.34361846383401	40.0	39.0	41.0	36.0	41.0
23	39.277965691587234	40.0	39.0	41.0	36.0	41.0
24	39.2903007909183	40.0	39.0	41.0	36.0	41.0
25	39.23589428209924	40.0	39.0	41.0	36.0	41.0
26	39.1783976973137	40.0	39.0	41.0	36.0	41.0
27	39.0464922848919	40.0	39.0	41.0	35.0	41.0
28	39.03565433492794	40.0	39.0	41.0	35.0	41.0
29	39.00279266245583	40.0	39.0	41.0	35.0	41.0
30	38.978697127187125	40.0	38.0	41.0	35.0	41.0
31	38.88815235637196	40.0	38.0	41.0	35.0	41.0
32	38.84887865024675	40.0	38.0	41.0	35.0	41.0
33	38.78717794905659	40.0	38.0	41.0	35.0	41.0
34	38.761590305327736	40.0	38.0	41.0	35.0	41.0
35	38.69362879769328	40.0	38.0	41.0	35.0	41.0
36	38.64049763833509	40.0	38.0	41.0	35.0	41.0
37	38.596021716227185	40.0	38.0	41.0	35.0	41.0
38	38.55561380603598	40.0	38.0	41.0	35.0	41.0
39	38.47163733698967	40.0	38.0	41.0	34.0	41.0
40	38.184250190294236	40.0	37.0	41.0	34.0	41.0
41	38.248446140429586	40.0	37.0	41.0	34.0	41.0
42	38.232723551621405	40.0	37.0	41.0	34.0	41.0
43	37.75658468471647	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	0.0
16	0.0
17	1.0
18	1.0
19	5.0
20	8.0
21	11.0
22	22.0
23	60.0
24	114.0
25	161.0
26	272.0
27	392.0
28	593.0
29	945.0
30	1391.0
31	1770.0
32	2480.0
33	3329.0
34	4995.0
35	7588.0
36	11719.0
37	20935.0
38	47819.0
39	93764.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.04934543823124	16.042182309441113	12.562444234966755	28.346028017360887
2	21.26204146650065	19.55115764428336	31.925071958946855	27.261728930269136
3	20.713590789254802	19.56174354889932	29.58659522021202	30.138070441633857
4	15.359139416363792	15.626307485242744	34.7706639378557	34.24388916053777
5	16.43083623605559	33.52303946526059	33.659143953180056	16.386980345503762
6	33.5976448882683	34.68345624744804	15.6121929457548	16.106705918528863
7	30.091190006906043	27.91906319785056	20.034076531049465	21.955670264193934
8	27.744143726339242	30.44455758480066	20.37534593224013	21.435952756619972
9	28.574885193343984	12.364336591439532	17.295351779692204	41.765426435524276
10	18.93314245098978	23.635804553955346	29.576009315596064	27.855043679458806
11	39.95422856480338	19.11763964572506	18.611028496247044	22.317103293224516
12	22.9078975889342	23.954893964522096	26.57364513023183	26.56356331631187
13	35.95880570832304	17.301904958740177	20.601178564047242	26.13811076888954
14	23.96447168774606	19.756322557554558	23.261265166828814	33.01794058787057
15	29.673298819923684	24.046134380497737	19.818325713162313	26.462241086416267
16	25.358282462180597	24.17366932658524	22.120507921785286	28.34754028944888
17	27.37565342756469	23.506253245083855	21.309425991924467	27.80866733542699
18	26.958266331278324	20.978238404653766	23.17556974850915	28.88792551555876
19	28.384338910256734	22.198137888968983	22.98956028168588	26.427962919088404
20	30.649722498071853	20.37282547876014	22.290386486336622	26.68706553683139
21	27.5762815245719	21.938027089834	21.644646304763153	28.84104508083094
22	28.794164646103127	22.43405233469606	20.79827802618247	27.973504993018345
23	28.416600714800605	21.863925757522292	22.057496584785536	27.661976942891563
24	28.01484043009018	21.233812387524765	22.49252685543183	28.258820326953227
25	28.166571729585588	21.9647438967219	22.774313554494725	27.09437081919779
26	28.43676434264053	22.33424237688845	22.080684756801443	27.148308523669577
27	26.853919557206734	21.578106332891416	24.126788891857423	27.441185218044428
28	26.49500698165614	22.96183529340599	21.93399436426602	28.60916336067185
29	27.224930309461275	23.819293567298626	22.029771596505643	26.92600452673445
30	26.957258149886325	21.88459347605821	23.55111731702768	27.607031057027783
31	28.0859172182259	22.356926458208363	22.702732675663007	26.85442364790273
32	25.740383209747097	21.99498933848178	22.91092213311019	29.353705318660932
33	26.11593077826563	21.8104921437465	24.49427100923998	27.57930606874789
34	26.64573009975955	21.970288894377877	23.57833821461157	27.805642791251
35	25.718203219123186	23.876255815946404	24.169636601017256	26.235904363913154
36	26.354869768168687	22.51269048327175	24.681792748151246	26.45064700040831
37	27.039424933334004	22.31861556531251	23.85457991601849	26.787379585334993
38	26.05291944126587	21.7343744486508	25.068430311981732	27.144275798101596
39	25.531185570907915	20.705525338118836	25.835656351290726	27.927632739682522
40	25.253431597413005	21.547356800435537	26.970868598678273	26.228343003473185
41	23.616145016811423	20.815417109846404	27.65744012662758	27.91099774671459
42	22.27879240032867	21.138539245981136	28.959002303694483	27.623666049995716
43	21.407219586948084	21.678420381395018	28.604122453711874	28.310237577945024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	4.5
4	8.0
5	8.0
6	9.5
7	11.0
8	11.0
9	11.0
10	20.0
11	29.0
12	29.0
13	63.5
14	98.0
15	214.0
16	330.0
17	323.5
18	317.0
19	317.0
20	359.0
21	401.0
22	350.0
23	299.0
24	348.5
25	398.0
26	398.0
27	477.5
28	557.0
29	801.5
30	1046.0
31	1217.0
32	1388.0
33	1388.0
34	1767.0
35	2146.0
36	2476.0
37	2806.0
38	3610.5
39	4415.0
40	4415.0
41	5394.5
42	6374.0
43	7285.0
44	8196.0
45	11430.5
46	14665.0
47	14665.0
48	16159.5
49	17654.0
50	20029.5
51	22405.0
52	23062.0
53	23719.0
54	23719.0
55	21306.5
56	18894.0
57	17969.0
58	17044.0
59	16263.5
60	15483.0
61	15483.0
62	14081.5
63	12680.0
64	10469.5
65	8259.0
66	7249.5
67	6240.0
68	6240.0
69	5288.0
70	4336.0
71	3766.5
72	3197.0
73	2554.5
74	1912.0
75	1912.0
76	1555.0
77	1198.0
78	994.5
79	791.0
80	628.5
81	466.0
82	466.0
83	374.0
84	282.0
85	214.5
86	147.0
87	123.5
88	100.0
89	100.0
90	75.5
91	51.0
92	33.5
93	16.0
94	11.5
95	7.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	198377.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.283083392758094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.43163127156342	46.37492364693637
2	6.73820036431213	7.315405845861367
3	2.3841945208005004	3.8826428999151146
4	1.2397539943181584	2.6919067784031014
5	0.8099208466795965	2.198250043092089
6	0.5702141753131252	1.8571790178153098
7	0.4094331388728739	1.5557705254836443
8	0.3045780397184227	1.322674810371034
9	0.2506527163191065	1.2245582072314254
>10	1.6277446992850668	16.711234909052596
>50	0.14879384055479777	5.562280438821906
>100	0.08288515950367929	8.651050923219842
>500	0.001997232759124802	0.6521219537961945
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	661	0.33320395005469383	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	542	0.273217157230929	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	472	0.2379308085110673	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	466	0.23490626433507916	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	442	0.2228080876311266	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	421	0.2122221830151681	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	376	0.189538101695257	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	349	0.17592765290331036	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	344	0.17340719942332025	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	341	0.17189492733532616	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	330	0.1663499296793479	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	320	0.16130902271936767	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	300	0.15122720879940718	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	292	0.14719448323142298	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	262	0.13207176235148227	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	249	0.12551858330350799	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	242	0.12198994843152179	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	239	0.12047767634352773	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	237	0.1194694949515317	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	225	0.11342040659955538	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	215	0.10837949963957516	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	213	0.1073713182475791	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	213	0.1073713182475791	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	212	0.10686722755158108	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	211	0.10636313685558306	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	210	0.10585904615958504	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	208	0.10485086476758898	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	200	0.10081813919960479	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	200	0.10081813919960479	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	199	0.10031404850360677	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	199	0.10031404850360677	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	5.04090695998024E-4	0.0	0.0	0.0
10	0.0	5.04090695998024E-4	0.0	0.0	0.0
11	0.0	5.04090695998024E-4	0.0	0.0	0.0
12	0.0	5.04090695998024E-4	0.0	0.0	0.0
13	0.0	5.04090695998024E-4	0.0	0.0	0.0
14	0.0	5.04090695998024E-4	0.0	0.0	0.0
15	0.0	5.04090695998024E-4	0.0	5.04090695998024E-4	0.0
16	0.0	5.04090695998024E-4	0.0	5.04090695998024E-4	0.0
17	0.0	5.04090695998024E-4	0.0	0.00252045347999012	0.0
18	5.04090695998024E-4	5.04090695998024E-4	0.0	0.00252045347999012	0.0
19	5.04090695998024E-4	5.04090695998024E-4	0.0	0.00252045347999012	0.0
20	5.04090695998024E-4	5.04090695998024E-4	0.0	0.003024544175988144	0.0
21	5.04090695998024E-4	5.04090695998024E-4	0.0	0.00907363252796443	0.0
22	5.04090695998024E-4	5.04090695998024E-4	0.0	0.011089995311956527	0.0
23	5.04090695998024E-4	5.04090695998024E-4	0.0	0.012098176703952575	0.0
24	5.04090695998024E-4	5.04090695998024E-4	0.0	0.017139083663932814	0.0
25	5.04090695998024E-4	5.04090695998024E-4	0.0	0.01764317435993084	0.0
26	5.04090695998024E-4	5.04090695998024E-4	0.0	0.02268408131991108	0.0
27	5.04090695998024E-4	5.04090695998024E-4	0.0	0.03528634871986168	0.0
28	5.04090695998024E-4	5.04090695998024E-4	0.0	0.11039586242356725	0.0
29	5.04090695998024E-4	5.04090695998024E-4	0.0	0.2510371666070159	0.0
30	5.04090695998024E-4	5.04090695998024E-4	0.0	0.42847709159832037	0.0
31	5.04090695998024E-4	5.04090695998024E-4	0.0	0.8504010041486664	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATCC	30	3.5888434E-4	30.833334	9
ATACGGC	25	0.005485521	29.6	29
CTAGGCG	25	0.005485521	29.6	2
GGCTCGT	25	0.005485521	29.6	21
GGTATCA	345	0.0	27.884058	1
AACCGAC	35	8.8434364E-4	26.428572	7
ACTTTTG	35	8.8434364E-4	26.428572	30
CTCGTCA	40	0.0019255894	23.125	19
CATCTCA	40	0.0019255894	23.125	6
ACGCTTC	65	2.6643083E-6	22.76923	25
TATACAC	50	2.6911948E-4	22.2	37
AATGATA	60	3.7063146E-5	21.583334	4
ACTAACA	70	5.0668896E-6	21.142859	33
ATAGGAG	45	0.003814823	20.555557	3
CTATCAT	45	0.003814823	20.555557	1
AACTTTT	45	0.003814823	20.555557	29
GTAACGT	165	0.0	20.181818	26
TTCTGCG	175	0.0	20.085714	18
TCAATGA	140	3.6379788E-12	19.82143	32
GTATCAA	480	0.0	19.65625	2
>>END_MODULE