FastQCFastQC Report
Fri 10 Feb 2017
ERR1631665.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631665.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207368
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8570.41327495081208293No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8160.3935033370626133No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7240.3491377647467305No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6710.32357933721692833No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5900.28451834419968364No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5830.281142702827823No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5740.27680259249257355No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5590.2695690752671579No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5450.26281779252343657No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5430.26185332356004787No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4780.23050808224991318No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4020.19385826164114037No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3980.1919293237143629No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3700.17842675822692028No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3570.17215770996489332No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3450.16637089618456077No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3250.1567262065506732No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3180.15335056517881254No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3130.15093939277034063No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3000.1446703445083137No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2980.14370587554492495No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2920.1408124686547587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.1408124686547587No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2880.13888353072798118No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2880.13888353072798118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2870.13840129624628678No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2850.13743682728289802No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2790.13454342039273176No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2790.13454342039273176No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2740.13213224798425985No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC2720.1311677790208711No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC2700.13020331005748237No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2650.12779213764901046No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2630.1268276686856217No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2580.1244164962771498No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2570.12393426179545543No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2500.12055862042359476No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2470.11911191697851163No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2450.11814744801512288No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2440.1176652135334285No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2440.1176652135334285No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2430.11718297905173411No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2400.11573627560665098No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG2350.11332510319817908No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2340.1128428687164847No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2310.11139616527140157No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2280.10994946182631843No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2240.10802052389954091No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2230.10753828941784654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2230.10753828941784654No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2210.10657382045445778No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT2200.10609158597276339No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG2190.10560935149106902No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2170.10464488252768026No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA2150.1036804135642915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA251.2301387E-437.017
CAGTATT251.2301387E-437.014
ATTGACT308.279147E-637.031
TATTGAC352.3789471E-531.71428730
AGTATTA303.5894016E-430.83333215
TAAACCA250.005486087429.65
CGTAAAA250.005486087429.632
GCCGTCC250.005486087429.625
CTCGTAA358.844798E-426.42857230
ATTAGAG358.844798E-426.42857218
CTACGGG358.844798E-426.42857211
AACGACC509.044254E-625.90000237
CTCTATG1300.025.6153851
GGTATCA4650.025.0645181
GGGCAGT1150.024.1304367
AAACACG701.9071558E-723.78571515
TCTATGG1400.023.7857152
GCAGTCG1103.6379788E-1223.5454549
CAGTCGG1103.6379788E-1223.54545410
CCTACGG400.001925882823.12510
Produced by FastQC (version 0.11.5)