FastQCFastQC Report
Fri 10 Feb 2017
ERR1631664.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631664.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29637
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC560.18895299794176199No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA490.16533387319904175No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA470.1585855518439788No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC430.14508890913385294No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA410.13834058777879002No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA410.13834058777879002No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT410.13834058777879002No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT410.13834058777879002No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA410.13834058777879002No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG400.13496642710125856No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT390.1315922664237271No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA390.1315922664237271No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT340.11472146303606978No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC320.10797314168100684No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA300.1012248203259439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAC303.5161883E-430.8333325
TGACCAT303.5161883E-430.8333328
AGTGACC303.5161883E-430.8333326
TTAGTGA303.5161883E-430.8333324
TTATGAG250.00541133729.63
GGTATCA453.8377148E-628.7777771
TCTTATA652.746674E-928.46153637
AGGCGGC358.6660794E-426.4285728
TGTGGGG358.6660794E-426.42857218
GTTGGGT358.6660794E-426.42857219
GTGTGGG358.6660794E-426.42857217
GAGGCGG400.001887334523.1257
TTGGGTT400.001887334523.12520
GTGACCA400.001887334523.1257
TGCAGGA400.001887334523.1252
GGTGTGG400.001887334523.12516
GGCGGCG400.001887334523.1259
GCCTGTC603.5710007E-521.58333237
CTTTAGT450.003739752620.5555552
GGCTGCC450.003739752620.55555523
Produced by FastQC (version 0.11.5)