FastQCFastQC Report
Fri 10 Feb 2017
ERR1631666.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631666.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13544
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA270.1993502658003544No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA250.1845835794447726No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA210.15505020673360898No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA210.15505020673360898No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC200.14766686355581807No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT200.14766686355581807RNA PCR Primer, Index 33 (95% over 22bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA200.14766686355581807No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC200.14766686355581807No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT190.1402835203780272No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA180.13290017720023625No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC170.12551683402244537No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC170.12551683402244537No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA170.12551683402244537No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT170.12551683402244537No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT170.12551683402244537No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG170.12551683402244537No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT170.12551683402244537No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC160.11813349084465447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT160.11813349084465447No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC160.11813349084465447No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA160.11813349084465447No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT150.11075014766686357No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC150.11075014766686357No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA150.11075014766686357No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT140.10336680448907265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA251.1702141E-437.01
GTATCAA303.4161482E-430.8333322
ATCAACG400.001834629923.1254
TCAACGC450.003636296420.5555555
AGAGTAC450.003636296420.55555512
GCAGAGT450.003636296420.55555510
CGCAGAG450.003636296420.5555559
AACGCAG500.00668965518.57
ACGCAGA500.00668965518.58
CAGAGTA500.00668965518.511
AGTACGG500.00668965518.514
GAGTACG500.00668965518.513
TATCAAC500.00668965518.53
CAACGCA500.00668965518.56
Produced by FastQC (version 0.11.5)