##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631665.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	207368
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48439489217237	33.0	31.0	34.0	31.0	34.0
2	32.65617163689672	34.0	31.0	34.0	31.0	34.0
3	32.73175224721268	34.0	31.0	34.0	31.0	34.0
4	36.19935091238764	37.0	35.0	37.0	35.0	37.0
5	36.10737915203889	37.0	35.0	37.0	35.0	37.0
6	36.167904401836346	37.0	36.0	37.0	35.0	37.0
7	36.16291327495081	37.0	36.0	37.0	35.0	37.0
8	36.13934647583041	37.0	36.0	37.0	35.0	37.0
9	37.97257050268122	39.0	38.0	39.0	35.0	39.0
10	37.88952008024382	39.0	38.0	39.0	35.0	39.0
11	37.96940704448131	39.0	38.0	39.0	35.0	39.0
12	37.88467362370279	39.0	38.0	39.0	35.0	39.0
13	37.94358338798657	39.0	38.0	39.0	35.0	39.0
14	39.26814648354616	40.0	39.0	41.0	36.0	41.0
15	39.28209752710158	40.0	39.0	41.0	36.0	41.0
16	39.21092936229312	40.0	39.0	41.0	36.0	41.0
17	39.18675494772578	40.0	39.0	41.0	36.0	41.0
18	39.23390301300104	40.0	39.0	41.0	36.0	41.0
19	39.246725627869296	40.0	39.0	41.0	36.0	41.0
20	39.24397206897882	40.0	39.0	41.0	36.0	41.0
21	39.192908259712205	40.0	39.0	41.0	36.0	41.0
22	39.14375892133791	40.0	39.0	41.0	36.0	41.0
23	39.08271768064504	40.0	39.0	41.0	36.0	41.0
24	39.09510628447977	40.0	39.0	41.0	36.0	41.0
25	39.02971046641719	40.0	39.0	41.0	35.0	41.0
26	38.963403225184216	40.0	38.0	41.0	35.0	41.0
27	38.82839203734424	40.0	38.0	41.0	35.0	41.0
28	38.821578064117894	40.0	38.0	41.0	35.0	41.0
29	38.7839251957872	40.0	38.0	41.0	35.0	41.0
30	38.73494946182632	40.0	38.0	41.0	35.0	41.0
31	38.63662667335365	40.0	38.0	41.0	35.0	41.0
32	38.60084005246711	40.0	38.0	41.0	35.0	41.0
33	38.51979572547356	40.0	38.0	41.0	35.0	41.0
34	38.50492843640291	40.0	38.0	41.0	34.0	41.0
35	38.40411249565989	40.0	38.0	41.0	34.0	41.0
36	38.378853053508735	40.0	38.0	41.0	34.0	41.0
37	38.33011361444389	40.0	38.0	41.0	34.0	41.0
38	38.27886173372941	40.0	38.0	41.0	34.0	41.0
39	38.204433663824695	40.0	37.0	41.0	34.0	41.0
40	37.894207399405886	40.0	37.0	41.0	33.0	41.0
41	37.969286485860884	40.0	37.0	41.0	33.0	41.0
42	37.9363884495197	40.0	37.0	41.0	33.0	41.0
43	37.433726515180744	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	1.0
17	2.0
18	4.0
19	5.0
20	7.0
21	17.0
22	36.0
23	82.0
24	117.0
25	225.0
26	341.0
27	539.0
28	798.0
29	1208.0
30	1745.0
31	2245.0
32	3087.0
33	4089.0
34	6023.0
35	8849.0
36	13610.0
37	23468.0
38	53337.0
39	87531.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.58275143705876	16.535338142818564	13.138960688245053	27.74294973187763
2	21.59928243509124	19.57871995679179	31.84290729524324	26.979090312873733
3	20.990702519192933	19.83430423208981	30.164249064465103	29.01074418425215
4	15.154700821727557	16.42490644651055	35.854133713977085	32.56625901778481
5	16.240210640021605	32.57879711430886	34.6258824891015	16.555109756568033
6	31.732475598935228	35.1240307086918	16.06274835075807	17.080745341614907
7	28.92249527410208	28.048686393271865	20.435168396281007	22.59364993634505
8	27.02297365070792	29.63861347941823	21.089078353458586	22.24933451641526
9	28.88488098452992	11.815709270475676	18.05534122911925	41.244068515875156
10	19.939431349099184	22.54301531576714	28.81013463986729	28.707418695266384
11	39.25726245129432	19.209328343813898	18.116102773812738	23.417306431079048
12	22.520350295127503	24.608425600864166	25.915281046255934	26.955943057752403
13	36.00893098260098	17.507040623432736	19.896994714710083	26.5870336792562
14	23.78669804405694	20.922707457274026	23.660834844334712	31.629759654334322
15	30.743412676980054	23.558118899733806	20.280371127657112	25.41809729562903
16	24.594440800895025	24.265074649897766	22.857432197831873	28.283052351375332
17	26.825257513213224	23.90532772655376	21.11174337409822	28.15767138613479
18	26.92459781644227	20.630955595848928	22.844411866826125	29.600034720882682
19	28.448458778596503	21.934435399868832	23.235021796998574	26.38208402453609
20	31.63072412329771	19.376663708961843	22.60811697079588	26.384495196944563
21	27.584776821881874	21.743952779599553	21.497530959453726	29.17373943906485
22	29.42498360402762	22.568091508815247	20.8648393194707	27.14208556768643
23	28.09883877936808	21.933953165387138	22.13263377184522	27.83457428339956
24	27.81914277998534	21.231337525558427	22.938929825238226	28.010589869218013
25	27.721731414683077	21.71935882103314	23.104336252459394	27.454573511824385
26	28.040488407083057	22.913853632190115	21.846668724200455	27.198989236526366
27	26.697465375564217	21.429053663053125	24.973959337988504	26.899521623394158
28	25.937946066895567	22.522761467535975	21.894892172369893	29.644400293198565
29	27.591045870143898	23.335326569191004	22.33710119208364	26.73652636858146
30	26.954014119825626	21.099687512055862	23.98248524362486	27.963813124493655
31	28.199143551560514	22.606670267350797	22.75905636356622	26.435129817522473
32	25.705991281200568	22.13697388218047	22.546873191620694	29.61016164499826
33	25.37324948883145	21.348038270128466	25.486092357547935	27.792619883492147
34	26.24320049380811	21.36829211835963	24.50136954592801	27.88713784190425
35	25.14081246865476	24.078932139963737	24.802766096987	25.977489294394505
36	26.106245901006908	22.70842174298831	25.030380772346746	26.154951583658036
37	26.52771883800779	23.03971683191235	24.40444041510744	26.02812391497242
38	26.01510358396667	21.857760117279426	25.071370703290768	27.05576559546314
39	25.335635199259286	20.709077581883413	26.0551290459473	27.900158172909993
40	24.88088808302149	22.216542571660046	27.012364492110645	25.89020485320782
41	23.20946336946877	20.598163651093707	27.89485359361136	28.297519385826163
42	21.13344392577447	21.2163882566259	29.76351221017708	27.886655607422554
43	20.636260175147562	22.069943289224952	28.872825122487562	28.420971413139924
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	2.0
2	0.0
3	8.0
4	16.0
5	16.0
6	19.5
7	23.0
8	21.0
9	19.0
10	33.5
11	48.0
12	48.0
13	104.0
14	160.0
15	310.5
16	461.0
17	434.0
18	407.0
19	407.0
20	446.5
21	486.0
22	355.0
23	224.0
24	213.0
25	202.0
26	202.0
27	206.0
28	210.0
29	289.0
30	368.0
31	563.0
32	758.0
33	758.0
34	1218.0
35	1678.0
36	1926.0
37	2174.0
38	3085.5
39	3997.0
40	3997.0
41	4765.5
42	5534.0
43	7073.5
44	8613.0
45	12945.5
46	17278.0
47	17278.0
48	19465.5
49	21653.0
50	24573.5
51	27494.0
52	27388.0
53	27282.0
54	27282.0
55	24165.0
56	21048.0
57	19674.5
58	18301.0
59	16833.0
60	15365.0
61	15365.0
62	13465.5
63	11566.0
64	9139.0
65	6712.0
66	5609.5
67	4507.0
68	4507.0
69	3743.0
70	2979.0
71	2570.5
72	2162.0
73	1746.5
74	1331.0
75	1331.0
76	1200.5
77	1070.0
78	1015.0
79	960.0
80	909.5
81	859.0
82	859.0
83	795.0
84	731.0
85	516.5
86	302.0
87	277.5
88	253.0
89	253.0
90	168.5
91	84.0
92	63.0
93	42.0
94	24.5
95	7.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	207368.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.12721345627098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.20694379348883	32.18481154276455
2	8.171996490848565	6.558388951043555
3	3.176262753722465	3.8236372053547316
4	1.7726021800002403	2.8451834419968365
5	1.1236495174916779	2.254446201921222
6	0.8652702166780833	2.083252960919718
7	0.5612238766509235	1.5764245206589251
8	0.4566703921356551	1.4659928243509124
9	0.3941786542644602	1.423556189961807
>10	2.7832858636478353	22.081516916785617
>50	0.3148622177356359	8.840322518421358
>100	0.16103640143730996	11.746267505111685
>500	0.012017641898306713	3.1161992207090776
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	857	0.41327495081208293	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	816	0.3935033370626133	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	724	0.3491377647467305	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	671	0.32357933721692833	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	590	0.28451834419968364	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	583	0.281142702827823	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	574	0.27680259249257355	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	559	0.2695690752671579	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	545	0.26281779252343657	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	543	0.26185332356004787	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	478	0.23050808224991318	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	402	0.19385826164114037	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	398	0.1919293237143629	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	370	0.17842675822692028	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	357	0.17215770996489332	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	345	0.16637089618456077	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	325	0.1567262065506732	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	318	0.15335056517881254	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	313	0.15093939277034063	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	300	0.1446703445083137	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	298	0.14370587554492495	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	292	0.1408124686547587	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	292	0.1408124686547587	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	288	0.13888353072798118	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	288	0.13888353072798118	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	287	0.13840129624628678	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	285	0.13743682728289802	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	279	0.13454342039273176	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	279	0.13454342039273176	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	274	0.13213224798425985	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	272	0.1311677790208711	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	270	0.13020331005748237	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	265	0.12779213764901046	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	263	0.1268276686856217	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	258	0.1244164962771498	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	257	0.12393426179545543	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	250	0.12055862042359476	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	247	0.11911191697851163	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	245	0.11814744801512288	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	244	0.1176652135334285	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	244	0.1176652135334285	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	243	0.11718297905173411	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	240	0.11573627560665098	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG	235	0.11332510319817908	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	234	0.1128428687164847	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	231	0.11139616527140157	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	228	0.10994946182631843	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	224	0.10802052389954091	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	223	0.10753828941784654	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	223	0.10753828941784654	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	221	0.10657382045445778	No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT	220	0.10609158597276339	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	219	0.10560935149106902	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	217	0.10464488252768026	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	215	0.1036804135642915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.8223448169437905E-4	0.0	0.0	0.0	0.0
9	4.8223448169437905E-4	0.0	0.0	0.0	0.0
10	9.644689633887581E-4	0.0	0.0	0.0	0.0
11	9.644689633887581E-4	0.0	0.0	0.0	0.0
12	9.644689633887581E-4	0.0	0.0	0.0	0.0
13	9.644689633887581E-4	0.0	0.0	0.0	0.0
14	9.644689633887581E-4	0.0	0.0	0.0	0.0
15	9.644689633887581E-4	0.0	0.0	0.0	0.0
16	9.644689633887581E-4	0.0	0.0	4.8223448169437905E-4	0.0
17	0.0014467034450831372	0.0	0.0	0.0019289379267775162	0.0
18	0.0014467034450831372	0.0	0.0	0.0038578758535550324	0.0
19	0.0014467034450831372	0.0	0.0	0.0048223448169437905	4.8223448169437905E-4
20	0.0014467034450831372	0.0	0.0	0.006269048262026928	4.8223448169437905E-4
21	0.0014467034450831372	0.0	0.0	0.009644689633887581	4.8223448169437905E-4
22	0.0014467034450831372	0.0	0.0	0.013984799969136994	4.8223448169437905E-4
23	0.0014467034450831372	0.0	0.0	0.016395972377608887	4.8223448169437905E-4
24	0.0014467034450831372	0.0	0.0	0.019289379267775162	4.8223448169437905E-4
25	0.0014467034450831372	0.0	0.0	0.022665020639635818	4.8223448169437905E-4
26	0.0014467034450831372	0.0	0.0	0.027005130974885227	4.8223448169437905E-4
27	0.0014467034450831372	0.0	0.0	0.04967015161452105	4.8223448169437905E-4
28	0.0014467034450831372	0.0	0.0	0.13888353072798118	4.8223448169437905E-4
29	0.0014467034450831372	0.0	0.0	0.3134524131013464	4.8223448169437905E-4
30	0.0014467034450831372	0.0	0.0	0.5439604953512596	4.8223448169437905E-4
31	0.0014467034450831372	0.0	0.0	1.0917788665560741	4.8223448169437905E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGA	25	1.2301387E-4	37.0	17
CAGTATT	25	1.2301387E-4	37.0	14
ATTGACT	30	8.279147E-6	37.0	31
TATTGAC	35	2.3789471E-5	31.714287	30
AGTATTA	30	3.5894016E-4	30.833332	15
TAAACCA	25	0.0054860874	29.6	5
CGTAAAA	25	0.0054860874	29.6	32
GCCGTCC	25	0.0054860874	29.6	25
CTCGTAA	35	8.844798E-4	26.428572	30
ATTAGAG	35	8.844798E-4	26.428572	18
CTACGGG	35	8.844798E-4	26.428572	11
AACGACC	50	9.044254E-6	25.900002	37
CTCTATG	130	0.0	25.615385	1
GGTATCA	465	0.0	25.064518	1
GGGCAGT	115	0.0	24.130436	7
AAACACG	70	1.9071558E-7	23.785715	15
TCTATGG	140	0.0	23.785715	2
GCAGTCG	110	3.6379788E-12	23.545454	9
CAGTCGG	110	3.6379788E-12	23.545454	10
CCTACGG	40	0.0019258828	23.125	10
>>END_MODULE